Optical detection methods for combinatorial libraries

Current Opinion in Chemical Biology

Volumn 4, Issue 3, 2000, Pages 351-355

  Gauglitz, Günter ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

CHEMICAL ANALYSIS; FLUORESCENCE ANALYSIS; MOLECULAR INTERACTION; OPTICAL RESOLUTION; ORGANIC CHEMISTRY; REVIEW; TECHNOLOGY;

EID: 0034115344     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(00)00099-5     Document Type: Review

References (46)

1. Dulery B.D., Verne-Mismer J., Wolf E., Kugel C., Van Hijfte L. Analyses of compound libraries obtained by high-throughput parallel synthesis: strategy of quality control by high-performance liquid chromatography, mass spectrometry and nuclear magnetic resonance techniques. J Chromatogr B. 725:1999;39-47. This article gives strategies to characterise leads, and for quality control. Information on automated, fast reversed-phase C18 HPLC with diode-array detection, followed by atmospheric pressure chemical ionisation MS and NMR is given. Validated libraries are registered in ISIS databases using automated procedures.
2. Süssmuth R.D., Jung G. Impact of mass spectrometry on combinatorial chemistry. J Chromatogr B. 725:1999;49-65. This article reviews new MS methodologies for HTS and the coupling of these methods to separation techniques (e.g. HPLC and capillary electrophoresis). Recent developments in Fourier transform ion cyclotron resonance are also discussed.
3. Keough T., Youngquist R.S., Lacey M.P. A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionisation mass spectrometry. Proc Natl Acad Sci USA. 96:1999;7131-7136.
4. Hsu B.H., Orton E., Tang S.Y., Carlton R.A. Application of evaporative light scattering detection to the characterisation of combinatorial and parallel synthesis libraries for pharamceutical drug discovery. J Chromatogr B. 725:1999;103-112.
5. MacNamara E., Hou T., Fisher G., Williams S., Raftery D. Multiplex sample NMR: an approach to high-throughput NMR using a parallel coil probe. Anal Chim Acta. 397:1999;9-16.
6. Fejzo J., Lepre C.A., Peng J.W., Bemis G.W., Murcko M.A., Moore J.M. The SHAPES strategy: an NMR-based approach for lead generation in drug discovery. Chem Biol. 6:1999;755-769.
7. Ehrfeld W., Brüning H., Kiesewalter Status and perspectives for growth in microtechnology. MST News. 1999;4-7. This study describes the status of microtechnology, especially at the Institute of Microtechnology Mainz (Mainz, Germany). Potential applications of newly developed micro-structured devices are reviewed. These include LIGA technology, microfluidics and microstructured reactors.
8. Microtechnologies and Miniaturisation: Tools, Techniques and Novel Applications for the Pharmaceutical Industry. 1999;IBC UK Conferences, Ltd, London.
9. Konrad R., Hartmann H.J., Jacob P., Pommersheim R., Sommer I., Neumann M., Wolfrum J. Durchlauf in Rekordzeit: Chip für die Hochdurchsatzanalyse von Biomolekülen. BioTec. 3:1999;44-46. [Title translation: Throughput at minimum time: chips for high-throughput analysis of biomolecules.].
10. Cohen C.B., Chin-Dixon E., Jeong S., Nikiforov T.T. A microchip-based enzyme assay for protein kinase A. Anal Biochem. 273:1999;89-97.
11. Behr S., Matzig M., Levin A., Eickhoff H., Heller C. A fully automated multicapillary electrophoresis device for DNA analysis. Electrophoresis. 20:1999;1492-1507.
12. The Agilent Technologies lab-on-a-chip web page on URL: http://www.hp.com/go/labonachip.
13. The Bio/Chemical Microsystems Research Group homepage on URL: http://www.mic.dtu.dk/mic/research/bcms/biochem.htm.
