A two-component regulatory system, pehR-pehS, controls endopolygalacturonase production and virulence in the plant pathogen Erwinia carotovora subsp. carotovora

Molecular Plant-Microbe Interactions

Volumn 13, Issue 4, 2000, Pages 447-455

  Flego, Diana ^a   Marits, Reet ^b   Eriksson, Anders R B ^a   Kõiv, Viia ^b   Karlsson, Maj Brit ^a   Heikinheimo, Riikka ^a,d   Palva, E Tapio ^c  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b UNIVERSITY OF TARTU   (Estonia)

^c Biotechnology Institute   (Finland)

^d ASSOCIATION EURATOM TEKES   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME REGULATION; GENE ACTIVATION; GENE LOCUS; GENE MUTATION; GENE STRUCTURE; GENETIC COMPLEMENTATION; GENETIC REGULATION; GENETIC STRAIN; OPERON; PHENOTYPE; POLYGALACTURONASE; REGULATOR GENE; TRANSCRIPTION REGULATION; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CAROTOVORA; ESCHERICHIA COLI; NICOTIANA TABACUM; PECTOBACTERIUM CAROTOVORUM SUBSP. CAROTOVORUM; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0034066979     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI.2000.13.4.447     Document Type: Article

References (57)

1. Andro, T., Chambost, J.-P., Kotoujansky, A., Cattaneo, J., Bertheau, Y., Barras, F., van Gijsegem, F., and Coleno, A. 1984. Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase. J. Bacteriol. 160:1199-1203.
2. Barras, F., van Gijsegem, F., and Chatterjee, A. K. 1994. Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu. Rev. Phytopathol. 32:201-234.
3. Beaulieu, C., Boccara, M., and Van Gijsegem, F. 1993. Pathogenic behavior of pectinase-defective Erwinia chrysanthemi mutants on different plants. Mol. Plant-Microbe Interact. 6:197-202.
4. Brickman, E., and Beckwith, J. 1975. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and Fi80 transducing phages. J. Mol. Biol. 96:307-316.
5. Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104:541-555.
6. Chatterjee, A., Cui, Y., Liu, Y., Dumenyo, C. K., and Chatterjee, A. K. 1995. Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density-sensing signal, N-(3-oxoheanoyl)-L-homoserine lacton. Appl. Environ. Microbiol. 61: 1959-1967.
7. Collmer, A., and Keen, N. T. 1986. The role of pectic enzymes in plant pathogenesis. Annu. Rev. Phytopathol. 24:383-409.
8. Cui, Y., Chatterjee, A., Liu, Y., Dumenyo, C. K., and Chatterjee, A. K. 1995. Identification of a global represser gene, rsmA, of Erwinia carotovora subsp. carotovora that controls extracellular enzymes, N-(3-oxohexanoyl)-L-homoserine lactone, and pathogenicity in soft-rotting Erwinia spp. J. Bacteriol. 177:5108-5115.
9. Eriksson, A. R. B., Andersson, R. A., Pirhonen, M., and Palva, E. T. 1998. Two-component regulators involved in the global control of virulence in Erwinia carotovora subsp. carotovora. Mol. Plant-Microbe Interact. 11:743-752.
10. Fellay, R., Frey, J., and Krisch, H. 1987. Interposon mutagenesis of soil and water bacteria: A family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52:147-154.
11. Fields, P. I., Groisman, E. A., and Heffron, F. 1989. A Salmonella locus that controls resistance to microbicidal proteins from phagocytic cells. Science 243:1059-1062.
12. Fields, P. I., Swanson, R. V., Haidaris, C. G., and Heffron, F. 1986. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proc. Natl. Acad. Sci. USA 83:5189-5193.
13. Flego, D., Pirhonen, M., Saarilahti, H., Palva, T. K., and Palva, E. T. 1997. Control of virulence gene expression by plant calcium in the phytopathogen Erwinia carotovora. Mol. Microbiol. 25:831-838.
14. García-Véscovi, E., Soncini, F. C., and Groisman, E. A. 1994. The role of the PhoP/PhoQ regulon in Salmonella virulence. Res. Microbiol. 145:473-480.
15. 2+ as an extracellular signal: Environmental regulation of Salmonella virulence. Cell 84: 165-174.
