Fa1p is a 171 kDa protein essential for axonemal microtubule severing in Chlamydomonas

Journal of Cell Science

Volumn 113, Issue 11, 2000, Pages 1963-1971

  Finst, Rip J ^a   Kim, Peter J ^a   Griffis, Eric R ^a   Quarmby, Lynne M ^a,b  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b SIMON FRASER UNIVERSITY   (Canada)

Author keywords

Axoneme; Chlamydomonas; Flagellum; Microtubule severing

Indexed keywords

ALGAL PROTEIN; CALCIUM BINDING PROTEIN; COMPLEMENTARY DNA; DNA;

ALLELE; AMINO TERMINAL SEQUENCE; ARTICLE; AXONEME; CELL FRACTIONATION; CENTROMERE; CHLAMYDOMONAS; FLAGELLUM; GENE MAPPING; MATING TYPE; MICROTUBULE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PSEUDOPODIUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BLOTTING, NORTHERN; CALCIUM; CALCIUM SIGNALING; CALMODULIN; CHLAMYDOMONAS REINHARDTII; CHROMOSOME MAPPING; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FLAGELLA; MICROTUBULE PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS; PROTEIN STRUCTURE, SECONDARY; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA;

ALGAE; CHLAMYDOMONAS; INVERTEBRATA;

EID: 0034049946     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. and Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25, 3389-3402.
2. Anderson, R. G. W. (1974). Isolation of ciliated or unciliated basal bodies from the rabbit oviduct. J. Cell Biol. 60, 393-404.
3. Auclair, W. and Siegel, B. W. (1966). Cilia regeneration in the sea urchin embryo: evidence for a pool of ciliary proteins. Science 154, 913-915.
4. Beck, K. and Brodsky, B. (1998). Supercoiled protein motifs: the collagen triple-helix and alpha-helical coiled-coil. J. Struct. Biol. 122, 17-29.
5. Debuchy, R., Purton, S. and Rochaix, J. D. (1989). The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J. 8, 2803-2809.
6. Dutcher, S. K. (1995). Purification of basal bodies and basal body complexes from Chlamydomonas reinhardtii. In Cilia and Flagella (ed. W. Dentier and G. Witman), pp. 323-334. San Diego, CA: Academic Press.
7. Eyal, Y., Neumann, H., Or, E. and Frydman, A. (1999). Inverse single-strand RACE: An adaptor-independent method of 5′ RACE. BioTechniques 27, 656-658.
8. Fernandez, E., Schnell, R., Ranum, L. P. W., Hussey, S. C., Silflow, C. D. and Lefebvre P. A. (1989). Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. Proc. Nat. Acad. Sci. USA 86, 6449-6453.
9. Ferris, P. J. and Goodenough, U. W. (1994). The mating-type locus of Chlamydomonas reinhardtii contains highly rearranged DNA sequences. Cell 76, 1135-1145.
10. Finst, R. J., Kim, P. J. and Quarmby, L. M. (1998). Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149, 927-936.
11. Gitelman, S. E. and Witman, G. B. (1980). Purification of calmodulin from Chlamydomonas: calmodulin occurs in cell bodies and flagella. J. Cell Biol. 87, 764-70.
12. Harris, E. 'H. (1989). The Chlamydomonas Sourcebook. Berkeley, CA: Academic Press.
13. Hartman, J. J., Mahr, J., McNally, K., Okawa, K., Inamatsu, A., Thomas, S., Cheesman, S., Heuser, J., Vale, R. D. and McNally, F. J. (1998). Katanin, a microtubule-severing protein, is a novel AAA ATPase that target to the centrosome using a WD40-containing subunit. Cell 93, 277-287.
14. Hastie, A. T., Dicker, D. T., Hingley, S. T., Kueppers, F., Higgins, M. L. and Weinbaum, G. (1986). Isolation of cilia from porcine tracheal epithelium and extraction of dynein arms. Cell Motil. Cytoskel. 6, 25-34.
15. Jarvik, J. W. and Suhan, J. P. (1991). The role of the flagellar transition region: Inferences from the analysis of a Chlamydomonas mutant with defective transition region structures. J. Cell Sci. 99, 731-740.
16. Heimburg, T., Schunemann, J., Weber, K. and Geisler, N. (1999). FTIR-spectroscopy of multistranded coiled coil proteins. Biochemistry 38, 12727-12734.