Evaluation by site-directed mutagenesis of aspartic acid residues in the metal site of pig heart NADP-dependent isocitrate dehydrogenase

Biochemistry

Volumn 39, Issue 9, 2000, Pages 2193-2200

  Grodsky, Neil B ^a   Soundar, Sambanthamurthy ^a   Colman, Roberta F ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; DIVALENT CATION; FERROUS ION; HEART ENZYME; ISOCITRATE DEHYDROGENASE (NADP); MANGANESE;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; METAL BINDING; NONHUMAN; PKA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SWINE;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; FERROUS COMPOUNDS; HYDROLYSIS; IRON; ISOCITRATE DEHYDROGENASE; ISOCITRATES; KINETICS; METALS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MYOCARDIUM; NADP; RECOMBINANT PROTEINS; SWINE;

ESCHERICHIA COLI; SUIDAE; SUS SCROFA;

EID: 0034053460     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9919753     Document Type: Article

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