16. Govantes, F., Andújar, E., and Santero, E. 1998. Mechanism of translational coupling in the nifLA operon of Klebsiella pneumoniae. EMBO J. 17:2368-2377.
17. Groisman, E. A., Heffron, F., and Solomon, F. 1992. Molecular genetic analysis of the Escherichia coli phoP locus. J. Bacteriol. 174:486-491.
18. Gunn, J. S., Hohmann, E. L., and Miller, S. I. 1996. Transcriptional regulation of Salmonella virulence: A PhoQ periplasmic domain mutation results in increased net phosphotransfer to PhoP. J. Bacteriol. 178:6369-6373.
19. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
20. Heikinheimo, R., Flego, D., Pirhonen, M., Karlsson, M.-B., Eriksson, A., Mäe, A., Kõiv, V., and Palva, E. T. 1995. Characterization of a novel pectate lyase from Erwinia carotovora subsp. carotovora. Mol. Plant-Microbe Interact. 8:207-217.
21. Hess, J. F., Bourret, R. B., and Simon, M. I. 1988. Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis. Nature 336:139-143.
22. Igo, M. M., Ninfa, A. J., Stock, J. B., and Silhavy, T. J. 1989. Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor. Genes Dev. 3:1725-1734.
23. Ji, J., Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. 1987. Use of Mu-lac insertions to study the secretion of pectate lyases by Erwinia chrysanthemi. J. Gen. Microbiol. 133:793-802.
24. Jones, S., Yu, B., Bainton, N. J., Birdsall, M., Bycroft, B. W., Chhabra, S. R., Cox, A. J. R., Golby, P., Reeves, P. J., Stephans, S., Winson, M. K., Salmond, G. P. C., Stewart, G. S. A. B., and Williams, P. 1993. The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J. 12:2477-2482.
25. Kasahara, M., Nakata, A., and Shinagawa, H. 1992. Molecular analysis of the Escherichia coli phoP-phoQ operon. J. Bacteriol. 174:492-498.
26. Kelemu, S., and Collmer, A. 1993. Erwinia chrysanthemi EC16 produces a second set of plant-inducible pectate lyase isozymes. Appl. Environ. Microbiol. 59:1756-1761.
27. Kier, L. D., Weppelman, R. M., and Ames, B. N. 1979. Regulation of nonspecific acid phosphatase in Salmonella: phoN and phoP genes. J. Bacteriol. 138:155-161.
28. Liu, Y., Cui, Y., Mukherjee, A., and Chalterjee, A. K. 1998. Characterization of a novel RNA regulator of Erwinia carotovora ssp. carotovora that controls production of extracellular enzymes and secondary metabolites. Mol. Microbiol. 29:219-234.
29. Liu, Y., Murata, H., Chatterjee, A., and Chatterjee, A. K. 1993. Characterization of a novel regulatory gene aepA that controls extracellular enzyme production in the phytopathogenic bacterium Erwinia carotovora subsp. carotovora. Mol. Plant-Microbe Interact. 6:299-308.
30. Lojkowska, E., Dorel, C., Reignault, P., Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. 1993. Use of GUS fusion to study the expression of Erwinia chrysanthemi pectinase genes during infection of potato tubers. Mol. Plant-Microbe Interact. 6:488-494.
31. Lång, H., and Palva, E. T. 1993. The ompS gene of Vibrio cholerae encodes a growth-phase-dependent maltoporin. Mol. Microbiol. 10:891-901.
32. Mäe, A., Heikinheimo, R., and Palva, E. T. 1995. Structure and regulation of the Erwinia carotovora subspecies carotovora SCC3193 cellulase gene celVI and the role of cellulase in phytopathogenicity. Mol. Gen. Genet. 247:17-26.
33. McCleary, W. R., Stock, J. B., and Ninfa, A. J. 1993. Is acetyl phosphate a global signal in Escherichia coli? J. Bacteriol. 175:2793-2798.
34. Miller, J. F., Mekalanos, J. J., and Falkow, S. 1989. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243:916-922.
35. Miller, J. H. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. p. 466.
36. Miller, S. I., and Mekalanos, J. J. 1990. Constitutive expression of the PhoP regulon attenuates Salmonella virulence and survival within macrophages. J. Bacteriol. 172:2485-2490.
