Cloning and expression of UDP-glucose: Flavonoid 7-O-glucosyltransferase from hairy root cultures of Scutellaria baicalensis

Planta

Volumn 210, Issue 6, 2000, Pages 1006-1013

  Hirotani, Masao ^a   Kuroda, Ryo ^a   Suzuki, Hideyuki ^a   Yoshikawa, Takafumi ^a  

^a KITASATO UNIVERSITY   (Japan)

Author keywords

Baicalein; Baicalin; Flavonoid 7 O glucosyltransferase gene; Hairy root culture; Lamiaceae; Scutellaria flavonoids

Indexed keywords

7 O GLUCOSYL TRANSFERASE; AMINO ACID SEQUENCE; BAICALEIN; CATALYSIS; CDNA LIBRARY; ENZYME ACTIVITY; FLAVONOID; MESSENGER RNA; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; RECOMBINANT ENZYME; SALICYLIC ACID; URIDINE DIPHOSPHATE GLUCOSE; WOUND;

AMINO ACID SEQUENCE; BLOTTING, NORTHERN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; ESCHERICHIA COLI; FLAVONOIDS; GLUCOSYLTRANSFERASES; LAMIACEAE; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANT ROOTS; RECOMBINANT PROTEINS;

EID: 0034079353     PISSN: 00320935     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004250050710     Document Type: Article

References (37)

