Cysteine synthase (O-acetylserine (thiol) lyase) substrate specificities classify the mitochondrial isoform as a cyanoalanine synthase

Journal of Experimental Botany

Volumn 51, Issue 347, 2000, Pages 985-993

  Warrilow, Andrew G S ^a   Hawkesford, Malcolm J ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

Compartmentation; Cyanoalanine synthase; Cysteine synthase; Enzyme kinetics; O acetylserine (thiol) lyase

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; CATALYSIS; CYANIDE; CYANOALANINE SYNTHASE; CYSTEINE SYNTHASE; ENZYME ISOFORM; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; MICHAELIS MENTEN KINETICS; MITOCHONDRION; SEQUENCE HOMOLOGY; SPINACH; SULFIDE;

SPINACIA OLERACEA;

EID: 0034121969     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jexbot/51.347.985     Document Type: Article

References (35)

1. Bertagnolli BL, Wedding RT. 1977. Purification and initial characterization of different forms of o-acetylserine sulfhydrylase from seedings of two species of Phaseolus. Plant Physiology 60, 115-121.
2. Cook PF, Wedding RT. 1976. A reaction mechanism from steady state kinetic studies for o-acetylserine sulfhydrylase from Salmonella typhimurium LT-2. Journal of Biological Chemistry 251, 2023-2029.
3. Gaitonde MK. 1967. A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochemical Journal 104, 627-633.
4. Hasegawa R, Maruyama A, Nakaya M, Tsuda S, Esashi Y. 1995. The presence of two types of β-cyanoalanine synthase in germinating seeds and their responses to ethylene. Physiologica Plantarum 93, 713-718.
5. Hasegawa R, Tomoko T, Yuichiro T, Yohji E. 1994. Presence of β-cyanoalanine synthase in unimbibed dry seeds and its activation by ethylene during pre-germination. Physiologica Plantarum 91, 141-146.
6. Hawkesford MJ, Belcher AR. 1991. Differential protein synthesis in response to sulphate and phosphate deprivation: identification of possible components of plasma-membrane transport systems in cultured tomato roots. Planta 185, 323-329.
7. Hell R, Schuster G, Gruissem W. 1993. An O-acetylserine (thiol) lyase cDNA from spinach. Plant Physiology 102, 1057-1058.
8. Hendrickson HR, Conn EE. 1969. Cyanide metabolism in higher plants. IV. Purification and properties of the β-cyanoalanine synthase of blue lupine. Journal of Biological Chemistry 244, 2632-2640.
9. Ikegami F, Itagaki S, Murakoshi I. 1993. Purification and characterization of two forms of cysteine synthase from Allium tuberosum. Phytochemistry 32, 31-34.
10. Ikegami F, Kaneko M, Kamiyama H, Murakoshi I. 1988b. Purification and characterization of cysteine synthases from Citrullus vulgaris. Phytochemistry 27, 697-701.
11. Ikegami F, Kaneko M, Kobori M, Murakoshi I. 1988a. Purification and characterization of cysteine synthase from Brassica juncea. Phytochemistry 27, 3379-3383.
12. Ikegami F, Takayama K, Murakoshi I. 1988d. Purification and properties of β-cyano-L-alanine synthase from Lathyrus latifolius. Phytochemistry 27, 3385-3389.
13. Ikegami F, Takayama K, Tajima C, Murakoshi I. 1988c. Purification and properties of β-cyano-L-alanine synthase from Spinacia oleracea. Phytochemistry 27, 2011-2016.
14. Kuske CR, Ticknor LO, Guzmán E, Gurley LR, Valdez JG, Thompson ME, Jackson PJ. 1994. Purification and characterization of O-acetylserine sulfhydrylase isoenzymes from Datura innoxia. Journal of Biological Chemistry 269, 6223-6232.
15. Kuske CR, Hill KK, Guzmán E, Jackson PJ. 1996. Subcellular location of O-acetylserine sulfhydrylase isoenzymes in cell cultures and plant tissues of Datura innoxia Mill. Plant Physiology 112, 659-667.
16. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
