Directed deletions in the aflatoxin biosynthesis gene homolog cluster of Aspergillus oryzae

Current Genetics

Volumn 37, Issue 2, 2000, Pages 104-111

  Kusumoto, Ken Ichi ^a,b   Nogata, Yoichi ^b   Ohta, Hideaki ^c  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b NATIONAL AGRICULTURAL RESEARCH CENTER FOR WESTERN REGION   (Japan)

^c NAKAMURA GAKUEN UNIVERSITY   (Japan)

Author keywords

Aflatoxin biosynthetic genes; Aspergillus oryzae; Cluster; Deletion

Indexed keywords

AFLATOXIN;

ARTICLE; ASPERGILLUS ORYZAE; ASPERGILLUS PARASITICUS; BIOSYNTHESIS; GENE CLUSTER; GENE DELETION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOUTHERN BLOTTING;

ASPERGILLUS ORYZAE; ASPERGILLUS PARASITICUS;

EID: 0034065860     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940050016     Document Type: Article

References (35)

1. Chang P-K, Bhatnagar D, Cleveland TE, Bennett JW (1995a) Sequence variability in homologs of the aflatoxin pathway gene aflR distinguished species in Aspergillus Section Flavi. Appl Environ Microbiol 61:40-43
2. 1 biosynthesis. Mol Gen Genet 248:270-277
3. Chang P-K, Ehrlich KC, Yu J, Bhatnagar D, Cleveland TE (1995c) Increased expression of Aspergillus parasiticus aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis. Appl Environ Microbiol 61:2372-2377
4. Cole RJ, Cox RH (1981) Handbook of toxic fungal metabolites. Academic Press. New York
5. Geiser DM, Pitt JI, Taylor JW (1998) Cryptic speciation and recombination in the aflatoxin-producinc fungus Aspergillus flavus. Proc Natl Acad Sci USA 95:388-393
6. Gomi K, Tanaka A, Iimura Y, Takahashi K (1989) Rapid differentiation of four related species of koji molds by agarose-gel electrophoresis of genomic DNA digested with the SmaI restriction enzyme. J Gen Appl Microbiol 35:225-232
7. Keller NP, Hohn TM (1997) Metabolic pathway gene clusters in filamentous fungi. Fungal Genet Biol 21:17-29
8. Keller NP, Kantz NJ, Adams TA (1994) Aspergillus nidulans ver A is required for production of the mycotoxin sterigmatocystin. Appl Environ Microbiol 60:1444-1450
9. Klich MA, Yu J, Mullaney EJ (1987) DNA restriction enzyme fragment polymorphism as a tool for rapid differentiation of Aspergillus flavus from Aspergillus oryzae. Exp Mycol 11:170-175
10. Klich MA, Chang P-K, Mullaney EJ, Bhatnagar D, Cleveland TE (1995) Hybridization of genes involved in aflatoxin biosynthesis to DNA of aflatoxigenic and non-aflatoxigenic aspergilli. Appl Microbiol Biotechnol 44:439-443
11. Klich MA, Montalbano B, Ehrlich K (1997) Northern analysis of aflatoxin biosynthesis genes in Aspergillus parasiticus and Aspergillus sojae. Appl Microbiol Biotechnol 47:246-249
12. Kurtzman CP, Smiley MJ, Robnett CJ, Wicklow DJ (1986) DNA relatedness among wild and domesticated species in the Aspergillus flavus group. Mycologia 78:955-959
13. Kusumoto K, Goto T, Manabe M (1990) Evaluation for conversion of sterigmatocystin to aflatoxin in koji-molds. Rep Natl Food Res Inst 54:14-17
14. Kusumoto K, Mori K, Nogata Y, Ohta H, Manabe M (1996) Homologs of an aflatoxin biosynthetic gene ver-1 in the strains of Aspergillus oryzae and its related species. J Ferment Bioeng 82:161-164
15. Kusumoto K, Yabe K, Nogata Y, Ohta H (1998a) Aspergillus oryzae with and without a homolog of the aflatoxin biosynthetic gene ver-1. Appl Microbiol Biotechnol 50:98-104
