Methyl green-pyronine staining of porcine organotypic skin explant cultures: An alternative model for screening for skin irritants

ATLA Alternatives to Laboratory Animals

Volumn 28, Issue 2, 2000, Pages 279-292

  Jacobs, John J L ^a   Lehé, Cynthia ^b   Cammans, Keith D A ^a   Das, Pranab K ^b   Elliott, Graham R ^a  

^a TNO   (Netherlands)

^b NONE

Author keywords

Alternative method; Irritant testing; Methyl green pyronine; Pig skin

Indexed keywords

IRRITANT AGENT; METHYL GREEN; PYRONINE;

ANIMAL TISSUE; CONFERENCE PAPER; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; SCREENING TEST; SKIN CULTURE; SKIN IRRITATION; SKIN TOXICITY; SWINE; TOXICITY TESTING;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 0034112412     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290002800206     Document Type: Conference Paper

References (42)

1. Anon. (1988). Commission European Communities Collaborative study on relationship between in vivo primary irritation and in vitro experimental models. EC Report.
2. Draize, J.H., Woodard, G. & Calvery, H.O. (1944). Methods for the study of irritation and toxicity substances applied topically to the skin and mucous membrane. Journal of Pharmacological and Experimental Therapy 82, 377-390.
3. van de Sandt, J.J.M., Rutten, A.A.J.J.L. & Koëter, H.B.W.M. (1993). Cutaneous toxicity testing in organ culture: neutral red uptake and reduction of tetrazolium salt. Toxicology in Vitro 7, 81-86.
4. van de Sandt, J.J.M., van Schoonhoven, J., Maas, W.J.M. & Kutten, A.A.J.J.L. (1993). Skin organ culture as an alternative to in vivo dermatotoxicity testing. ATLA 21, 443-449.
5. Botham, P.A., Earl, L.K., Fentem, J.H., Roguet, R. & van de Sandt, J.J.M., (1998). Alternative methods for skin irritation testing: the current status. ECVAM Skin Irritation Task Force Report 1. ATLA 26, 195-211.
6. Hoh, A., Maier, K. & Dreher, R.M. (1987-88). Multilayered keratinocyte culture used for in vitro toxicology. Molecular Toxicologv 1, 537-546.
7. Triglia, D., Braa, S.S., Yonan, C. & Naughton, G.K. (1991). In vitro toxicity of various classes of test agents using the neutral red assay on a human three-dimensional physiological skin model. In Vitro Cellular and Development Biology: Animal 27, 239-244.
8. Osborne, R. & Perkins, M.A. (1994). An approach for development of alternative test methods based on mechanisms of skin irritation. Food and Chemical Toxicology 32, 133-142.
9. Osborne, R., Perkins, M.A. & Robberts, D.A. (1995). Development and intralaboratory evaluation of an in vitro human cell-based test to aid ocular irritancy assessments. Fundamental and Applied Toxicology 28, 139-153.
10. Brosin, A., Wolf, V., Mattheus, A. & Heise, H. (1997). Use of XTT-assay to assess the cytotoxicity of different surfactants and metal salts in human keratinocytes (HaCaT). A feasible method for in vitro testing of skin irritants. Acta Dermatology and Venereology 77, 26-28.
11. 2 -an in vitro human skin model: the correlation between in vivo and in vitro testing of surfactants. Experimental Dermatology 7, 18-26.
12. Augustin, C. & Damour, O. (1995). Pharmacotoxicological applications of an equivalent dermis: three measurements of cytotoxicity. Cell Biology and Toxicology 11, 167-171.
13. Grangsjö, A., Leijon-Kuligowski, A., Torma, H., Roomans, G.M. & Lindberg, M. (1996). Different pathways in irritant contact eczema? Early differences in the epidermal elemental content and expression of cytokines after application of 2 different irritants. Contact Dermatitis 35, 355-360.
14. Bouclier, M., Cavey, D. & Hensby, C. (1990). Experimental models in skin pharmacology. Pharmacological Reviews 42, 128-154.
15. Hoyer, P.E., Lyon, H., Jakobsen, P. & Andersen, A.P. (1986). Standardized methyl green-pyronine Y procedures using pure dyes. Histochemical Journal 18, 90-94.
16. Schulte, E.K.W., Lyon, H.O. & Hoyer, P.E. (1992). Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronine technique with the gallocyanin chromalum and Feulgen procedures using image cytometry. Histochemical Journal 24, 305-310.
17. Moffitt, P. (1994). A methyl green-pyronine technique for demonstrating cell death in the murine tumour S180. Cell Biology International 18, 305-310.
18. Rentrop, M., Knapp, B., Winter, H. & Schweizer, H. (1986). Aminoalkylsilane-treated glass slides as support for in situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions. Histochemical Journal 18, 271-276.
