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Volumn 14, Issue 1, 2000, Pages 25-45

The use of site-directed mutagenesis, transient transfection, and radioligand binding: A method for the characterization of receptor-ligand interactions

Author keywords

Amino acid; Calcium phosphate; Heterologous expression; Oligonucleotide; Radioligand binding; Receptor; Site directed mutagenesis; Transient transfection; tsA201 cells

Indexed keywords

ARTICLE; CHEMICAL MODIFICATION; CROSS LINKING; GENETIC TRANSFECTION; LIGAND BINDING; PHOTOAFFINITY LABELING; SITE DIRECTED MUTAGENESIS;

EID: 0034108422     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:14:1:25     Document Type: Article
Times cited : (5)

References (58)
  • 1
    • 0015607082 scopus 로고
    • A simple assay for the study of solubilized acetylcholine receptors
    • 1. Schmidt, J. and Raftery, M.A. ( 1973). A simple assay for the study of solubilized acetylcholine receptors. Anal. Biochem. 52, 349-354.
    • (1973) Anal. Biochem. , vol.52 , pp. 349-354
    • Schmidt, J.1    Raftery, M.A.2
  • 2
    • 0016697198 scopus 로고
    • Properties of γ-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions
    • 2. Enna, S.J. and Snyder, S.H. (1975). Properties of γ-aminobutyric acid (GABA) receptor binding in rat brain synaptic membrane fractions. Brain. Res. 100, 81-97.
    • (1975) Brain. Res. , vol.100 , pp. 81-97
    • Enna, S.J.1    Snyder, S.H.2
  • 3
    • 0001946792 scopus 로고    scopus 로고
    • Characterization of receptors by radiolabelled ligand-binding techniques
    • (Baker, G.B., Boulton, A.A, and Bateson, A.N, eds.), Humana, Totowa, NJ
    • 3. Davies, M. and Dunn, S.M.J. (1999). Characterization of receptors by radiolabelled ligand-binding techniques, in Neuromethods, vol. 34: In Vitro Neurochemical Techniques (Baker, G.B., Boulton, A.A, and Bateson, A.N, eds.), Humana, Totowa, NJ, pp. 1-35.
    • (1999) Neuromethods, Vol. 34: In Vitro Neurochemical Techniques , vol.34 , pp. 1-35
    • Davies, M.1    Dunn, S.M.J.2
  • 5
    • 0022416158 scopus 로고
    • Chemical mechanisms for photoaffinity labeling of receptors
    • 5. Cavalla, D. and Neff, N.H. (1985). Chemical mechanisms for photoaffinity labeling of receptors. Biochem. Pharmacol. 34, 2821-2826.
    • (1985) Biochem. Pharmacol. , vol.34 , pp. 2821-2826
    • Cavalla, D.1    Neff, N.H.2
  • 6
    • 0021255670 scopus 로고
    • Characterization of γ-aminobutyric acid-benzodiazepine receptor complexes by protection against inactivation by group-specific reagents
    • 6. Maksay, G. and Ticku, M.K. (1984). Characterization of γ-aminobutyric acid-benzodiazepine receptor complexes by protection against inactivation by group-specific reagents. J. Neurochem. 42, 1715-1727.
    • (1984) J. Neurochem. , vol.42 , pp. 1715-1727
    • Maksay, G.1    Ticku, M.K.2
  • 7
    • 0022387904 scopus 로고
    • Chemical and biochemical crosslinking of membrane components
    • 7. Gaffney, B.J. (1985). Chemical and biochemical crosslinking of membrane components. Biochim. Biophys. Acta 822, 289-317.
    • (1985) Biochim. Biophys. Acta , vol.822 , pp. 289-317
    • Gaffney, B.J.1
  • 8
    • 0030431744 scopus 로고    scopus 로고
    • Principles of membrane protein assembly and structure
    • 8. von Heijne, G. (1996). Principles of membrane protein assembly and structure. Prog. Biophys. Mol. Biol. 66, 113-139.
    • (1996) Prog. Biophys. Mol. Biol. , vol.66 , pp. 113-139
    • Von Heijne, G.1
  • 11
    • 0018593044 scopus 로고
    • Site-specific mutagenesis using synthetic oligodeoxyribonucleotides primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length
    • 11. Gillam, S. and Smith, M. (1979). Site-specific mutagenesis using synthetic oligodeoxyribonucleotides primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length. Gene 8, 81-97.
