Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida

Infection and Immunity

Volumn 66, Issue 5, 1998, Pages 1990-1998

  Vipond, Richard ^a,c   Bricknell, Ian R ^b   Durant, Emma ^a   Bowden, Timothy J ^b   Ellis, Anthony E ^b   Smith, Mary ^a   MacIntyre, Sheila ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b MARINE LABORATORY   (United Kingdom)

^c PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCEROPHOSPHOLIPID;

AEROMONAS SALMONICIDA; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; FISH; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

AEROMONAS SALMONICIDA;

EID: 0031922263     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.5.1990-1998.1998     Document Type: Article

References (37)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Current protocols in molecular biology. John Wiley & Sons, Chichester, United Kingdom.
2. Braun, V., and G. Schmitz. 1980. Excretion of a protease by Serratia marcescens. Arch. Microbiol. 124:55-61.
3. Bricknell, I. Unpublished data.
4. Bricknell, I. R. 1995. A reliable method for the induction of experimental furunculosis. J. Fish Dis. 18:127-133.
5. Bricknell, I. R., T. J. Bowden, J. Lomax, and A. E. Ellis. 1997. Antibody response and protection of Atlantic salmon (Salmo salar) immunised with an extracellular polysaccharide of Aeromonas salmonicida. Fish Shellfish Immunol. 7:1-16.
6. Brumlick, M. J., and J. T. Buckley. 1996. Identification of the catalytic triad of the lipase/acyltransferase from Aeromonas hydrophila. J. Bacteriol. 178: 2060-2064.
7. Buckley, J. T. 1990. Purification of cloned proaerolysin released by a low protease mutant of Aeromonas salmonicida. Biochem. Cell Biol. 68:221-224.
8. Buckley, J. T., L. N. Halasa, and S. MacIntyre. 1982. Purification and partial characterisation of a bacterial phospholipid: cholesterol acyltransferase. J. Biol. Chem. 255:3320-3325.
9. Chavira, R., T. J. Burnett, and J. H. Hageman. 1984. Assaying proteinases with Azocoll. Anal. Biochem. 136:446-450.
10. Coleman, G., and P. W. Whitby. 1993. A comparison of the amino acid sequence of the serine protease of the fish pathogen Aeromonas salmonicida subsp. salmonicida with those of other subtilisin-type enzymes relative to their substrate-binding sites. J. Gen. Microbiol. 139:245-249.
11. Cornelis, G. R., and H. Wolf-Watz. 1997. The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cells. Mol. Microbiol. 23:861-867.
12. Costello, G., R. Vipond, and S. MacIntyre. 1996. Aeromonas salmonicida possesses two genes encoding homologues of the major outer membrane protein, OmpA. J. Bacteriol. 178:1623-1630.
13. Drinan, E. M., and P. R. Smith. 1985. Histopathology of a mutant Aeromonas salmonicida infection in Atlantic salmon (Salmo salar), p. 79-83. In A. E. Ellis (ed.), Fish and shellfish pathology. Academic Press Ltd., London, United Kingdom.
14. Eggset, G., R. Bjornsdottir, R. McQueen Leifson, J. A. Arnesen, D. H. Coucheron, and T. O. Jorgensen. 1994. Extracellular glycerophospholipid: cholesterol acyltransferase from Aeromonas salmonicida: activation by serine protease. J. Fish Dis. 17:17-29.
15. Ellis, A. E. 1997. The extracellular toxins of Aeromonas salmonicida ssp. salmonicida, p. 248-268. In E.-M. Bernoth, A. E. Ellis, P. J. Midtlyng, G. Olivier, and P. Smith (ed.), Furunculosis. Multidisciplinary fish disease research. Academic Press Ltd., London, United Kingdom.
16. Ellis, A. E., A. do Vale, T. J. Bowden, K. Thompson, and T. S. Hastings. 1997. In vivo production of A-protein, lipopolysaccharide, iron-regulated outer membrane proteins and 70 kDa serine protease by Aeromonas salmonicida subsp. salmonicida. FEMS Microbiol. Lett. 149:157-163.
