Spinach chloroplast O-acetylserine (thiol)-lyase exhibits two catalytically non-equivalent pyridoxal-5'-phosphate-containing active sites

European Journal of Biochemistry

Volumn 236, Issue 1, 1996, Pages 272-282

  Rolland, N ^a   Ruffet, M L ^a   Job, D ^a   Douce, R ^a   Droux, M ^a  

^a CNRS   (France)

Author keywords

Chloroplasts; Cysteine; O acetylserine (thiol) lysate; Pyridoxal 5' phosphate; Sulfur

Indexed keywords

CYSTEINE SYNTHASE; FLUORESCENT DYE; LYASE; MET17 PROTEIN, S CEREVISIAE; MULTIENZYME COMPLEX; O ACETYLHOMOSERINE (THIOL) LYASE; O-ACETYLHOMOSERINE (THIOL)-LYASE; PYRIDOXAL 5 PHOSPHATE; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; SYNTHETIC DNA; TRYPTOPHAN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHLOROPLAST; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE; GENE CONSTRUCT; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; SPECTROPHOTOMETRY; SPINACH; TECHNIQUE; AMINO ACID SEQUENCE; BINDING SITE; CATALYSIS; CHEMICAL MODEL; CHEMISTRY; ENZYMOLOGY; FLUOROMETRY; GENE; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; TITRIMETRY;

SPINACIA OLERACEA;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CARBON-OXYGEN LYASES; CATALYSIS; CHLOROPLASTS; CYSTEINE SYNTHASE; ESCHERICHIA COLI; FLUORESCENT DYES; FLUOROMETRY; GENES, PLANT; GENES, SYNTHETIC; KINETICS; LYASES; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PROTEIN CONFORMATION; PYRIDOXAL PHOSPHATE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SPINACIA OLERACEA; TITRIMETRY; TRYPTOPHAN;

EID: 0029664542     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00272.x     Document Type: Article

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