16. Kusumoto K, Yabe K, Nogata Y, Ohta H (1998b) Transcript of aflR, a regulatory gene for aflatoxin synthesis in Aspergillus parasiticus, was not detected in Aspergillus oryzae strains. FEMS Microbiol Lett 169:303-307
17. Liang S-H, Skory CD, Linz JE (1996) Characterization of the function of the ver-1 A and ver-1B genes, involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol 62:4568-4575
18. Mahanti N, Bhatnagar D, Cary J, Joubran J, Linz JE (1996) Structure and function of a putative fatty acid synthetase directly involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl Environ Microbiol 62:191-195
19. Manabe M, Matsuura S, Nakano M (1968) Studies on the fluorescent compounds in fermented foods Part I. Chloroformsoluble fluorescent compounds produced by Koji-molds. Nippon Shokuhin Kogyogakkaishi 15:341-346
20. Minto RE, Townsend CA (1997) Enzymology and molecular biology of aflatoxin biosynthesis. Chem Rev 97, 2537-2555
21. Murakami H (1971) Taxonomic studies on the Japanese industrial strains of Aspergillus. Part 18. Molds other than the koji mold and mycological characters of all strains. Rep Res Inst Brew Japan 143:16-29
22. Raper KB, Fennell DI (1965) Aspergillus flavus group. In: Raper KB, Fennell DI (eds) The genus Aspergillus. The Williams and Wilkins Co., Baltimore, pp 357-404
23. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
24. Silva JC, Minto RE, Barry III CE, Holland KA, Townsend CA (1996) Isolation and characterization of the versicolorin B synthase gene from Aspergillus parasiticus. J Biol Chem 271: 13600-13608
25. Skory CD, Chang P-K, Cary J, Linz JE (1992) Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin. Appl Environ Microbiol 58:3527-3537
26. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503 517
27. Ushijima S, Nakadai T, Uchida K (1990) Breeding of new kojimolds through interspecific hybridization between Aspergillus oryzae and Aspergillus sojae by protoplast fusion. Agric Biol Chem 54:1667-1676
28. Van der Watt JJ (1977) Sterigmatocystin. In: Purchase IFH (ed) Mycotoxin. Elsevier, New York, pp 368-382
29. Watson AJ, Fuller LJ, Jeenes DJ, Archer DB (1999) Homologs of aflatoxin biosynthesis genes and sequence of aflR in Aspergillus oryzae and Aspergillus sojae. Appl Environ Microbiol 65:307-310
30. Woloshuk CP, Prieto R (1998) Genetic organization and function of the aflatoxin B1 biosynthetic genes. FEMS Microbiol Lett 160:169-176
31. Woloshuk CP, Yousibova GL, Rollins JA, Bhatnagar D, Payne GA (1995) Molecular characterization of the afl-1 locus in Aspergillus flavus. Appl Environ Microbiol 61:3019-3023
32. Yokotsuka T, Sasaki M, Kikuchi T, Asao Y, Nobuhara A (1967) Studies on the compounds produced by moulds. Part 1. Fluorescent compounds produced by Japanese industrial moulds. Nippon Nougeikagaku Kaishi 41:32-38
33. Yu J, Chang P-K, Cary JW, Wright M, Bhatnagar D, Cleveland TE, Payne GA, Linz JE (1995) Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus. Appl Environ Microbiol 61:2365-2371
34. Yu J-H, Butchko RAE, Fernandes M, Keller NP, Leonard TJ, Adams TH (1996) Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus. Curr Genet 29:549-555
35. Yuan G-F, Liu C-S, Chen C-C (1995) Differentiation of Aspergillus parasiticus from Aspergillus sojae by random amplification of polymorphic DNA. Appl Environ Microbiol 61: 2384-2387