19. EEC (1988). Council Directive of 7 June 1988 on the approximation of the laws, regulations and administrative provisions of the Member States relating to the classification, packaging and labelling of dangerous substances. Official Journal of the European Communities L18, 14.
20. EEC (1991). Annex I to Council Directive 91/325/EEC of 1 March 1991 adapting to technical progress for the twelfth time Council Directive 67/548/EEC on the approximation of the laws, regulations and administrative provisions of the Member States relating to the classification, packaging and labelling of dangerous substances. Official Journal of the European Communities L180, 34.
21. OECD (1992). OECD Guidelines for the Testing of Chemicals, No. 404: Acute Dermal Irritation/Corrosion, 6pp. Paris, France: OECD.
22. Balls, M., Botham, P.A., Bruner, L.H. & Spielman, H. (1995). The EC/HO international validation study on alternatives to the Draize eye irritation test. Toxicology in vitro 9, 871-929.
23. ECETOC (1995). Skin irritation and corrosion: reference chemicals data bank. Brussels, Belgium: European Centre for Ecotoxicology and Toxicology of Chemicals.
24. Judge, M.R., Griffiths, H.A., Basketter, D.A., White, I.R., Rycroft, R.J.G. & McFadden, J.P. (1996). Variation in response of human skin to irritant challenge. Contact Dermatitis 34, 115-117.
25. McFadden, J.P., Wakelin, S.H. & Basketter, D.A. (1998). Acute irritation thresholds in subjects with type I-type VI skin. Contact Dermatitis 38, 147-149.
26. York, M., Griffiths, H.A., Whittle, E. & Basketter, D.A. (1996). Evaluation of a human patch test for the identification and classification of skin irritation potential. Contact Dermatitis 34, 204-212.
27. Robinson, M.K., Perkins, M.A. & Osborne, R. (1997). Comparative studies on cultured human skin models for irritation testing. In Animal Alternatives, Welfare and Ethics (ed. L.F.M. van Zutphen & M. Balls), 1123-1134. Amsterdam, Netherlands: Elsevier.
28. Basketter, D.A. (1998). Chemistry of contact allergens and irritants. American Journal of Contact Dermatitis 9, 119-124.
29. Nickoloff, B.J. (1998). Immunologic reaction triggered during irritant contact dermatitis. American Journal of Contact Dermatitis 9, 107-110.
30. Serup, J. & Staberg, B. (1987). Differentiation of allergic and irritant reactions by transepidermal water loss. Contact Dermatitis 16, 129-132.
31. Perkins, M.A., Osbourne, R. & Johnson, G.R. (1996). Development of an in vitro method for skin corrosion testing. Fundamental and Applied Toxicology 31, 9-18.
32. Groot, A.C.D. & Frosch, P.J. (1997). Adverse reactions to fragrances. Contact Dermatitis 36, 57-86.
33. Wahlberg, J.E. & Maibach, H.I. (1980). Nonanoic acid irritation: a positive control at routine patch testing? Contact Dermatitis 6, 128-130.
34. Krawiec, D.R. & Gaafar, S.M. (1975). A comparative study of allergic and primary irritant contact dermatitis with dinitrochlorobenzene (DNCB) in dogs. Journal of Investigative Dermatology 65, 248-251.
35. Basketter, D.A., Scholes, E.W. & Kimber, I. (1994). The performance of the local lymph node assay with chemicals identified as contact allergens in the human maximization assay. Food and Chemical Toxicology 32, 543-547.
36. Nordlind, K. & Lidén, S. (1992). Patch test reactions to metal salts in patients with oral mucosal lesions associated with amalgam restorations. Contact Dermatitis 27, 157-160.
37. Willis, C.M., Stephens, C.J. & Wilkinson, J.D. (1988). Experimentally-induced irritant contact dermatitis: determination of optimum irritant concentrations. Contact Dermatitis 18, 20-24.
38. Koch, P. & Nickolaus, G. (1996). Allergic contact dermatitis and mercury exanthem due to mercury chloride in plastic boots. Contact Dermatitis 34, 405-409.
39. Laine, J., Kalimo, K. & Happonen, R.P. (1997). Contact allergy to dental restorative materials in patients with oral lichenoid lesions. Contact Dermatitis 36, 141-146.
40. Schnuch, A., Geier, J., Uter, W., Frosch, P.J., Lehmacher, W., Aberer, W., Agathos, M., Arnold, R., Fuchs, T., Laubstein, B., Lischka, G., Pietrzyk, P.M., Rakoski, J., Richter, G. & Ruëff, F. (1997). National rates and regional differences in sensitization to allergens of the standard series. Contact Dermatitis 37, 200-209.
41. Parish, W.E. (1986). Evaluation of in vitro predictive tests for irritation and allergic sensitization. Food and Chemical Toxicology 24, 481-494.
42. Basketter, D.A., Gerberick, F., Kimber, I. & Willis, C (1998). Toxicology of Contact Dermatitis, 180pp. Chichester, UK: John Wiley.