Rapid nonradioactive in-situ hybridization for mRNA detection of calcineurin in rat brain using PCR DNA probe

Acta Histochemica et Cytochemica

Volumn 33, Issue 2, 2000, Pages 115-122

  Matsui, Toshifumi ^a   Usuda, Nobuteru ^b   Arai, Hiroyuki ^a   Nakazawa, Ayami ^b   Matsushita, Sachio ^c   Higuchi, Susumu ^c   Sasaki, Hidetada ^a  

^a TOHOKU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c KURIHAMA NATIONAL HOSPITAL   (Japan)

Author keywords

Albumin; Alpha fetoprotein (AFP); Calcineurin; Digoxigenin; DNA probe; In situ hybridization; Polymerase chain reaction (PCR)

Indexed keywords

ALBUMIN; ALPHA FETOPROTEIN; BRAIN TISSUE; CALCINEURIN; DIGOXIGENIN 11 DEOXYURIDINE 5' TRIPHOSPHATE; DNA PROBE; IN SITU HYBRIDIZATION; LIVER; MESSENGER RNA; NUCLEIC ACID DETECTION; POLYMERASE CHAIN REACTION; PROTEIN SUBUNIT; RAT; TISSUE CULTURE;

EID: 0034082790     PISSN: 00445991     EISSN: None     Source Type: Journal    
DOI: 10.1267/ahc.33.115     Document Type: Article

References (28)

