Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin) in central nervous system neurons: Immunohistochemistry on formalin-fixed paraffin sections employing antigen retrieval by microwave irradiation

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 1, 1996, Pages 13-18

  Usuda, Nobuteru ^a   Arai, Hiroyuki ^b   Sasaki, Hidetada ^b   Hanai, Toru ^a   Nagata, Tetsuji ^a   Muramatsu, Taro ^c   Kincaid, Randall L ^d   Higuchi, Susumu ^c,e  

^a SHINSHU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b TOHOKU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c Department of Psychiatry   (Japan)

^d VERITAS Inc   (United States)

^e KURIHAMA NATIONAL HOSPITAL   (Japan)

Author keywords

Calcineurin, Neurons; Catalytic subunit; Isoforms; Microwave irradiation, Immunohistochemistry

Indexed keywords

MAMMALIA; ANIMALIA; BOS TAURUS;

ANTIGEN; CALCINEURIN; FORMALDEHYDE; ISOENZYME; PARAFFIN;

ADULT; AGED; ANIMAL TISSUE; ARTICLE; CATTLE; CELLULAR DISTRIBUTION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; ENZYME ACTIVE SITE; EVOLUTION; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MALE; MICROWAVE IRRADIATION; NONHUMAN; PRIORITY JOURNAL; PURKINJE CELL; PYRAMIDAL NERVE CELL; RAT; TISSUE FIXATION; TISSUE SECTION;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS; CALCINEURIN; CALMODULIN-BINDING PROTEINS; CENTRAL NERVOUS SYSTEM; FORMALDEHYDE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MICROWAVES; MOLECULAR SEQUENCE DATA; PARAFFIN EMBEDDING; PHOSPHOPROTEIN PHOSPHATASE; RATS; RATS, WISTAR; TISSUE FIXATION;

EID: 0030068408     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.1.8543776     Document Type: Article

References (31)