14. Venton D.L., Woodbury C.P. Screening combinatorial libraries. Chemometrics Int Lab Systems. 48:1999;131-150. This article reviews the various pooling strategies that are presently being employed. The use of microorganisms in library screening, as well as DNA and RNA screening, are reviewed. In addition, both labelled and non-labelled detection strategies to relate structure and activity are discussed.
15. Xue G., Pang H.M., Yeung E.S. Multiplexed capillary zone electrophoresis and micellar electrokinetic chromatography with internal standardization. Anal Chem. 71:1999;2642-2649.
16. Nielson, J.: The UHTS system from Carl Zeiss: first user experiences with the UHTS prototype. Proceedings of the Society of Biomolecular Screening Fifth Annual Conference and Exhibition: 1999 Sept 13-16; Edinburgh, Scotland:74.
17. Ramm P. Imaging systems in assay screening. Drug Discov Today. 4:1999;401-410.
18. Amersham Pharmacia Biotech: an introduction to drug discovery and development services on URL: http://apbiotech.com/product/product_index.html.
19. Abriola L., Chin M., Fürst P., Schweitzer R., Sills M.A. Digital imaging as a detection method for a fluorescent protease assay in 96-well and miniaturized assay plate formats. J Biomol Screen. 4:1999;121-127. Digital imaging is discussed as a detection method that can measure fluorescent or luminescent signals in miniaturized formats. In this study, an imaging system capable of detecting the signal from a fluorescent protease assay in multiple plate formats was used to evaluate this detection method in an HTS environment. It was evaluated in plates with 96, 384, 664 and 1536 wells using imaging detection.
20. Maffia A.M., Kariv I., Oldenburg K.R. Miniaturization of a mammalian cell-based assay: luciferase reporter gene readout in a 3 microliter 1536-well plate. J Biomol Screen. 4:1999;137-142.
21. Roda A., Pasini P., Mirasoli M., Guardigli M., Russo C., Musiani M., Mazzeo C. Chemiluminescent imaging in immunoassays and DNA detection. J Clin Ligand Assay. 21:1998;365-376.
22. Plowman T.E., Durstchi J.D., Wang H.K., Christensen D.A., Herron J.N., Reichert W.M. Multiple-analyte fluoroimmunoassay using an integrated optical waveguide sensor. Anal Chem. 71:1999;4344-4352.
23. Mallat E., Barzen C., Klotz A., Brecht A., Gauglitz G., Barcelo D. River analyzer for chlorotriazines with a direct optical immunosensor. Environ Sci Technol. 33:1999;965-971. In an application of multi-analyte and multi-sample detection in environmental analysis, a prototype based on total internal reflection fluorescence is given. The features of the prototype are at present restricted to six samples; however, the potential of HTS is obvious using advanced newly structured integrated optical devices.
24. Sapolsky R.J., Hsie L., Berno A., Ghanndour G., Mittmann M., Fan J.B. High-throughput polymorphism screening and genotyping with high-density oligonucleotide arrays. Genet Anal. 14:1999;187-192.
25. Pope A.J., Haupts U.M., Moore K.J. Homogeneous fluorescence readouts for miniaturised high-throughput screening: theory and practise. Drug Discov Today. 4:1999;350-362.
26. Ambrose W.P., Semin D.J., Robbins D.L., Van Orden A., Kashem M.A., Hamilton S.A., Nelson R.M., Jett J.H., Keller R.A. Detection system for reaction-rate analysis in a low-volume proteinase-inhibition assay. Anal Biochem. 263:1998;150-157.
27. ™) to monitor poly-ubiquitination of wild-type p53. Comb Chem High Throughput Screen. 2:1999;279-287.