1. Abelev, G. I.: Alpha-fetoprotein as a marker of embryo-specific differentiations in normal and tumor tissues. Transplant Rev. 20; 3-37, 1974.
2. Chomczynski, P. and Sacchi, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162; 156-159, 1987.
3. Cox, K., DeLeon, D., Angeren, L. and Angeren, R.: Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101; 485-502, 1984.
4. Feinberg, P. and Vogelstein, B.: A technique for radiolabeling DNA restriction enzyme fragments to high specific activity. J. Mol. Biol. 137; 266-267, 1983.
5. Gebeyehu, G., Rao, P. Y., Soochan, P. L., Simms, D. A. and Klevan, L.: Novel biotinylated nucleotide-analogs for labelling and colorimetric detection of DNA. Nucl. Acids Res. 11; 4513-4534, 1987.
6. Gehlert, D. R., Chronwall, B. M., Schafer, M. P. and O'Donohue, T. L.: Localization of neuropeptide Y messenger ribonucleic acid in rat and mouse brain by in situ hybridization. Synapse 1; 25-31, 1987.
7. Giri, P. R., Marietta, C. A., Higuchi, S. and Kincaid, R. L.: Molecular and phylogenetic analysis of calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit genes. DNA Cell Biol. 11; 415-424, 1992.
8. Hayashi, S., Gillam, I. C., Delaney, A. D. and Tener, G. M.: Acetylation of chromosome squashes of drosophila melanogaster decreases the background in autoradiographs from hydridization with [125I]-labeled RNA. J. Histochem. Cytochem. 26; 677-679, 1978.
9. Höltke, H. J. and Kessler, C.: Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection. Nucl. Acids Res. 18; 5843-5851, 1988.
10. Ito, A., Hashimoto, T., Hirai, M., Takeda, T., Shuntoh, H., Kuno, T. and Tanaka, C.: The complete primary structure of calcineurin A, a calmodulin binding protein homologous with protein phosphatase 1 and 2A. Biochem. Biophys. Res. Commun. 163; 1492-1497, 1989.
11. Jacobsson, B.: In situ localization of transthyretin-mRNA in the adult human liver, choroid plexus and pancreatic islets and in endocrine tumors of the pancreas and gut. Histochemistry 91; 299-304, 1989.
12. Jagodzinski, L. L., Sargent, T. D., Yang, M., Glackin, C. and Bonner, J.: Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin. Proc. Natl. Acad. Sci. USA 78; 3521-3525, 1981.
13. Kashimoto, H., Kitazawa, R., Maeda, S., Mizuno, K. and Kitazawa, S.: Modulation of osteonectin and osteopontin expression during fracture healing of mouse bone tissue. Acta Histochem. Cytochem. 31; 501-508, 1998.
14. Klee, C. B., Crouch, T. H. and Krinks, M. H.: Calcineurin: a calcium- and calmodulin-binding protein of the nervous system. Proc. Natl. Acad. Sci. USA 76; 6270-6273, 1979.
15. Koji, T., Chedid, M., Rubin, J. S., Slayden, O. D., Csaky, K. G., Aaronson, S. A. and Brenner, R. M.: Progesterone-dependent expression of keratinocyte growth factor mRNA in stromal cells of the primate endometrium: keratinocyte growth factor as a progestomedin. J. Cell Biol. 125; 393-401, 1994.
16. Kuno, T., Takeda, T., Hirai, M., Ito, A., Mukai, H. and Tanaka, C.: Evidence for a second isoform of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin A). Biochem. Biophys. Res. Commun. 165; 1352-1358, 1989.
17. Lion, T. and Haas, O. A.: Nonradioactive labelling of probe with digoxigenin by polymerase chain reaction. Anal. Biochem. 188; 335-337, 1990.
18. Moorman, A. F., De Boer, P. A., Evans, D., Charles, R. and Lamers, W. H.: Expression patterns of mRNAs for alpha-fetoprotein and albumin in the developing rat: the ontogenesis of hepatocyte heterogeneity. Histochem. J. 22; 653-660, 1990.
19. Nakano, A., Morikawa, S. and Harada, T.: Ubiquitous expression of bcl-2 protein in mitotic nuclei and chromosomes. Acta Histochem. Cytochem. 31; 475-483, 1998.
20. Rigby, P. W. J., Dieckmann, M., Rhodes, C. and Berg, P.: Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113; 237-251, 1977.
21. Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B. and Erlich, H. A.: Analysis of enzymatically amplified b-globin and HLA-Dqa DNA with allele-specific oligonucleotide probes. Nature 324; 163-166, 1986.
22. Seibl, R., Höltke, H. J., Rüger, R., Meindl, A., Zachau, H. G., Rabhofer, G., Roggendorf, W., Wolf, H., Arnold, N., Wienberg, J. and Kessler, C.: Non-radioactive labeling and detection of nucleic acids. III. Applications of the digoxigenin system. Biol. Chem. Hoppe. Seyler 371; 939-951, 1990.
23. Sell, S., Longley, M. A. and Boulter, J.: Alpha-fetoprotein and albumin gene expression in brain and other tissues of fetal and adult rats. Brain Res. 354; 49-53, 1985.
24. Takaishi, T., Saito, N., Kuno, T. and Tanaka, C.: Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain. Biochem. Biophys. Res. Commun. 174; 393-398, 1991.
25. Templeton, N. S.: The polymerase chain reaction. History, methods, and applications. Diagn. Mol. Pathol. 1; 58-72, 1992.
26. Tilghman, S. M. and Belavew, M.: Transcriptional control of the murine albumin/α-fetoprotein locus during development. Proc. Natl. Acad. Sci. USA 79; 5254-5257, 1982.
27. Usuda, N., Arai, H., Sasaki, H., Hanai, T., Nagata, T., Muramatsu, T., Kincaid, R. L. and Higuchi, S.: Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin) in central nervous system neurons: immunohistochemistry on formalin-fixed paraffin sections employing antigen retrieval by microwave irradiation. J. Histochem. Cytochem. 44; 13-18, 1996.
28. Zischler, H., Nanda, I., Schafer, R., Schmid, M. and Epplen, J. T.: Digoxigenated oligonucleotide probes specific for simple repeats in DNA fingerprinting and in situ hybridization. Hum. Genet. 82; 227-233, 1989.