Single cell analysis of cytokine expression kinetics by human CD4+ T-cell clones during activation or tolerance induction

Immunology

Volumn 100, Issue 2, 2000, Pages 209-216

  Pala, P ^a   Verhoef, A ^b   Lamb, J R ^b,c   Openshaw, P J M ^a  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 4; INTERLEUKIN 5; IONOMYCIN; PHORBOL 13 ACETATE 12 MYRISTATE; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CELL CLONE; CELL PROLIFERATION; CONTROLLED STUDY; CYTOKINE RELEASE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOLOGICAL TOLERANCE; IMMUNOSTIMULATION; PRIORITY JOURNAL; T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION;

ANTIGENS; CD4-POSITIVE T-LYMPHOCYTES; CELL CULTURE TECHNIQUES; CELL DIVISION; CLONE CELLS; CYTOKINES; DOSE-RESPONSE RELATIONSHIP, IMMUNOLOGIC; DOWN-REGULATION; HUMANS; IMMUNE TOLERANCE; INTERFERON TYPE II; INTERLEUKIN-4; INTERLEUKIN-5; LYMPHOCYTE ACTIVATION;

EID: 0034079888     PISSN: 00192805     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2567.2000.00036.x     Document Type: Article

References (17)

1. 1. LAMB J.R., SKIDMORE B.J., GREEN N., CHILLER J.M. & FELDMANN M. (1983) Induction of tolerance in influenza virus-immune T lymphocyte clones with synthetic peptides of influenza hemagglutinin. J Exp Med 157, 1434.
2. 2. RYAN K.R. & EVAVOLD B.D. (1998) Persistence of peptide-induced CD4+ T cell anergy in vitro. J Exp Med 187, 89.
3. 3. SLOAN LANCASTER J., EVAVOLD B.D. & ALLEN P.M. (1993) Induction of T-cell anergy by altered T-cell-receptor ligand on live antigen-presenting cells. Nature (London) 363, 156.
4. 4. TSITOURA D.C., HOLTER W., CERWENKA A., GELDER C.M. & LAMB J.R. (1996) Induction of anergy in human T helper 0 cells by stimulation with altered T cell antigen receptor ligands. J Immunol 156, 2801.
5. 5. O'HEHIR R.E. & LAMB J.R. (1990) Induction of specific clonal anergy in human T lymphocytes by Staphylococcus aureus enterotoxins. Proc Natl Acad Sci USA 87, 8884.
6. 6. WILLIAMS M.E., SHEA C.M., LICHTMAN A.H. & ABBAS A.K. (1992) Antigen receptor-mediated anergy in resting T lymphocytes and T cell clones. Correlation with lymphokine secretion patterns. J Immunol 149, 1921.
7. 7. MADRENAS J., CHAU L.A., SMITH J., BLUESTONE J.A. & GERMAIN R.N. (1997) The efficiency of CD4 recruitment to ligand-engaged TCR controls the agonist/partial agonist properties of peptide-MHC molecule ligands. J Exp Med 185, 219.
8. 8. YSSEL H., FASLER S., LAMB J. & DE VRIES J.E. (1994) Induction of non-responsiveness in human allergen-specific type 2 T helper cells. Curr Opin Immunol 6, 847.
9. 9. O'HEHIR R.E., YSSEL H., VERMA S., DE VRIES J.E., SPITS H. & LAMB J.R. (1991) Clonal analysis of differential lymphokine production in peptide and superantigen induced T cell anergy. Int Immunol 3, 819.
10. 10. FASLER S., AVERSA G., TERR A., THESTRUP PEDERSEN K., DE VRIES J.E. & YSSEL H. (1995) Peptide-induced anergy in allergen-specific human Th2 cells results in lack of cytokine production and B cell help for IgE synthesis. Reversal by IL-2, not by IL-4 or IL-13. J Immunol 155, 4199.
11. 11. SCHALL T.J., O'HEHIR R.E., GOEDDEL D.V. & LAMB J.R. (1992) Uncoupling of cytokine mRNA expression and protein secretion during the induction phase of T cell anergy. J Immunol 148, 381.
12. 12. VERHOEF A., HIGGINS J.A., THORPE C.J. et al. (1993) Clonal analysis of the atopic immune response to the group 2 allergen of Dermatophagoides spp. identification of HLA-DR and DQ restricted T cell epitopes. Int Immunol 5, 1589.
13. 13. LAMB J.R., ECKELS D.D., LAKE P., WOODY J.N. & GREEN N. (1982) Human T-cell clones recognize chemically synthesized peptides of influenza haemagglutinin. Nature (London) 300, 66.
14. 14. EMILIE D., PEUCHMAUR M., BARAD M. et al. (1989) Visualizing interleukin 2 gene expression at the single cell level. Eur J Immunol 19, 1619.
15. 15. OPENSHAW P., MURPHY E.E., HOSKEN N.A. et al. (1995) Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med 182, 1357.
16. 16. ITOH Y. & GERMAIN R.N. (1997) Single cell analysis reveals regulated hierarchical T cell antigen receptor signaling thresholds and intraclonal heterogeneity for individual cytokine responses of CD4+ T cells. J Exp Med 186, 757.
17. H2-like phenotype. J Exp Med 179, 481.