28. Park Y.W., Cummings R.T., Wu L., Zheng S., Cameron P.M., Woods A., Zaller D.M., Marcy A.I., Hermes J.D. Homogeneous proximity tyrosine kinase assays: scintillation proximity assay versus homogeneous time-resolved fluorescence. Anal Biochem. 269:1999;94-104.
29. Nasir M.S., Jolley M.E. Fluorescence polarization: an analytical tool for immunoassay and drug discovery. Comb Chem High Throughput Screen. 2:1999;177-190.
30. Gauglitz G. Optical sensor arrays based on microtiterplate dimensions. Mikrochim Acta. 131:1999;9-17. Review of the principles and feasibilities of some optical detection methods, such as resonance energy transfer, total internal reflection fluorescence and reflectometric interference spectroscopy. Some applications are given and the potential for HTS is discussed.
31. Schobel U., Egelhaaf H.J., Brecht A., Oelkrug D., Gauglitz G. New donor-acceptor pair for fluorescent immunoassays by energy transfer. Bioconj Chem. 10:2000;1107-1114.
32. Coille I, Schobel U, Gauglitz G: Fluorescence detection in high-throughput-analysis. Proceedings of the Third Euroconference on Environmental Analalytical Chemistry: 1999 Oct 9-15; Chalkidiki, Greece.
33. Gauglitz G, Coille I, Schobel U: Hoher Probendurchsatz in der Umweltanalytik durch Immunoassay im Nanoliterbereich. Proc. 4. Dresdner Sensor-Symposium, Dez. 1999, in press.
34. Biacore homepage on URL: http://www.biacore.com.
35. Berger C.E.H., Beumer T.A.M., Kooyman R.P.H., Greve J. Surface plasmon resonance multisensing. Anal Chem. 70:1998;703-706.
36. Fischer M., Tran C.D. Investigation of solid phase peptide synthesis by the near-infrared multispectral imaging technique: a detection method for combinatorial chemistry. Anal Chem. 71:1999;2255-2261.
37. Kell DB: Reagentless high throughput assay. Proceedings of the Society of Biomolecular Screening Fifth Annual Conference and Exhibition: 1999 Sept 13-16; Edinburgh, Scotland:11.
38. Shaw A.D., Katerbhai N., Jones A., Woodward A.M., Goodacre R., Rowland J., Kell D.B. Non-invasive, on-line monitoring of the biotransformation by yeast of glucose to ethanol using non-dispersive Raman spectroscopy and chemometrics. Appl Spectr. 53:1999;1419-1428.
39. The Aberystwyth quantative biology and analytical biotechnology group homepage on URL: http://gepasi.dbs.aber.ac.uk.
40. Gremlich H.U. The use of optical spectroscopy in combinatorial chemistry. Biotech Bioengineer. 61:1998;179-187.
41. Rothmund M., Schütz A., Brecht A., Gauglitz G., Berthel G., Gräfe D. Label free binding assay with spectroscopic detection for pharmaceutical screening. Fresenius J Anal Chem. 359:1997;15-22.
42. Brecht A., Gauglitz G., Nahm W. Interferometric measurements used in chemical and biochemical senors. Analusis. 20:1992;135-140.
43. Tünnemann R, Schmitt HM, Walk T, Brecht A, Gauglitz G, Jung G, Austel V: Online monitoring of solid phase peptide synthesis using white light interference. European Peptide Symposium, Prague 1999.
44. Sauer M., Brecht A., Charisse K., Stemmler I., Gauglitz G., Bayer E. Interaction of chemically modified antisense oligonucleotides with sense DNA: a label free interaction study. Anal Chem. 71:1999;2850-2857. Reflectometric interference spectroscopy is introduced as a successful tool for direct optical monitoring of hybridisation without use of labelled compounds. The determination of binding by time-resolved measurement enables discrimination between perfect matches and mismatches.
45. Pope A.J., Hertzberg R. HTS: a retrospective look at screening in the first decade of the new millenium. J Biomol Screen. 4:2010;231-233.
46. Burbaum J.J. The evolution of miniaturized well plates. J Biomol Screen. 5:2000;5-8.