1. Brugliera F, Holton TA, Stevenson TW, Farcy E, Lu C-Y, Cornish EC (1994) Isolation and characterization of a cDNA clone corresponding to the Rt locus of Petunia hybrida. Plant J 5: 81-92
2. Chiang Su New Medical College (ed) (1977) Dictionary of Chinese Crude Drugs, Shanghai Scientific Technological Publishers. Shanghai, pp 2017-2021
3. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159
4. Ford CM, Boss PK, Høj PB (1998) Cloning and characterization of Vitis vinifera UDP-glucose: flavonoid 3-O-glucosyltransferase. a homologue of the enzyme encoded by the maize Bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo. J Biol Chem 273: 9224-9233
5. Fraissinet-Tachet L, Baltz R, Chong J, Kauffmann S, Fritig B, Saindrenan P (1998) Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyltransferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid. FEBS Lett 437: 319-323
6. Furtek D, Schiefelbein JW, Johnston F, Nelson OE Jr (1988) Sequence comparisons of three wild-type Bronze-1 alleles from Zea mays. Plant Mol Biol 11: 473-481
7. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158
8. Gubler U, Hoffman B J (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263-269
9. Heller W, Forkmann G (1994) Biosynthesis of flavonoids. In: Harborne JB (ed) The flavonoids. Advances in research since 1986. Chapman & Hall, London, pp 499-535
10. Hirotani M, Nagshima S, Yoshikawa T (1998) Baicalin and baicalein productions of cultured Scutellaria baicalensis cells. Nat Med 52: 440-443
11. Horvath DM, Chua N-H (1996) Identification of an immediate-early salicylic acid-inducible tobacco gene and characterization of induction by other compounds. Plant Mol Biol 31: 1061-1072
12. Hundle BS, O'Brien DA, Alberti M, Beyer P, Hearst JE (1992) Functional expression of zeaxanthin glucosyltransferase from Erwinia herbicola and a proposed uridine diphosphate binding site. Proc Natl Acad Sci USA 89: 9321-9325
13. Kroon J, Souer E, de Graaff A, Xue Y, Mol J, Koes R (1994) Cloning and structural analysis of the anthocyanin pigmentation locus Rt of Petunia hybrida: characterization of insertion sequences in two mutant alleles. Plant J 5: 69-80
14. 1H nuclear magnetic resonance spectroscopy of flavonoids and their glycosides in hexadeuterodimethylsulfoxide. In: Harborne JB (ed) The flavonoids. Advances in research since 1986. Chapman & Hall, London, pp 441-497
15. Martin C, Prescott A, Mackay S, Bartlett J, Vrijlandt E (1991) Control of anthocyanin biosynthesis in flowers of Antirrhinum majus. Plant J 1: 37-49
16. Mato M, Ozeki Y, Itoh Y, Higeta D, Yoshitama K, Teramoto S, Aida R, Ishikura N, Shibata M (1998) Isolation and characterization of a cDNA clone of UDP-galactose: flavonoid 3-O-galactosyltransferase (UF3GaT) expressed in Vigna mungo seedlings. Plant Cell Physiol 39: 1145-1155
17. McIntosh CA, Mansell RL (1990) Biosynthesis of naringin in Citrus paradisi: UDP-glucosyltransferase activity in grapefruit seedlings. Phytochemistry 29: 1533-1538
18. McIntosh CA, Latchinian L, Mansell RL (1990) Flavanone-specific 7-O-glucosyltransferase activity in Citrus paradisi seedlings: purification and characterization. Arch Biochem Biophys 282: 50-57
19. O'Donnell PJ, Truesdale MR, Calvert CM, Dorans A, Roberts MR, Bowles DJ (1998) A novel tomato gene that rapidly responds to wound- and pathogen-related signals. Plant J 14: 137-142
20. Poulton JE (1990) Cyanogenesis in plants. Plant Physiol 94: 401-405
21. Ralston EJ, English JJ, Dooner HK (1988) Sequence of three bronze alleles of maize and correlation with the genetics fine structure. Genetics 119: 185-197
22. Reay PF, Conn EE (1974) The purification and properties of a uridine diphosphate glucose: aldehyde cyanohydrin β-glucosyl-transferase from sorghum seedlings. J Biol Chem 249: 5826-5830
23. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenic trees. Mol Biol Evol 4: 406-425
24. Sambrook J, Fritsch EF, Maniatis T(1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, NY
25. Schulz M, Weissenböck G (1988) Three specific UDP-glucuronate: flavone-glucuronosyltransferases from primary leaves of Secale cereale. Phytochemistry 27: 1261-1267
26. Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C (1994) Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L). Plant Mol Biol 24: 743-755
27. Stapleton A, Allen PV, Tao HP, Belknap WR, Friedman M (1992) Partial amino acid sequence of potato solanidine UDP-glucose glucosyltransferase purified by new anion-exchange and size exclusion media. Prot Express Purifie 3: 85-92
28. Stich K, Halbwirth H, Wurst F, Forkmann G (1997) UDP-glucose: flavonol 7-O-glucosyltransferase activity in flower extracts of Chrysanthemum segetum. Z Naturforsch 52c: 153-158
29. 3-glucosyltransferase from illuminated red cabbage (Brassica oleracea cv red danish) seedlings. Plant Physiol 95: 570-576
30. Takaiwa F, Oono K, Sugiura M (1985) Nucleotide sequence of the 17S-25 S spacer region from rice rDNA. Plant Mol Biol 4: 355-364
31. Tanaka Y, Yonekura K, Fukuchi-Mizutani M, Fukui Y, Fujiwara H, Ashikari T, Kusumi T (1996) Molecular and biochemical characterization of three anthocyanin synthetic enzymes from Gentiana triflora. Plant Cell Physiol 37: 711-716
32. Tang W, Eisenbrand G. (1992) Chinese drugs of plant origin. Springer, Berlin Heidelberg, pp 919-929.
33. Tomimori T, Miyaichi Y, Imoto Y, Kizu H, Tanabe Y (1984) Studies on the constituents of Scutellaria speceis. III. On the flavonid constituents of the root of Scuterallia baicalensis Georgi(3). Yakugaku Zasshi 104: 524-528
34. Vellekoop P, Lugones L, van Brederode J (1993) Purification of an UDP-glucose: flavone, 7-O-glucosyltransferase, from Silene latifolia using a specific interaction between the enzyme and phenyl-sepharose. FEBS Lett 330: 36-40
35. Wise RP, Rohde W, Salamini F (1990) Nucleotide sequence of the Bronze-I homologous gene from Hordeum vulgare. Plant Mol Biol 14: 277-279
36. Yamazaki M, Gong Z, Fukuchi-Mizutani M, Fukui Y, Tanaka Y, Kusumi T, Saito K (1999) Molecular cloning and biochemical characterization of a novel anthocyanin 5-O-glucosyltransferase by mRNA differential display for plant forms regarding anthocyanin. J Biol Chem 274: 7405-7411
37. Zhou Y, Hirotani M, Yoshikawa T, Furuya T (1997) Flavonoids and phenylethanoids from hairy root cultures of Scutellaria haicalensis. Phytochemistry 44: 83-87