Site-Directed Mutagenesis Using Positive Antibiotic Selection

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 7, Issue 2, 1997, Pages 181-188

  Bohnsack, Richard N ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

In vitro mutagenesis; Positive antibiotic selection; Site directed mutagenesis

Indexed keywords

ESCHERICHIA COLI;

ANTIINFECTIVE AGENT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM TRANSFORMATION; DNA DENATURATION; DNA TEMPLATE; ESCHERICHIA COLI; GENE VECTOR; GENETICS; PENICILLIN RESISTANCE; SITE DIRECTED MUTAGENESIS;

AMPICILLIN RESISTANCE; ANTI-BACTERIAL AGENTS; ESCHERICHIA COLI; GENETIC VECTORS; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID DENATURATION; TEMPLATES, GENETIC; TETRACYCLINE RESISTANCE; TRANSFORMATION, BACTERIAL;

EID: 0031114314     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02761754     Document Type: Article

References (15)

1. Smith, M. (1985) In vitro mutagenesis. Ann. Rev. Genet. 19, 423-462.
2. Hutchinson, C. A., Phillips, S., Edgell, M. H., Gillam, S., Jahnke, P., and Smith, M. (1978) Mutagenesis at a specific position in a DNA sequence. J. Biol. Chem. 253, 6551-6559.
3. Wu, R. and Grossman, L. (1987) Site-specific mutagenesis and protein engineering, Subheading IV, Chapters 17-20. Methods Enzymol. 154, 329-403.
4. Kunkel, T. A. (1985) Rapid and efficient site-specific mutagenesis without phenotype selection. Proc. Natl. Acad. Sci. USA 82, 488-492.
5. Lewis, K. and Thompson, D. V. (1990) Efficient site directed in vitro mutagenesis using ampicillin selection. Nucleic Acids Res. 18, 3439-3443.
6. Kramer, B., Kramer, W., and Fritz, H. J. (1990) Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. Coli. Cell 38, 879-887.
7. Siegel, E. C., Wain, S. L., Meltzer, S. F., Binion, M. L., and Steinberg, J. L. (1982) Mutator mutations in Escherichia coli induced by the insertion of phage mu and the transposable resistance elements Tn5 and Tn10. Mutat. Res. 93, 25-33.
8. Zell, R. and Fritz, H. J. (1987) DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues. EMBO J. 6, 1809-1815.
9. Dotto, G. P., Enea, V., and Zinder, N. D. (1981) Functional analysis of bacteriophage f1 intergenic region. Virology 114, 463-473.
10. Dotto, G. P. and Zinder, N. D. (1983) The morphogenetic signal of bacteriophage f1. Virology 130, 252-256.
11. Dotto, G. P., Horiuchi, K., and Zinder, N. D. (1984) The functional origin of bacteriophage f1 DNA replication. Its signals and domains. J. Mol. Biol. 172, 507-521.
12. Altered Sites II in vitro Mutagenesis Systems Manual, #TM001 (1994) Promega Corp., Madison, WI.
13. Piechocki, M. P. and Hines, R. N. (1994) Oligonucleotide design and optimized protocol for site-directed mutagenesis. BioTechniques 16, 702-707.
14. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Hanahan, D. (1985) Techniques for transformation of E. coli, in DNA Cloning, vol 1 (Glover, G. M., ed.), IRL Press, Oxford, UK.