Active efflux of the free acid form of the fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein in multidrug-resistance-protein-overexpressing murine and human leukemia cells

European Journal of Biochemistry

Volumn 243, Issue 1-2, 1997, Pages 219-224

  Draper, Michael P ^a   Martell, Robin L ^a   Levy, Stuart B ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

2',7' bis(2 carboxyethyl) 5(6) carboxyfluorescein; Glutathione; Multidrug resistance protein; Murine; P glycoprotein

Indexed keywords

2,7 BIS(CARBOXYETHYL) 6 CARBOXYFLUORESCEIN; BUTHIONINE SULFOXIMINE; DOXORUBICIN; ETOPOSIDE; FLUORESCENT DYE; GLUTATHIONE; GLYCOPROTEIN P; VINCRISTINE;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; DRUG ACCUMULATION; DRUG SENSITIVITY; GENE OVEREXPRESSION; GLUTATHIONE METABOLISM; HUMAN; HUMAN CELL; IN VITRO STUDY; LEUKEMIA CELL LINE; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL;

EID: 0031026289     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.0219a.x     Document Type: Article

References (24)

1. Endicott, J. A. & Ling, V. (1989) The biochemistry of P-glycoprotein-mediated multidrug resistance, Annu. Rev. Biochem. 158, 137-171.
2. Cole, S. P. C., Bhardwaj, G., Gerlach, J. H., Mackie, J. E., Arant, C. E., Almquist, K. C., Stewart, A. J., Kurz, E. U., Duncan, A. M. V. & Deeley, R. G. (1992) Overexpression of a novel transporter gene in a multidrug resistant human lung cancer cell line, Science 258, 1650-1654.
3. Cole, S. P. C., Sparks, K. E., Fraser, K., Loe, D. W., Grant, C. E., Wilson, G. M. & Deeley, R. G. (1994) Pharmacological characterization of multidrug resistant MRP-transfected human tumor cells, Cancer Res. 54, 5902-5910.
4. Draper, M. P., Martell, R. L. & Levy, S. B. (1997) Indomethacin-mediated reversal of multidrug resistance and drug efflux in human and murine cell lines overexpressing MRP, but not P-glycoprotein, BJC, in the press.
5. Jedlitschky, G., Leier, I., Buchholz, U., Center, M. & Keppler, D. (1994) ATP-dependent transport of glutathion, S-conjugates by the multidrug resistance-associated protein, Cancer Res. 5, 4833-4836.
6. 4 and structurally related conjugates, J. Biol. Chem. 269, 27 807-27 810.
7. Jedlitschky, G., Leir, I., Buchholz, U., Barnouin, K., Kurz, G. & Keppler, D. (1996) Transport of glutathione, glucuronate, and sulfate conjugates by the MRP gene-encoded conjugate export pump, Cancer Res. 56, 988-994.
8. Müller, M., Meijer, C., Zaman, G. J. R., Borst, P., Scheper, R. J., Mulder, N. H., de Vries, E. G. E. & Jansen, P. L. M. (1994) Overexpression of the gene encoding the multidrug resistance-associated protein results in increased ATP-dependent glutathione S-conjugate transport, Proc. Natl Acad. Sci. USA 91, 13 033-13 037.
9. Zaman, G. J. R., Flens, M. J., van Leusden, M. R., de Haas, M., Mülder, H. S., Lankelma, J., Pinedo, H. M., Scheper, R. J., Baas, F., Broxterman, H. J. & Borst, P. (1994) The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump, Proc. Natl Acad. Sci. USA 91, 8822-8826.
10. Lorico, A., Rappa, G., Srimatkandada, S., Catapano, C. V., Fernandes, D. J., Germino, J. F. & Sartorelli, A. C. (1995) Increased rate of adenosine triphosphate-dependent etoposide (VP-16) efflux in a murine leukemia cell line overexpressing the multidrug resistance-associated protein (MRP) gene, Cancer Res. 55, 4352-4360.
11. Versantvoort, C. H. M., Broxterman, H. J., Bagrij, T., Scheper, R. J. & Twentyman, P. R. (1995) Regulation by glutathione of drug transport in multidrug-resistant human lung tumour cell lines overexpressing multidrug resistance-associated protein, Br. J. Cancer 72, 82-89.
12. Zaman, G. J. R., Lankelma, J., van Tellingen, O., Beijnen, J., Dekker, H., Paulusma, C., Elferink, R. P. J. O., Baas, F. & Borst, P. (1995) Role of glutathione in the export of compounds from cells by the multidrug-resistance-associated protein, Proc. Natl Acad. Sci. USA 92, 7690-7694.
13. Tew, K. D. (1994) Glutathione-associated enzymes in anticancer drug resistance, Cancer Res. 54, 4313-4320.
14. Homolya, L., Holló, Z., Germann, U. A., Pastan, I., Gottesman, M. M. & Sarkadi, B. (1993) Fluorescent cellular indicators are extruded by the multidrug-resistance protein, J. Biol. Chem. 268, 21 493-21 496.
15. 2+ in lymphocytes, J. Biol. Cell. 95, 189-196.
16. Slapak, C. A., Fracasso, P. M., Martell, R. L., Toppmeyer, D. L., Lecerf, J. M. & Levy, S. B. (1994) Overexpression of the multidrug resistance-associated protein (MRP) gene in vincristine but not doxorubicin-selected multidrug-resistant murine erythroleukemia cells, Cancer Res. 54, 5607-5613.
17. Krishnamachary, N., Ma, L., Zheng, L., Safa, A. R. & Center, M. S. (1994) Analysis of MRP gene expression and function in HL60 cells isolated for resistance to adriamycin, Oncol. Res. 6, 119-127.
18. Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods 65, 55-63.
19. Ellman, G. I. (1959) Tissue sulfhydryl groups, Arch. Biochem. Biophys. 82, 70-77.
20. Slapak, C. A., Martell, R. L., Terashima, M. & Levy, S. B. (1996) Increased efflux of vincristine but not daunorubicin, associated with the murine multidrug-resistance protein (MRP), Biochem. Pharmacol. 52, 1569-1576.
21. Slapak, C. A., Daniel, J. C. & Levy, S. B. (1990) Sequential emergence of distinct resistance phenotypes in murine erythroleukemia cells under adriamycin selection: decreased anthracycline uptake precedes increased P-glycoprotein expression, Cancer Res. 50, 7895-7901.
22. Krishnamachary, N. & Center, M. S. (1993) The MRP gene associated with a non-P-glycoprotein multidrug resistance encodes a 190-kDa membrane bound glycoprotein, Cancer Res. 53, 3658-3661.
23. Tang-Wai, D. F., Kajiji, S., DiCapua, F., de Graaf, D., Roninson, I. B. & Gros, P. (1995) Human (MDR1) and mouse (mdr1, mdr3) P-glycoproteins can be distinguished by their respective drug resistance profiles and sensitivity to modulators, Biochemistry 34, 32-39.
24. Feller, N., Broxterman, H. J., Währer, D. C. R. & Pinedo, H. M. (1995) ATP-dependent efflux of calcein by the multidrug-resistance protein (MRP): no inhibition by intracellular glutathione depletion, FEBS Lett. 368, 385-388.