    • (1979) Gene , vol.8 , pp. 81-97
    • Gillam, S.1    Smith, M.2
  • 12
    • 0018633792 scopus 로고
    • Site-specific mutagenesis using synthetic oligodeoxyribonucleotides primers: II In vitro selection of mutant DNA
    • 12. Gillam, S. and Smith, M. (1979). Site-specific mutagenesis using synthetic oligodeoxyribonucleotides primers: II In vitro selection of mutant DNA. Gene 8, 99-106.
    • (1979) Gene , vol.8 , pp. 99-106
    • Gillam, S.1    Smith, M.2
  • 13
    • 0019252609 scopus 로고
    • Site-specific mutagenesis using oligodeoxyribonucleotides: Isolation of a phenotypically silent φX174 mutant, with a specific nucleotide deletion, with very high efficiency
    • 13. Gillam, S. and Smith, M. (1980). Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent φX174 mutant, with a specific nucleotide deletion, with very high efficiency. Gene 12, 129-137.
    • (1980) Gene , vol.12 , pp. 129-137
    • Gillam, S.1    Smith, M.2
  • 14
    • 0022292635 scopus 로고
    • In vitro mutagenesis
    • 14. Smith, M. (1985) In vitro mutagenesis. Annu. Rev. Genet. 19, 423-462.
    • (1985) Annu. Rev. Genet. , vol.19 , pp. 423-462
    • Smith, M.1
  • 15
    • 0024346633 scopus 로고
    • Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR)
    • 15. Kammann, M., Laufs, J., Schell, J. and Gronenborn, B. (1989). Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR). Nucleic Acids Res. 17, 5404.
    • (1989) Nucleic Acids Res. , vol.17 , pp. 5404
    • Kammann, M.1    Laufs, J.2    Schell, J.3    Gronenborn, B.4
  • 16
    • 0027192826 scopus 로고
    • Site-specific mutagenesis in plasmids: A gapped circle method
    • 16. Hofer, B. and Kühlein, B. (1993). Site-specific mutagenesis in plasmids: a gapped circle method. Methods Enzymol. 217, 173-189.
    • (1993) Methods Enzymol. , vol.217 , pp. 173-189
    • Hofer, B.1    Kühlein, B.2
  • 17
    • 0021826423 scopus 로고
    • Cassette mutagenesis: An efficient method for generation of multiple mutations at defined sites
    • 17. Wells, J.A., Yasser, M. and Powers, D.B. (1985). Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites. Gene 34, 315-323.
    • (1985) Gene , vol.34 , pp. 315-323
    • Wells, J.A.1    Yasser, M.2    Powers, D.B.3
  • 19
    • 0021672088 scopus 로고
    • Oligonucleotide-directed mutagenesis: A simple method using two oligonucleotide primers and single stranded DNA template
    • 19. Zoller, M.J. and Smith, M. (1984). Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and single stranded DNA template. DNA 3, 479-488.
    • (1984) DNA , vol.3 , pp. 479-488
    • Zoller, M.J.1    Smith, M.2
  • 21
    • 0021101167 scopus 로고
    • Efficient site-directed mutagenesis by simultaneous use of two primers
    • 21. Norris, K., Norris, F., Christiansen, L. and Fiil, N. (1983). Efficient site-directed mutagenesis by simultaneous use of two primers. Nucleic Acids Res. 11, 5103-5112.
    • (1983) Nucleic Acids Res. , vol.11 , pp. 5103-5112
    • Norris, K.1    Norris, F.2    Christiansen, L.3    Fiil, N.4
  • 22
    • 2642699794 scopus 로고
    • Rapid and efficient site specific mutagenesis without phenotypic selection
    • 22. Kunkel, T.A. (1985). Rapid and efficient site specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. 82, 488-492.
    • (1985) Proc. Natl. Acad. Sci. , vol.82 , pp. 488-492
    • Kunkel, T.A.1
  • 23
    • 0025888264 scopus 로고
    • Efficient site-directed mutagenesis using uracil containing DNA
    • 23. Kunkel, T.A., Bebenek, K. and McClary, T. (1991). Efficient site-directed mutagenesis using uracil containing DNA. Methods Enzymol. 204, 125-139.
