Improved FACS analysis confirms generation of immature dendritic cells in long-term stromal-dependent spleen cultures

Immunology and Cell Biology

Volumn 78, Issue 3, 2000, Pages 196-204

  Ni, Keping ^a   O'Neill, Helen C ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

Autofluorescence; Dendritic cell; Fluorescence activated cell sorter analysis; Long term culture; Quenching

Indexed keywords

ANIMAL CELL; ARTICLE; AUTOFLUORESCENCE; CELL CULTURE; CELL PROLIFERATION; DENDRITIC CELL; FLUORESCENCE ACTIVATED CELL SORTER; IMMUNOASSAY; MOUSE; NONHUMAN; SPLEEN CELL;

ANIMALS; ANTIGENS, CD; ANTIGENS, CD11; ANTIGENS, CD80; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELL SEPARATION; DENDRITIC CELLS; FLOW CYTOMETRY; GENTIAN VIOLET; HLA ANTIGENS; HUMANS; LECTINS, C-TYPE; MEMBRANE GLYCOPROTEINS; MICE; RECEPTORS, CELL SURFACE; RECEPTORS, FC; SPLEEN; STROMAL CELLS;

ANIMALIA; VIOLA (ANGIOSPERM);

EID: 0034049746     PISSN: 08189641     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1711.2000.00897.x     Document Type: Article

References (26)

1. 1 Ni K, O'Neill HC. Long-term stromal cultures produce dentritic-like cells. Br. J. Haematol. 1997; 97: 710-25.
2. 2 Ni K, O'Neill HC. Hemopoiesis in long-term stroma-dependent cultures from lymphoid tissues: Production of cells with myeloid/dendritic characteristics. In Vitro Cell. Dev. Biol. Anim. 1998; 34: 298-307.
3. 3 Ni K, O'Neill HC. Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow. Br. J. Haematol. 1999; 105: 58-67.
4. 4 Sharrow SO. Analysis of flow cytometry data. In: Coliau JE, Kruisbeek AM, Marjulies DH (eds). Current Protocols in Immunology. New York: Wiley Interscience, 1994; section 5.2.1.
5. 5 O'Neill HC, Ni K. Characterization of unique lymphoid cells derived from murine spleen which constitutively produce interleukin-6. Immunology 1993; 79: 220-9.
6. 6 Skold CM, Eklund A, Hallden G, Hed J. Autofluorescence in human alveolar macrophages from smokers: Relation to cell surface markers and phagocytosis. Exp. Lung Res. 1989; 15: 823-35.
7. 7 Hallden G, Andersson U, Hed J, Johnsson SG. A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry. J. Immunol. Methods 1989; 124: 103-9.
8. 8 Hallden G, Skold CM, Eklund A, Forslid J, Hed J. Quenching of intracellular autofluorescence in alveolar macrophages permits analysis of fluorochrome labelled surface antigens by flow cytofluorometry. J. Immunol. Methods 1991; 142: 207-14.
9. 9 Ruedl C, Hubele S. Maturation of Peyer's patch dendritic cells in vitro upon stimulation via cytokines or CD40 triggering. Eur. J. Immunol. 1997; 27: 1325-30.
10. 10 Stosel H, Koch F, Romani N. Maturation and migration of murine dendritic cells in situ. Observations in a skin organ culture model. Adv. Exp. Med. Biol. 1997; 17: 311-15.
11. 11 Inaba K, Steinman RM, Witmer-Pack M et al. Identification of proliferating dendritic cell precursors in mouse blood. J. Exp. Med. 1992; 175: 1157-67.
12. 12 Inaba K, Inaba M, Deguchi M et al. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 1992; 176: 1693-702.
13. 13 Lu L, Woo J, Rao AS et al. Propagation of dendritic cell progenitors from normal mouse liver using granulocyte/macrophage colony-stimulating factor and their maturational development in the presence of type-1 collagen. J. Exp. Med. 1994; 179: 1823-34.
14. 14 Olweus J, BitMansour A, Warnke R et al. Dendritic cell ontogeny: A human dendritic cell lineage of myeloid origin. Proc. Natl Acad. Sci. USA 1997; 94: 12551-6.
15. 15 Wu L, Li CL, Shortman K. Thymic dendritic cell precursors: Relationship to the T lymphocyte lineage and phenotype of the dendritic cell progeny. J. Exp. Med. 1996, 184: 903-11.
16. 16 Vremec D, Shortman K. Dendritic cell subtypes in mouse lymphoid organs: Cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes. J. Immunol. 1997; 159: 565-73.
17. 17 Hed J. The extinction of fluorescence by crystal violet and its use to differentiate between attached and ingested microorganisms in phagocytosis. FEMS Lett. 1977; 1: 357-60.
18. 18 Ma JA, Chapman GV, Chen SL, Penny R, Breit SN. Flow cytometry with crystal violet to detect intracytoplasmic fluorescence in viable human lymphocytes. Demonstration of antibody entering living cells. J. Immunol. Methods 1987; 104: 195-200.
19. 19 Skold CM, Lundahl J, Hallden M, Hallgren M, Eklund A. Chronic smoker exposure alters the phenotype pattern and the metabolic response in human alveolar macrophages. Clin. Exp. Immunol. 1996; 106: 108-13.
20. 20 Young JW, Szabolcs P, Moore MA. Identification of dendritic cell colony-forming units among normal human CD34+ bone marrow progenitors that are expanded by c-kit-ligand and yield pure dendritic cell colonies in the presence of granulocyte/macrophage colony-stimulating factor and tumor necrosis factor alpha. J. Exp. Med. 1995; 182: 1111-19.
21. 21 Gieseler R, Heise D, Soruri A, Schwartz P, Peters HJ. In vitro differentiation of mature dendritic cells from human blood monocytes. Dev. Immunol. 1998; 6: 25-39.
22. 22 Maraskovsky E, Brasel K, Teepe M et al. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: Multiple dendritic cell subpopulations identified. J. Exp. Med. 1996; 184: 1953-62.
23. 23 Schuler G, Steinman RM. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med. 1985; 161: 526-46.
24. 24 Leenen PJ, Radosevic K, Voerman JSA et al. Heterogeneity of mouse spleen dendritic cells: In vitro phagocytic activity, expression of macrophage markers, and subpopulation turnover. J. Immunol. 1998; 160: 2166-73.
25. 25 Saunders D, Lucas K, Ismaili J et al. Dendritic cell development in culture from thymic precursor cells in the absence of granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 1996; 184: 2185-96.
26. 26 Vremec D, Lieschke GJ, Dunn AR, Robb L, Metcalf D, Shortman K. The influence of granulocyte/macrophage colony-stimulating factor on dendritic cell levels in mouse lymphoid organs. Eur. J. Immunol. 1997; 27: 40-4.