Topography of diphtheria toxin's T domain in the open channel state

Journal of General Physiology

Volumn 115, Issue 4, 2000, Pages 421-434

  Senzel, Lisa ^a   Gordon, Michael ^a   Blaustein, Robert O ^b   Oh, K Joon ^c   Collier, R John ^c   Finkelstein, Alan ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b BRANDEIS UNIVERSITY   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Histidine tag; Planar bilayers; Streptavidin; Translocation

Indexed keywords

DIPHTHERIA TOXIN; HISTIDINE; ION CHANNEL; PEPTIDE; STREPTAVIDIN;

ARTICLE; BILAYER MEMBRANE; BIOTINYLATION; CHANNEL GATING; MEMBRANE CONDUCTANCE; MOLECULAR MODEL; NONHUMAN; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; TOPOGRAPHY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BIOTIN; CELL MEMBRANE; CYSTEINE; DIPHTHERIA TOXIN; HUMANS; ION CHANNEL GATING; ION CHANNELS; LIPID BILAYERS; MALEIMIDES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; STREPTAVIDIN;

EID: 0034081629     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.115.4.421     Document Type: Article

References (27)

1. Bennett, M.J., S. Choe, and D. Eisenberg. 1994. Refined structure of dimeric diphtheria toxin at 2.0. Å resolution. Prot. Sci. 3:1444-1463.
2. Choe, S., M.J. Bennett, G. Fujii, P.M.G. Curmi, K.A. Kantardjieff, R.J. Collier, and D. Eisenberg. 1992. The crystal structure of diphtheria toxin. Nature. 357:216-222.
3. Donovan, J.J., M.I. Simon, R.K. Draper, and M. Montal. 1981. Diphtheria toxin forms transmembrane channels in planar lipid bilayers. Proc. Natl. Acad. Sci. USA. 78:172-176.
4. Draper, R.K., and M.I. Simon. 1980. The entry of diphtheria toxin into the mammalian cell cytoplasm: evidence for lysosomal involvement. J. Cell Biol. 87:849-854.
5. Eriksen, S., S. Olsnes, K. Sandvig, and O. Sand. 1994. Diphtheria toxin at low pH depolarizes the membrane, increases the membrane conductance and induces a new type of ion channel in Vero cells. EMBO (Eur. Mol. Biol. Organ.) J. 13:4433-4439.
6. Falnes, P.O., I.H. Madshus, K. Sandvig, and S. Olsnes. 1992. Replacement of negative by positive charges in the presumed membrane-inserted part of diphtheria toxin B fragment: effect on membrane translocation and on formation of channels. J. Biol. Chem. 267:12284-12290.
7. Greenfield, L., M.J. Bjorn, G. Horn, D. Fong, G.A. Buck, R.J. Collier, and D.A. Kaplan. 1983. Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage b. Proc. Natl. Acad. Sci. USA. 80:6853-6857.
8. Huynh, P., C. Cui, H. Zhan, K.J. Oh, R.J. Collier, and A. Finkelstein. 1997. Probing the structure of the diphtheria toxin channel: reactivity in planar lipid bilayer membranes of cysteine-substituted mutant channels with methanethiosulfonate derivatives. J. Gen. Physiol. 110:229-242.
9. Kachel, K., J. Ren, R.J. Collier, and E. London. 1998. Identifying transmembrane states and defining the membrane insertion boundaries of hydrophobic helices in membrane-inserted diphtheria toxin T domain. J. Biol. Chem. 273:22950-22956.
10. Kagan, B.L., A. Finkelstein, and M. Colombini. 1981. Diphtheria toxin fragment forms large pores in phospholipid bilayer membranes. Proc. Natl. Acad. Sci. USA. 78:4950-4954.
11. Lanzrein, M., P.O. Falnes, O. Sand, and S. Olsnes. 1997. Structure-function relationship of the ion channel formed by diphtheria toxin in Vero cell membranes. J. Membr. Biol. 156:141-148.
12. Madshus, I., and H. Stenmark. 1992. Entry of ADP-ribosylating toxins into cells. Curr. Top. Microbiol. Immunol. 175:1-26.
13. Mindell, J.A., J.A. Silverman, R.J. Collier, and A. Finkelstein. 1994a. Structure-function relationships in diphtheria toxin channels. II. A residue responsible for the channel's dependence on trans pH. J. Membr. Biol. 137:29-44.
14. Mindell, J.A., J.A. Silverman, R.J. Collier, and A. Finkelstein. 1994b. Structure-function relationships in diphtheria toxin channels: III. Residues which affect the cis pH dependence of channel conductance. J. Membr. Biol. 137:45-57.
15. Moskaug, J., H. Stenmark, and S. Olsnes. 1991. Insertion of diphtheria toxin B-fragment into the plasma membrane at low pH. J. Biol. Chem. 266:2652-2659.
16. Neilsen, O., and O. Buchardt. 1991. Facile synthesis of reagents containing a terminal maleimido ligand linked to an active ester. Synthesis. 10:819-821.
17. Oh, K.J., L. Senzel, R.J. Collier, and A. Finkelstein. 1999. Translocation of the catalytic domain of diphtheria toxin across planar phospholipid bilayers by its own T domain. Proc. Natl. Acad. Sci. USA. 96:8467-8470.
18. Oh, K.J., H. Zhan, C. Cui, K. Hideg, R.J. Collier, and W.L. Hubbell. 1996. Organization of diphtheria toxin T domain in bilayers: a site-directed spin labeling study. Science. 273:810-812.
19. Qiu, X.-Q., K.S. Jakes, A. Finkelstein, and S.L. Slatin. 1994. Site-specific biotinylation of colicin Ia: a probe for protein conformation in the membrane. J. Biol. Chem. 269:7483-7488.
20. Qiu, X.-Q., K.S. Jakes, P.K. Kienker, A. Finkelstein, and S.L. Slatin. 1996. Major transmembrane movement associated with colicin Ia channel gating. J. Gen. Physiol. 107:313-328.
21. Quertenmont, P., R. Wattiez, P. Falmagne, J.M. Ruysschaert, and V. Cabiaux. 1996. Topology of diphtheria toxin in lipid vesicle membranes: a proteolysis study. Mol. Microbiol. 21:1283-1296.
22. Sandvig, K., and S. Olsnes. 1980. Diphtheria toxin entry into cells is facilitated by low pH. J. Cell Biol. 87:828-832.
23. 2-terminal region across planar bilayers. J. Gen. Physiol. 112:317-324.
24. Silverman, J.A., J.A. Mindell, H. Zhan, A. Finkelstein, and R.J. Collier. 1994a. Structure-function relationships in diphtheria toxin channels: determining a minimal channel-forming domain. J. Membr. Biol. 137:17-28.
25. Silverman, J.A., J.A. Mindell, A. Finkelstein, W.H. Shen, and R.J. Collier. 1994b. Mutational analysis of the helical hairpin region of diphtheria toxin transmembrane domain. J. Biol. Chem. 269: 22524-22532.
26. Wang, Y., K. Kachel, L. Pablo, and E. London. 1997. Use of Trp mutations to evaluate the conformational behavior and membrane insertion of A and B chains in whole diphtheria toxin. Biochemistry. 36:16300-16308.
27. Zhan, H., K.J. Oh, Y.K. Shin, W.L. Hubbell, and R.J. Collier. 1995. Interaction of the isolated transmembrane domain of diphtheria toxin with membranes. Biochemistry. 34:4856-4863.