    • (1991) Methods Enzymol. , vol.204 , pp. 125-139
    • Kunkel, T.A.1    Bebenek, K.2    McClary, T.3
  • 24
    • 0023613953 scopus 로고
    • Rapid and efficient site-specific mutagenesis without phenotypic selection
    • 24. Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987). Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-385.
    • (1987) Methods Enzymol. , vol.154 , pp. 367-385
    • Kunkel, T.A.1    Roberts, J.D.2    Zakour, R.A.3
  • 25
    • 0029716535 scopus 로고    scopus 로고
    • A protocol for site-directed mutagenesis employing a uracil containing phagemid template
    • (Harwood, A., ed.), Humana, Totowa, NJ
    • 25. Trower, M.K. (1996). A protocol for site-directed mutagenesis employing a uracil containing phagemid template, in Methods in Molecular Biology, vol. 58: Basic DNA and RNA Protocols (Harwood, A., ed.), Humana, Totowa, NJ, pp. 469-476.
    • (1996) Methods in Molecular Biology, Vol. 58: Basic DNA and RNA Protocols , vol.58 , pp. 469-476
    • Trower, M.K.1
  • 26
    • 0026458299 scopus 로고
    • A simple and efficient method for the oligodeoxyribonucleotide-directed mutagenesis of double stranded plasmid DNA
    • 26. Jung, R., Scott, M.P., Oliveria, L.O. and Nielsen, N.C. (1992). A simple and efficient method for the oligodeoxyribonucleotide-directed mutagenesis of double stranded plasmid DNA. Gene 121, 17-24.
    • (1992) Gene , vol.121 , pp. 17-24
    • Jung, R.1    Scott, M.P.2    Oliveria, L.O.3    Nielsen, N.C.4
  • 28
    • 0031114314 scopus 로고    scopus 로고
    • Site-directed mutagenesis using positive antibiotic selection
    • 28. Bohnsack, R.N. (1997). Site-directed mutagenesis using positive antibiotic selection. Mol. Biotech. 7, 181-188.
    • (1997) Mol. Biotech. , vol.7 , pp. 181-188
    • Bohnsack, R.N.1
  • 29
    • 0025300970 scopus 로고
    • Efficient site-directed in vitro mutagenesis using ampicillin selection
    • 29. Lewis, M.K. and Thompson, D.V. (1990). Efficient site-directed in vitro mutagenesis using ampicillin selection. Nucleic Acids Res. 18, 3439-3443.
    • (1990) Nucleic Acids Res. , vol.18 , pp. 3439-3443
    • Lewis, M.K.1    Thompson, D.V.2
  • 30
    • 0028226932 scopus 로고
    • Oligonuclcotide design and optimized protocol for site-directed mutagenesis
    • 30. Piechoki, M.P. and Hines, R. (1994). Oligonuclcotide design and optimized protocol for site-directed mutagenesis. Biotechniques 16, 702-707.
    • (1994) Biotechniques , vol.16 , pp. 702-707
    • Piechoki, M.P.1    Hines, R.2
  • 31
    • 0000163138 scopus 로고
    • Site-directed mutagenesis of cloned DNA
    • Cold Spring Harbour Press, New York, NY
    • 31. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Site-directed mutagenesis of cloned DNA, in Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Press, New York, NY, pp. 15.51-15.85.
    • (1989) Molecular Cloning: A Laboratory Manual , pp. 1551-1585
    • Sambrook, J.1    Fritsch, E.F.2    Maniatis, T.3
  • 32
    • 0021237247 scopus 로고
    • On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying φX174 DNA in vitro
    • 32. Kunkel, T.A., Loeb, L.A. and Goodman, M.F. (1984). On the fidelity of DNA replication. The accuracy of T4 DNA polymerases in copying φX174 DNA in vitro. J. Biol. Chem. 259, 1539-1545.
    • (1984) J. Biol. Chem. , vol.259 , pp. 1539-1545
    • Kunkel, T.A.1    Loeb, L.A.2    Goodman, M.F.3
  • 33
    • 0000748795 scopus 로고    scopus 로고
    • Introduction of DNA into mammalian cells
    • (Bensonchanda, V. ed.), John Wiley and Sons, Boston, MA.
