Expression of pig heart mitochondrial NADP-dependent isocitrate dehydrogenase in Escherichia coli

Protein Expression and Purification

Volumn 8, Issue 3, 1996, Pages 305-312

  Soundar, Sambanthamurthy ^a   Jennings, Gary T ^b   McAlister Henn, Lee ^b   Colman, Roberta F ^a  

^a UNIVERSITY OF DELAWARE   (United States)

^b UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030294869     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0105     Document Type: Article

References (27)

1. 1. Bacon, C. R., Bednar, R. A., and Colman, R. F. (1981) The reaction of 4-iodoacetamidosalicylic acid with TPN-dependent isocitrate dehydrogenase from pig heart. J. Biol. Chem. 256, 6593-6599.
2. 2. Smyth, G. E., and Colman, R. F. (1991) Cysteinyl peptides of pig heart NADP-dependent isocitrate dehydrogenase which are modified upon inactivation by N-ethylmaleimide. J. Biol. Chem. 266, 14918-14925.
3. 2+-isocitrate. J. Biol. Chem. 268, 5264-5271.
4. 4. Haselbach, R. J., Colman, R. F., and McAlister-Henn, L. (1992) Isolation and sequence of a cDNA encoding porcine mitochondrial NADP-specific isocitrate dehydrogenase. Biochemistry 31, 6219-6223.
5. 5. Kelly, J. H., and Plaut, G. W. E. (1981) Physical evidence for the dimerization of the TPN-specific isocitrate dehydrogenase from pig heart. J. Biol. Chem. 256, 330-334.
6. 6-ethenoadenosine 2′,5′-diphosphate. Biochemistry 24, 5367-5377.
7. 7. Colman, R. F. (1983) Aspects of the structure and function of the isocitrate dehydrogenases. Peptide Protein Rev. 1, 41-69.
8. 8. Colman, R. F. (1989) Affinity labels and spectroscopic probes of porcine heart NADP-dependent isocitrate dehydrogenase. Biochem. Soc. Trans. 17, 307-311.
9. --specific isocitrate dehydrogenase. Biochemistry 23, 1675-1683.
10. + analogues to NAD and NADP-dependent pig heart isocitrate dehydrogenases. A nuclear magnetic resonance study. Biochemistry 31, 12524-12531.
11. 11. Ehrlich, R. S., and Colman, R. F. (1989) Multinuclear NMR studies of the divalent metal binding site of NADP-dependent isocitrate dehydrogenase from pig heart. Biochemistry 28, 2058-2065.
12. 25Mg NMR study of the divalent metal binding sites of isocitrate dehydrogenases from pig heart. Biochim. Biophys. Acta 1246, 135-141.
13. 13C NMR of substrate binding. Biochemistry 26, 3461-346.
14. 14. Hurley, J. H., Thorsness, P. E., Ramalingam, V., Helmers, N. H., Koshland, D. E., and Stroud, R. M. (1989) Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase. Proc. Natl. Acad. Sci. USA 86, 8635-8639.
15. 15. Hurley, J. H., Dean, A. M., Koshland, D. E., and Stroud, R. M. (1991) Catalytic mechanism of NADP-dependent isocitrate dehydrogenase: Implications from the structures of magnesium-isocitrate and NADP complexes. Biochemistry 30, 8671-8678.
16. 16. Stoddard, B. L., Dean, A., and Koshland, D. E., Jr. (1993) Structure of isocitrate dehydrogenase with isocitrate, NADP and calcium at 2.5 Å resolution: A pseudo-Michaelis ternary complex. Biochemistry 32, 9310-9316.
17. 17. Nagai, K., and Thogersen, H. C. (1987) Synthesis and sequence-specific proteolysis of hybrid proteins produced in E. coli. Methods Enzymol. 153, 461-481.
18. 18. Takagi, T., and Doolittle, R. F. (1974) Amino acid sequence studies on factor XIII and the peptide released during its activation by thrombin. Biochemistry 13, 750-756.
19. 19. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) in "Molecular Cloning: A Laboratory Manual," 2nd ed. (Ford, N., Ed.), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
20. 20. Zoller, M. J., and Smith, M. (1982) Oligonucleotide-directed mutagenesis using M13-derived vectors: An efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res. 10, 6487-6506.
21. 21. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
22. 22. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254.
23. 23. Johanson, R. A., and Colman, R. F. (1981) Arch. Biochem. Biophys. 207, 9-20.
24. 24. Kellerman, O. K., and Ferenci, T. (1982). Maltose binding protein from E. coli. Methods in Enzymol. 90, 459-463.
25. 25. Blackshear, P. J. (1984) Methods in Enzymol. 104, 237-255.
26. 26. Suzuki, M., Sahara, T., Tsuruha, J.-I., Takada, Y., and Fakunaga, N. (1995) Differential expression in Escherichia coli of the Vibrio sp. strain ABE-1 icdI and icdII genes encoding structurally different isocitrate dehydrogenase isozymes. J. Bacteriol. 177, 2138-2142.
27. +-dependent isocitrate dehydrogenase using resonance energy transfer. Biochemistry 26, 4893-4900.