Application of confocal laser scanning microscopy to detect oxidative stress in human colon cells

Free Radical Research

Volumn 32, Issue 6, 2000, Pages 535-547

  Liegibel, Ute M ^a   Abrahamse, Salomon L ^b   Pool Zobel, Beatrice L ^a   Rechkemmer, Gerhard ^c  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

^c FEDERAL RESEARCH INSTITUTE OF NUTRITION AND FOOD   (Germany)

Author keywords

6 carboxy 2',7' dichlorodihydrofluorescein diacetate; Comet Assay; Confocal laser scanning microscope; Endonuclease III; Human colon cells; Human colon crypts; Oxidative stress

Indexed keywords

ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CELL STRAIN CACO 2; COLON; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DNA DAMAGE; ELECTROPHORESIS; FLUORESCENCE; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; OXIDATION; OXIDATIVE STRESS;

ANIMALIA;

EID: 0034053747     PISSN: 10715762     EISSN: None     Source Type: Journal    
DOI: 10.1080/10715760000300541     Document Type: Article

References (25)

1. S. Toyokuni, K. Okamoto, J. Yudoi and H. Hiai (1995) Persistent oxidative stress in cancer. FEBS Letters, 358, 1-3.
2. 2 for platelet-derived growth factor signal transduction. Science, 270, 296-299.
3. W.R. Bonorden and M.W. Pariza (1994) Antioxidant nutrients and protection from free radicals. In Nutritional Taxicology (Eds. F.N. Kotsonis, M. Mackey and J. Hielle), Raven Press Ltd, New York pp. 19-48.
4. T. Hirano, Y. Homma and H. Kasai (1995) Formation of 8-hydroxyguanine in DNA by aging and oxidative stress. In Oxidative Stress and Ging (Eds. R.G. Cutler, L. Packer, J. Bertram and A. Mori), Birkhäuser Verlag, Basel pp. 69-76.
5. F.L. Chung, H.J. Candy and R.G. Nath (1996) Lipid peroxidation as a potential endogenous source for the formation of exocyclic DNA adducts. Carcinogenesis, 17, 2105-2111.
6. D. Crawford, I. Zbinden, P. Amstad and P.A. Cerutti (1988) Oxidant stress induces the proto-oncogenes c-fos and c-myc in mouse epidermal cells. Oncogene, 3, 27-32.
7. F. Cimino, F. Esposito, R. Ammendola and T. Russo (1997) Gene regulation by reactive oxygen species. Current Topics in Cellular Regulation, 35, 123-148.
8. M.A. Schwarz, J.S. Lazo, J.C. Yalowich, I. Reynolds, V.E. Kagan, V. Tyurin, Y.M. Kim, S.C. Watkins and B.R. Pitt (1994) Cytoplasmic metallothionein overexpression protects NIH 3T3 cells from tert-butyl hydroperoxide toxicity. Journal of Biological Chemistry, 269, 15238-15243.
9. R.P. Haugland (1996) Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes Europe BV, Leiden, The Netherlands.
10. L. Bhaskar and K.A. Balasubramanian (1996) Luminal exposure of oxidants alter colonic absorptive function. Life Sciences, 58, 2115-2123.
11. A. Kautiainen, T. Midtvedt and M. Törnqvist (1993) Intestinal bacteria and endogenous production of malonaldehyde and alkylators in mice. Carcinogenesis, 14, 2633-2636.
12. O.I. Aruoma (1994) Nutrition and health aspects of free radicals and antioxidants. Food Chemistry and Toxicology, 32, 671-683.
13. G. Cao, E. Sofic and R.L. Prior (1997) Antioxidant and prooxidant behaviour of flavonoids: structure-activity relationships. Free Radical Biology & Medicine, 22, 749-760.
14. M. Rousset (1986) The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation. Biochimic, 68, 1035-1040.
15. C. Augeron and C.L. Laboisse (1984) Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Research, 44, 3961-3969.
16. B.L. Pool-Zobel, S.L. Abrahamse, A.R. Collins, W. Kark, R. Gugler, D. Oberreuther, E.G. Siegel, S. Treptow-van Lishaut and G. Rechkemmer (1999) Analysis of DNA strand breaks, oxidized bases and glutathione S-transferase P1 in human colon cells. Cancer Epidemiology Biomarkers & Prevention, 8, 609-614.
17. N.P. Singh, M.T. McCoy, R.R. Tice and E.L. Schneider (1988) A simple technique for qualification of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184-191.
18. A.R. Collins, S.J. Duthie and V.L. Dobson (1995) Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis, 14, 1733-1735.
19. B.L. Pool-Zobel and L. Leucht (1997) Induction of DNA damage in human colon cells derived from biopsies by suggested risk factors of colon cancer. Mutation Research, 375, 105-116.
20. H. Konishi, G. Steinbach, W.N. Hittelman, K. Fujita, J.J. Lee, G. A. Glober, B. Levin, M. Andreeff, A.M. Good acre and N.H.A. Terry (1996) Cell kinetic analysis of intact rat colonic crypts by confocal microscopy and immunofluorescence. Gastroenterology, 111, 1493-1500.
21. R.A. Brooks and D.J. Winton (1996) Determination of spatial patterns of DNA damage and repair in intestinal crypts by multi-cell gel electrophoresis. Journal of Cell Science, 109, 2061-2068.
22. G. Szakacs, K. Jakab, F. Antal and B. Sarkadi (1998) Diagnostics of multidrug resistance in cancer. Pathology Oncology Research, 4, 251-257.
23. A. Coutois, L. Payen, D. Lagadic, A. Guilouzo and O. Fardel (1999) Evidence for a multidrug resistance-associated protein 1 (MRP1)-related transport system in cultured rat liver biliary epithelium cells. Life Sciences, 64, 763-774.
24. M.P. Draper, R.L. Martell and S.B. Levy (1997) Active efflux of the free acid form of the fluorescent dye 2′,7′-bis(carboxyethyl)-5(6)-carboxyflourescein in multi-drug-resistance-overexpressing murine and human leukemia cells. European Journal of Biochemistry, 243, 219-224.
25. K. Arimori and M. Nakano (1998) Drug exsorption from blood into the gastrointestinal tract. Pharmaceutical Research, 15, 371-376.