    • 33. Kingston, R. (1997). Introduction of DNA into mammalian cells, in Current Protocols in Molecular Biology (Bensonchanda, V. ed.), John Wiley and Sons, Boston, MA. pp. 9.0.1-9.5.0.
    • (1997) Current Protocols in Molecular Biology , pp. 901-950
    • Kingston, R.1
  • 36
    • 0023392945 scopus 로고
    • High efficiency transformation of mammalian cells by plasmid DNA
    • 36. Chen, C.A. and Okayama, H. (1987). High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745-2752.
    • (1987) Mol. Cell. Biol. , vol.7 , pp. 2745-2752
    • Chen, C.A.1    Okayama, H.2
  • 37
    • 0006500895 scopus 로고
    • Mechanisms of DNA uptake by mammalian cells: Fate of exogenously added DNA monitored by fluorescent dyes
    • 37. Loyter, A., Scangos, G.A. and Ruddle, F.H. (1982). Mechanisms of DNA uptake by mammalian cells: Fate of exogenously added DNA monitored by fluorescent dyes. Proc. Natl. Acad. Sci. 79, 422-426.
    • (1982) Proc. Natl. Acad. Sci. , vol.79 , pp. 422-426
    • Loyter, A.1    Scangos, G.A.2    Ruddle, F.H.3
  • 38
    • 0023711798 scopus 로고
    • Calcium phosphate-mediated gene transfer: A highly efficient transfection system for stably transforming cells with plasmid DNA
    • 38. Chen, C.A. and Okayama, H. (1988). Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA. Biotechniques 6, 632-638.
    • (1988) Biotechniques , vol.6 , pp. 632-638
    • Chen, C.A.1    Okayama, H.2
  • 39
    • 0027474962 scopus 로고
    • Importance of DNA quality for transfection efficiency
    • 39. Ehlert, F., Bierbaum, P. and Schorr, J. (1993). Importance of DNA quality for transfection efficiency. Biotechniques 14, 546.
    • (1993) Biotechniques , vol.14 , pp. 546
    • Ehlert, F.1    Bierbaum, P.2    Schorr, J.3
  • 40
    • 0029991350 scopus 로고    scopus 로고
    • Transfecting mammalian cells: Optimization of critical parameters affecting calcium-phosphate precipitate formation
    • 40. Jordan, M., Sehallhorn, A. and Wurm, F.M. (1996). Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res. 24, 596-601.
    • (1996) Nucleic Acids Res. , vol.24 , pp. 596-601
    • Jordan, M.1    Sehallhorn, A.2    Wurm, F.M.3
  • 41
    • 0031106818 scopus 로고    scopus 로고
    • Addition of glycerol during DNA exposure enhances calcium-phosphate transfection
    • 41. Wilson, S.P. and Smith, L.A. (1996). Addition of glycerol during DNA exposure enhances calcium-phosphate transfection. Anal. Biochem. 246, 148-150.
    • (1996) Anal. Biochem. , vol.246 , pp. 148-150
    • Wilson, S.P.1    Smith, L.A.2
  • 44
    • 0003089394 scopus 로고
    • Neurotransmitter, hormone or drug-receptor binding models
    • (Yamamura, H., ed.), Raven, New York, NY
    • 44. Bennett, J.P. Jr. and Yamamura, H. (1985). Neurotransmitter, hormone or drug-receptor binding models, in Neurotransmitter Receptor Binding (Yamamura, H., ed.), Raven, New York, NY, pp. 61-89.
    • (1985) Neurotransmitter Receptor Binding , pp. 61-89
    • Bennett J.P., Jr.1    Yamamura, H.2
  • 45
    • 0021342186 scopus 로고
    • Receptor binding studies
    • 45. Rottenberg, H., Waring, A. and Rubin, E. (1984). Receptor binding studies. Science 223, 191-193.
    • (1984) Science , vol.223 , pp. 191-193
    • Rottenberg, H.1    Waring, A.2    Rubin, E.3
  • 47
    • 0024473881 scopus 로고
    • A receptor in bovine brain membranes
    • A receptor in bovine brain membranes. Biochemistry 28, 4200-4208.