1. Arai H, Lee V, Otvos L, Greenberg BD, Lowery DE, Sharma SK, Schmitt ML, Trojanowski JQ: Defined neurofilament, τ, and β-amyloid precursor protein epitopes distinguish Alzheimer from non-Alzheimer senile plaques Proc Natl Acad Sci USA 87.2249, 1990
2. Bachs O, Lanin L, Serratosa J, Coll MJ, Bastos R, Aligué R, Rius E, Carafoli E: Calmodulin-binding proteins in the nuclei of quiescent and proliferatively activated rat liver cells. J Biol Chem 265:18595, 1990
3. Billingsley ML, Polli JW, Pennypacker KR, Kincaid RL: Identification of calmodulin-binding proteins. Methods Enzymol 194:451, 1990
4. Bosser R, Aligué R, Guerini D, Agell N, Carafoli E, Bachs O: Calmodulin can modulate protein phosphorylation in rat liver cells nuclei. J Biol Chem 268:15477, 1993
5. Flanagan WM, Corthesy B, Bram RJ, Crabtree GR: Nuclear association of a T-cell transcription factor blocked by FK-506 and cyclosporin A. Nature 352:682, 1991
6. Giri PR, Marietta CA, Higuchi S, Kincaid RL: Molecular and phylogenetic analysis of calmodulin-dependent protein phosphatase (calcineurin) catalytic subunit genes. DNA Cell Biol 11:415, 1992
7. Goto S, Matsukado Y, Mihara Y, Inoue N, Miyamoto E: The distribution of calcineurin in rat brain by light and electron microscopic immunohistochemistry and enzyme-immunoassay. Brain Res 397:161, 1986
8. Goto S, Matsukado Y, Mihara Y, Inoue N, Miyamoto E: Calcineurin as a neuronal marker of human brain tumors. Brain Res 371:237, 1986
9. Goto S, Nagahiro S, Korematsu K, Ushio Y, Fukunaga K, Miyamoto E, Hofer W: Cellular colocalization of calcium/calmodulin-dependent protein kinase II and calcineurin in rat cerebral cortex and hippocampus Neurosci Lett 149:189, 1993
10. Guerini D, Klee CB: Cloning of human calcineurin A: evidence for two isoforms and identification of a polyproline structural domain. Proc Natl Acad Sci USA 86:9183, 1989
11. Halpain S, Girault JA, Greengard P: Activation of NMDA receptors induces dephosphorylation of DARPP-32 in rat striatal slices. Nature 343-369, 1990
12. Halpain S, Greengard P: Activation of NMDA receptors induces induced rapid dephosphorylation of the cytoskeletal protein MAP2. Cell 5:237, 1990
13. Hsu S, Raine L, Fanger H: Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577, 1981
14. Ito A, Hashimoto T, Hirai M, Takada T, Shuntoh H, Kuno T, Tanaka C: The complete primary structure of calcineurin A, a calmodulin binding protein homologous with protein phosphatases 1 and 2A. Biochem Biophys Res Commun 163:1492, 1989
15. King MM, Hunag CY, Chock PB, Nair AC, Hemmings HC, Jesse Chan K-F, Greengard P: Mammalian brain phosphoprotein as substrates for calcineurin. J Biol Chem 259:8080, 1984
16. Klee CB: Concerted regulation of protein phosphorylation and dephosphorylation by calmodulin. Neurochem Res 16.1059, 1991
17. Klee CB, Crouch TH, Krinks MH: Calcineurin: a calcium- and calmodulin-binding protein of the nervous system. Proc Natl Acad Sci USA 76:6270, 1979
18. Kuno T, Takeda T, Hirai M, Ito A, Mukai H, Tanaka C: Evidence of a second isoform of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin). Biochem Biophys Res Commun 165:1352, 1989
19. McCaffrey PG, Perrino BA, Soderling TR, Rao A: NF-ATp, a T lymphocyte DNA-binding protein that is a target for calcineurin and immunosuppresive drugs. J Biol Chem 268:3747, 1993
20. McKeon F: When worlds collide: immunosuppressants meet protein phosphatases. Cell 66:823, 1991
21. Muramatsu T, Giri PR, Higuchi S, Kincaid RL: Molecular cloning of a calmodulin-dependent protein phosphatase from murine testis: identification of a developmentally expressed nonneural isozyme. Proc Natl Acad Sci USA 89:529, 1992
22. Muramatsu T, Kincaid RL: Molecular cloning of a full-length cDNA encoding the catalytic subunit of human calmodulin-dependent protein phosphatase (calcineurin Aα). Biochim Biophys Acta 1178:117, 1993
23. Pujol MJ, Bosser R, Vendrell M, Serratosa J, Bachs O: Nuclear calmodulin-binding proteins in rat neurons. J Neurochem 60:1422, 1993
24. 2+-calmodulin-dependent protein kinase in neuronal nuclei. J Biol Chem 259:9341, 1984
25. Schreiber SL: Immunophilin-sensitive protein phosphatase action in cell signaling pathways. Cell 70:365, 1992
26. Shi S-R, Chaiwun B, Young L, Cote RJ, Taylor CR: Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections. J Histochem Cytochem 41:1599, 1993
27. Shi S-R, Key ME, Kalra KL: Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741, 1991
28. Shimizu T, Usuda N, Yamanda T, Sugenoya A, Lida F: Proliferative activity of human thyroid tumors evaluated by proliferating cell nuclear antigen/cyclin immunohistochemical studies Cancer 71:2807, 1993
29. Soderling TR: Protein kinases. J Biol Chem 265:1823, 1990
30. Usuda N, Kong Y, Hagiwara M, Uchida C, Terasawa M, Nagata T, Hidaka H: Differential localization of protein kinase C isozymes in retinal neurons. J Cell Biol 112:1241, 1990
31. Yokoyama N, Furuyama S, Wang JH: Demonstration of calmodulin-stimulated phosphatase isozymes by monoclonal antibodies. J Biol Chem 265:8170, 1990