Genotype-based screen for ENU-induced mutations in mouse embryonic stem cells

Nature Genetics

Volumn 24, Issue 3, 2000, Pages 314-317

  Chen, Yijing ^a   Yee, Della ^a   Dains, Katherine ^b   Chatterjee, Aurobindo ^b   Cavalcoli, James ^b   Schneider, Elizabeth ^a   Om, Jinsop ^a   Woychik, Richard P ^b   Magnuson, Terry ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ETHYLNITROSOUREA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EMBRYO; GENE ACTIVITY; GENE FREQUENCY; GENE MUTATION; GENETIC ANALYSIS; GENETIC PROCEDURES; GENETIC SCREENING; GENOTYPE; MUTATION RATE; NONHUMAN; PRIORITY JOURNAL; STEM CELL;

AMINO ACID SUBSTITUTION; ANIMALS; CHIMERA; CODON; DNA DAMAGE; DNA REPAIR; ETHYLNITROSOUREA; GENOTYPE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MICE; MICE, INBRED C57BL; MUTAGENESIS; MUTAGENS; MUTATION; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; POINT MUTATION; RNA SPLICING; STEM CELLS;

ANIMALIA; MAMMALIA; RODENTIA;

EID: 0034091810     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/73557     Document Type: Article

References (23)

1. Justice, M.J., Noveroske, J.K., Weber, J.S., Zheng, B. & Bradley, A. Mouse ENU mutagenesis. Hum. Mol. Genet. 8, 1955-1963 (1999).
2. Russell, W.L. et al. Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proc. Natl Acad. Sci. USA 76, 5818-5819 (1979).
3. Hitotsumachi, S., Carpenter, D.A. & Russell, W.L. Dose-repetition increases the mutagenic effectiveness of N-ethyl-N-nitrosourea in mouse spermatogonia. Proc. Natl Acad. Sci. USA 82, 6619-6621 (1985).
4. Favor, J., Neuhauser-Klaus, A. & Ehling, U.H. The induction of forward and reverse specific-locus mutations and dominant cataract mutations in spermatogonia of treated strain DBA/2 mice by ethylnitrosourea. Mutat. Res. 249, 293-300 (1991).
5. Kasarskis, A., Manova, K. & Anderson, K.V. A phenotype-based screen for embryonic lethal mutations in the mouse. Proc. Natl Acad. Sci. USA 95, 7485-7490 (1998).
6. Shedlovsky, A., McDonald, J.D., Symula, D. & Dove, W.F. Mouse models of human phenylketonuria. Genetics 134, 1205-1210 (1993).
7. Vitaterna, M.H. et al. Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior. Science 264, 719-725 (1994).
8. Justice, M.J. & Bode, V.C. Induction of new mutations in a mouse t-haplotype using ethylnitrosourea mutagenesis. Genet Res. 47, 187-192 (1986).
9. Rinchik, E.M. & Carpenter, D.A. N-ethyl-N-nitrosourea mutagenesis of a 6- to 11-cM subregion of the Fah-Hbb interval of mouse chromosome 7: completed testing of 4557 gametes and deletion mapping and complementation analysis of 31 mutations. Genetics 152, 373-383 (1999).
10. Shedlovsky, A., King, T.R. & Dove, W.F. Saturation germ line mutagenesis of the murine t region including a lethal allele at the quaking locus. Proc. Natl Acad. Sci. USA 85, 180-184 (1988).
11. Jansen, J.G., van Teijlingen, C.M., Mohn, G.R., van Zeeland, A.A. & Vrieling, H. AT base pairs are the main target for mutations at the Hprt locus of rat skin fibroblasts exposed in vitro to the monofunctional alkylating agent N-ethyl-N-nitrosourea. Mutagenesis 9, 417-421 (1994).
12. Douglas, G.R., Jiao, J., Gingerich, J.D., Gossen, J.A. & Soper, L.M. Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice. Proc. Natl Acad. Sci. USA 92, 7485-7489 (1995).
13. Munroe, R.J. et al. Mouse mutants from chemically mutagenized embryonic stem cells. Nature Genet. 24, 318-321 (2000).
14. 6-alkylguanine. FASEB J. 6, 2302-2310 (1992).
15. 6-alkylguanine alkyltransferase. Environ. Mol. Mutagen. 29, 168-179 (1997).
16. Brown, S.D. & Nolan, P.M. Mouse mutagenesis-systematic studies of mammalian gene function. Hum. Mol. Genet. 7, 1627-1633 (1998).
17. Hrabe de Angelis, M. & Balling, S. Large scale ENU screens in the mouse: genetics meets genomics. Mutat. Res. 400, 25-32 (1998).
18. Russell, LB., Russell, W.L., Rinchik, E.M. & Hunsicker, P.R. Factors affecting the nature of induced mutations. in Banbury Report. Biology of Mammalian Germ Cell Mutagenesis 271-285 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1990).
19. Bronstein, S.M., Cochrane, J.E., Craft, T.R., Swenberg, J.A. & Skopek, T.R. Toxicity, mutagenicity, and mutational spectra of N-ethyl-N-nitrosourea in human cell lines with different DNA repair phenotypes. Cancer Res. 51, 5188-5197 (1991).
20. Op het Veld, C.W., van Hees-Stuivenberg, S., van Zeeland, A.A. & Jansen, J.G. Effect of nucleotide excision repair on Hprt gene mutations in rodent cells exposed to DNA ethylating agents. Mutagenesis 12, 417-424 (1997).
21. Threadgill, D.W., Yee, D., Matin, A., Nadeau, J.H. & Magnuson, T. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm. Genome 8, 390-393 (1997).
22. 6-alkylguanine-DNA alkyltransferase activity based on cellular DNA content in human, rat and mouse tissues. Carcinogenesis 7, 745-749 (1986).
23. Konecki, D.S., Brennand, J., Fuscoe, J.C., Caskey, C.T. & Chinault, A.C. Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. Nucleic Acids Res. 10, 6763-6775 (1982).