    • (1989) Biochemistry , vol.28 , pp. 4200-4208
    • Agey, M.W.1    Dunn, S.M.J.2
  • 50
    • 0000648512 scopus 로고    scopus 로고
    • Introduction of plasmid DNA into cells
    • (Bensonchanda, V., ed.), John Wiley and Sons, Boston, MA
    • 50. Seidman, C.E., Struhl, K., Sheen, J. and Jessen, T. (1997). Introduction of plasmid DNA into cells, in Current Protocols in Molecular Biology (Bensonchanda, V., ed.), John Wiley and Sons, Boston, MA, pp. 1.8.2-1.8.6.
    • (1997) Current Protocols in Molecular Biology , pp. 182-186
    • Seidman, C.E.1    Struhl, K.2    Sheen, J.3    Jessen, T.4
  • 51
    • 0023084783 scopus 로고
    • Analysis of mutations in human cells by using an Epstein-Barr, virus shuttle system
    • 51. DuBridge, R.B., Tang, P., Hsia, H.C., Leong, P.M., Miller, J.H. and Calos, M.P. (1987). Analysis of mutations in human cells by using an Epstein-Barr, virus shuttle system. Mol. Cell. Biol. 7, 379-381.
    • (1987) Mol. Cell. Biol. , vol.7 , pp. 379-381
    • Dubridge, R.B.1    Tang, P.2    Hsia, H.C.3    Leong, P.M.4    Miller, J.H.5    Calos, M.P.6
  • 52
    • 0013664002 scopus 로고
    • Use of competent cells for DNA transformations
    • 52. Jessee, J. (1984) Use of competent cells for DNA transformations. Focus 6, 5.
    • (1984) Focus , vol.6 , pp. 5
    • Jessee, J.1
  • 55
    • 0021722488 scopus 로고
    • Rapid-filtration technique for membrane fragments or immobilized enzymes: Measurements of substrate binding or ion fluxes with a few-millisecond time resolution
    • 55. Dupont, Y. (1984). Rapid-filtration technique for membrane fragments or immobilized enzymes: measurements of substrate binding or ion fluxes with a few-millisecond time resolution. Anal. Biochem. 142, 504-510.
    • (1984) Anal. Biochem. , vol.142 , pp. 504-510
    • Dupont, Y.1
  • 56
    • 0003742071 scopus 로고
    • Hybridization with nucleic acid probes part 1 : Theory and nucleic acid preparation
    • (van der Vliet, P.C., ed.), Eisevier, New York, NY
    • 56. Tijssen, P. (1993). Hybridization with nucleic acid probes part 1 : theory and nucleic acid preparation, in Laboratory Techniques in Biochemistry and Molecular Biology (van der Vliet, P.C., ed.), Eisevier, New York, NY, pp. 19-78.
    • (1993) Laboratory Techniques in Biochemistry and Molecular Biology , pp. 19-78
    • Tijssen, P.1
  • 57
    • 0031159128 scopus 로고    scopus 로고
    • The quantitative analysis of multiple mRNA species using oligonucleotide probes in an S1 nuclease protection assay
    • 57. Tanay, V.A.-M.I., Tancowny, B., Glencorse, T.A. and Bateson, A.N. (1997). The quantitative analysis of multiple mRNA species using oligonucleotide probes in an S1 nuclease protection assay. Mol. Biotech. 7, 217-229.
    • (1997) Mol. Biotech. , vol.7 , pp. 217-229
    • Tanay, V.A.-M.I.1    Tancowny, B.2    Glencorse, T.A.3    Bateson, A.N.4
  • 58
    • 0006205921 scopus 로고    scopus 로고
    • Enzymatic manipulation of DNA and RNA
    • (Bensonchanda, V. ed.), John Wiley and Sons, Boston, MA
    • 58. Struhl, K. (1997). Enzymatic manipulation of DNA and RNA, in Carrent Protocols in Molecular Biology (Bensonchanda, V. ed.), John Wiley and Sons, Boston, MA, pp. 3.1.1-3.1.6.
    • (1997) Carrent Protocols in Molecular Biology , pp. 311-316
    • Struhl, K.1


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