37. Mukherjee, A., Cui, Y., Liu, Y., Dumenyo, C. K., and Chatterjee, A. K. 1996. Global regulation in Erwinia species by Envinia carotovora rsmA, a homologue of Escherichia coli csrA: Repression of secondary metabolites, pathogenicity and hypersensitive reaction. Microbiology 142:427-434.
38. Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
39. Murata, H., Chatterjee, A., Liu, Y., and Chatterjee, A. K. 1994. Regulation of the production of extracellular pectinase, cellulase, and protease in the soft rot bacterium Erwinia carotovora subsp. carotovora: Evidence that aepH of E. carotovora subsp. carotovora 71 activates gene expression in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli. Appl. Environ. Microbiol. 60:3150-3159.
40. Murata, H., McEvoy, J. L., Chatterjee, A., Collmer, A., and Chatterjee, A. K. 1991. Molecular cloning of an aepA gene that activates production of extracellular pectolytic, cellulolytic, and proteolytic enzymes in Erwinia carotovora subsp. carotovora. Mol. Plant-Microbe Interact. 4:239-246.
41. Parkinson, J. S. 1993. Signal transduction schemes of bacteria. Cell 73: 857-871.
42. Parkinson, J. S., and Kofoid, E. C. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
43. Pegues, D. A., Hantman, M. J., Behlau, I., and Miller, S. I. 1995. PhoP/PhoQ transcriptional repression of Salmonella typhimurium invasion genes: Evidence for a role in protein secretion. Mol. Microbiol. 17:169-181.
44. Pérombelon, M. C. M., and Kelman, A. 1980. Ecology of the soft rot Erwinias. Annu. Rev. Phytopathol. 18:361-387.
45. Pirhonen, M., Flego, D., Heikinheimo, R., and Palva, E. T. 1993. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO J. 12:2467-2476.
46. Pirhonen, M., Heino, P., Helander, I., Harju, P., and Palva, E. T. 1988. Bacteriophage T4 resistant mutants of the plant pathogen Erwinia carotovora. Microb. Pathog. 4:359-367.
47. Pirhonen, M., Saarilahti, H., Karlsson, M.-B., and Palva, E. T. 1991. Identification of pathogenicity determinants of Erwinia carotovora subsp. carotovora by transposon mutagenesis. Mol. Plant-Microbe Interact. 4:276-283.
48. Saarilahti, H. T., Heino, P., Pakkanen, R., Kalkkinen, N., Palva, I., and Palva, E. T. 1990. Structural analysis of the pehA gene and characterization of its protein product, endopolygalacturonase, of Erwinia carotovora subspecies carotovora. Mol. Microbiol. 4.1037-1044.
49. Saarilahti, H. T., Pirhonen, M., Karlsson, M.-B., Flego, D., and Palva, E. T. 1992. Expression of pehA-bla gene fusions in Erwinia carotovora subsp. carotovora and isolation of regulatory mutants affecting polygalacturonase production. Mol. Gen. Genet. 234:81-88.
50. Sambrook, J., Fritsch, E. F., and Maniatis, T. A. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
51. Soncini, F. C., García-Véscovi, E., and Groisman, E. A. 1995. Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon. J. Bacteriol. 177:4364-4371.
52. Stock, J. B., Ninfa, A. J., and Stock, A. M. 1989. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol. Rev. 53:450-490.
53. Thurn, K. K., and Chatterjee, A. K. 1985. Single-site chromosomal Tn5 insertions affect the export of pectolytic and cellulolytic enzymes in Erwinia chrysanthemi EC16. Appl. Environ. Microbiol. 50:894-898.
54. Torriani, A. 1966. Alkaline phosphatase from Escherichia coli. Pages 224-235 in: Procedures in Nucleic Acid Research. G. C. Cantoni and R. Davies, eds. Harper & Row, New York.
55. Way, J. C., Davis, M. A., Morisato, D., Roberts, D. E., and Kleckner, N. 1984. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ fusions by transposition. Gene 32:369-379.
56. I) of Escherichia coli. Proc. Natl. Acad. Sci. USA 85:8919-8923.
57. Weppelman, R., Kier, L. D., and Ames, B. N. 1977. Properties of two phosphatases and a cyclic phosphodiesterase of Salmonella typhimurium. J. Bacteriol. 130:411-419.