Sequencing difficult DNA templates using membrane-mediated loading with hot sample application

BioTechniques

Volumn 28, Issue 4, 2000, Pages 628-630

  Gerstner, Arpad ^a   Sasvari Szekely, Maria ^a   Kalasz, Huba ^a   Guttman, Andras ^a,b  

^a SEMMELWEIS UNIVERSITY OF MEDICINE   (Hungary)

^b SYNGENTA BIOTECHNOLOGY INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; SINGLE STRANDED DNA;

ARTICLE; DENATURATION; DNA DETERMINATION; DNA SEQUENCE; DNA TEMPLATE; ELECTRIC FIELD; HYDROGEN BOND; SEQUENCE ANALYSIS;

BACTERIOPHAGE M13; DNA, SINGLE-STRANDED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEAT; MEMBRANES, ARTIFICIAL; SEQUENCE ANALYSIS, DNA; TEMPLATES, GENETIC;

EID: 0034081243     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (7)

1. Cassel, S.M. and A. Guttman. 1998. Membrane-mediated sample loading for automated DNA sequencing. Electrophoresis 19:1341-1346.
2. Guttman, A., C. Barta, M. Szoke, M. Sasvari-Szekely and H. Kalasz. 1998. Real-time detection of allele-specific polymerase chain reaction products by automated ultra-thin-layer agarose gel electrophoresis. J. Chromatogr. A 828:481-487.
3. Griffin, H.G. and A.M. Griffin. (Eds.) 1993. DNA Sequencing Protocols: Methods in Molecular Biology, Vol 23, Humana Press, Totowa, NJ.
4. Mizusawa S., S. Nishimura and F. Seela. 1986. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Res. 14:1319-1324.
5. Ruiz-Martinez, M.C., J. Berka, A. Belenkii, F. Foret, A.W. Miller and B.L. Karger. 1993. DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection. Anal. Chem. 65:2851-2858.
6. Tabor, S. and C.C. Richardson. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84:4767-4771.
7. Yamakawa, H., D. Nakajima and O. Ohara. 1996. Identification of sequence motifs causing band compressions on human cDNA sequencing. DNA Res. 3:81-86.