17. Fiore, A. E., J. M. Michalski, R. G. Russell, C. L. Sears, and J. B. Kaper. 1997. Cloning, characterisation and chromosomal mapping of a phospholipase (lecithinase) produced by Vibrio cholerae. Infect. Immun. 65:3112-3117.
18. Furste, J. P., W. Pansegrau, R. Frank, H. Blocker, P. Scolz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the plasmid RF4 primase region in a multihost-range expression vector. Gene 48:119-131.
19. Fyfe, L., G. Coleman, and A. L. S. Munro. 1988. The combined effect of isolated Aeromonas salmonicida protease and haemolysin on Atlantic salmon, Salmo salar L., compared with that of a total extracellular products preparation. J. Fish Dis. 11:101-104.
20. Hilton, S., W. D. McCubbin, C. M. Kay, and J. T. Buckley. 1990. Purification and spectral study of a microbial fatty acyltransferase: activation by limited proteolysis. Biochemistry 29:9072-9078.
21. Hirono, I., and T. Aoki. 1993. Cloning and characterisation of three hemolysin genes from Aeromonas salmonicida. Microb. Pathog. 15:269-282.
22. Howard, S. P., S. MacIntyre, and J. T. Buckley. 1996. Toxins, p. 267-281. In B. Austin, M. Altwegg, P. J. Gosling, and S. Joseph (ed.), The genus Aeromonas. John Wiley & Sons, Chiehester, United Kingdom.
23. Karlyshev, A. V., and S. MacIntyre. 1995. Cloning and study of the genetic organisation of the exe gene cluster of Aeromonas salmonicida. Gene 158: 77-82.
24. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
25. Lee, K.-K., and A. E. Ellis. 1990. Glycerophospholipid:cholesterol acyltransferase complexed with lipopolysaccharide (LPS) is a major lethal exotoxin and cytolysin of Aeromonas salmonicida LPS stabilizes and enhances the toxicity of the enzyme. J. Bacteriol. 172:5382-5393.
26. MacIntyre, S., T. J. Trust, and J. T. Buckley. 1979. Distribution of glycerophospholipid:cholesterol acyltransferase in selected bacterial species. J. Bacteriol. 139:132-136.
27. McCarthy, D. H. 1983. An experimental model for fish furunculosis caused by Aeromonas salmonicida. J. Fish Dis. 6:231-237.
28. Nerland, A. H. 1996. The nucleotide sequence of the gene encoding GCAT from Aeromonas salmonicida ssp. salmonicida. J. Fish Dis. 19:145-150.
29. Nieto, T. P., Y. Santos, L. A. Rodriguez, and A. E. Ellis. 1991. An extracellular acetylcholinesterase produced by Aeromonas hydrophila is a major lethal toxin for fish. Microb. Pathog. 11:101-110.
30. Sakai, D. K. 1985. Loss of virulence in a protease-deficient mutant of Aeromonas salmonicida. Infect. Immun. 48:146-152.
31. 2,-macroglobulin. J. Fish Dis. 15:215-227.
32. Selbitschka, W., N. Stefan, and A. Puhler. 1993. Construction of gene replacement vectors for Gram -ve bacteria using a genetically modified sacRB gene as a positive selection marker. Appl. Microbiol. Biotechnol. 38:615-618.
33. Simon, R., U. Priefer, and A. Puhler. 1983. A broad host range mobilisation system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1:784-791.
34. Swift, S., A. V. Karlyshev, L. Fish, E. L. Durant, M. K. Winson, S. R. Chhabra, P. Williams, S. MacIntyre, and G. A. A. B. Stewart. 1997. Quorum sensing in Aeromonas hydrophila and Aeromonas salmonicida: identification of the LuxRI homologues AhyRI and AsaRI and their cognate N-acylhomoserine lactone signal molecules. J. Bacteriol. 179:5271-5281.
35. Trust, T. J., B. Noonan, S. Chu, P. Lutwyche, and E. Umelo. 1996. Molecular approaches to understanding the pathogenesis of Aeromonas salmonicida - relevance to vaccine development. Fish Shellfish Immunol. 6:269-276.
36. Vaughan, L. M., P. R. Smith and T. J. Foster. 1993. An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis. Infect. Immun. 61:2172-2181.
37. Wardlaw, A. C. 1985. Practical statistics for experimental biologists. John Wiley & Sons, Chichester, United Kingdom.