Inverse single-strand RACE: An adapter-independent method of 5' RACE

BioTechniques

Volumn 27, Issue 4, 1999, Pages 656-658

  Eyal, Yoram ^a   Neumann, Hanna ^a   Or, Etti ^a   Frydman, Ahuva ^a  

^a VOLCANI CENTER   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

SINGLE STRANDED DNA;

ARTICLE; DNA PURIFICATION; DNA SEQUENCE; GENE AMPLIFICATION; GENE ISOLATION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION;

EID: 0032883671     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99274bm04     Document Type: Article

References (8)

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3. Kunik, T., L. Mizrachy, V. Citovsky and Y. Gafni. 1999. Characterization of a tomato karyopherin-α that interacts with the Tomato Yellow Leaf Curl Virus (TYLCV) capsid protein. J. Exp. Botany 50:731-732.
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5. Schaefer, B.C. 1995. Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends. Anal. Biochem. 227:255-273.
6. ShengHe, H., H. Yihuan, W. Chunhua and J. Holcenberg. 1990. A simple method for direct cloning cDNA sequence that flanks a region of known sequence from total RNA by applying the inverse polymerase chain reaction. Nucleic Acids Res. 18:1922.
7. Sinclair, M.C. and R. Aitken. 1995. PCR strategies for isolation of the 5′ end of an immunoglobulin-encoding bovine cDNA. Gene 167:285-289.
8. Troutt, A.B., M.G. McHeyzer-Williams, B. Pulendran and G.J. Nossal. 1992. Ligation-anchored PCR: a simple amplification technique with single-sided specificity. Proc. Natl. Acad. Sci. USA 89:9823-9825.