17. Lunn JE, Droux M, Martin J, Douce R. 1990. Localization of ATP sulfurylase and O-acetylserine (thiol) lyase in spinach leaves. Plant Physiology 94, 1345-1352.
18. Murakoshi I, Ikegami F, Kaneko M. 1985. Purification and properties of cysteine synthase from Spinacia oleracea. Phytochemistry 24, 1907-1911.
19. Nakamura T, Yamaguchi Y, Sano H. 1999. Four rice genes encoding cysteine synthase: isolation and differential responses to sulfur, nitrogen and light. Gene 229, 155-161.
20. Ng BH, Anderson JW. 1978. Chloroplast cysteine synthases of Trifolium repens and Pisum sativum. Phytochemistry 17, 879-885.
21. Ngo TT, Shargool PD. 1973. The use of a sulfide ion selective electrode to study o-acetylserine sulfhydrylase from germinating rapeseed. Analytical Biochemistry 54, 247-261.
22. Rolland N, Droux M, Douce R. 1992. Subcellular distribution of O-acetylserine (thiol)lyase in cauliflower (Brassica oleracea L.) inflorescence. Plant Physiology 98, 927-935.
23. Rolland N, Droux M, Lebrun M, Douce R. 1993. O-acetylserine(-thiol)lyase in from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization and overexpression in Escherichia coli of the chloroplast isoform. Archives of Biochemistry and Biophysics 300, 213-222.
24. Rolland N, Ruffet ML, Job D, Douce R, Droux M. 1996. Spinach chloroplast O-acetylserine(thiol)lyase exhibits two catalytically non-equivalent pyridoxal-5′-phosphate containing active sites. European Journal of Biochemistry 236, 272-282.
25. Rosichan JL, Blake N, Stallard R, Owais WM, Kleinhofs A, Nilan RA. 1983. O-acetylserine (thiol)-lyase from barley converts sodium azide to a mutagenic metabolite. Biochimica et Biophysica Acta 748, 367-373.
26. Saito K, Miura N, Yamazaki M, Hirano H, Murakoshi I. 1992. Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase. Proceedings of the National Academy of Sciences, USA 89, 8078-8082.
27. Saito K, Tatsuguchi K, Murakoshi I, Hirano H. 1993. cDNA cloning and expression of cysteine synthase B localized in chloroplasts of Spinacia oleracea. FEBS Letters 324, 247-252.
28. Saito K, Kurosawa M, Tatsuguchi K, Takagi Y, Murakoshi I. 1994a. Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine(thiol)lyase]. Plant Physiology 106, 887-895.
29. Saito K, Tatsuguchi K, Takagi Y, Murakoshi I. 1994b. Isolation and characterization of cDNA that encodes a putative mitochondrion-localised of cysteine synthase (O-acetylserine-(thiol)lyase) from Spinacia oleracea. Journal of Biological Chemistry 269, 28187-28192.
30. Segel IH. 1993. Enzyme kinetics: behavior and analysis of rapid equilibrium and steady-state enzyme systems, 2nd edn. New York: John Wiley and Sons.
31. Smith FW, Hawkesford MJ, Ealing PM, Clarkson DT, Vanden Berg PJ, Belcher AR, Warrilow AGS. 1997. Regulation of expression of a cDNA from barley roots encoding a high affinity sulphate transporter. The Plant Journal 12, 875-884.
32. Tai CH, Nalabolu SR, Jacobson TM, Minter DE, Cook PF. 1993. Kinetic mechanisms of the A and B isozymes of o-acetylserine sulfhydrylase from Salmonella typhimurium LT-2 using the natural and alternative reactions. Biochemistry 32, 6433-6442.
33. Tai CH, Nalabolu SR, Simmons JW, Jacobson TM, Cook PF. 1995. Acid-base chemical mechanism of O-acetylserine sulfhydrylase-A and -B from pH studies. Biochemistry 34, 12311-12322.
34. Warrilow AGS, Hawkesford MJ. 1998. Separation, subcellular location and influence of sulphur nutrition on isoforms of cysteine synthase in spinach. Journal of Experimental Botany 49, 1625-1636.
35. Wurtele ES, Nikolau BJ, Conn EE. 1985. Subcellular and developmental distribution of β-cyanoalanine synthase in barley leaves. Plant Physiology 78, 285-290.