Expression of active monomeric and dimeric nuclease A from the gram-positive Streptococcus gordonii surface protein expression system

Protein Expression and Purification

Volumn 19, Issue 1, 2000, Pages 158-172

  Dutton, Emma K ^a   Ottum, Sean A ^a   Bolken, Tove C ^a   Franke, Christine A ^a,b   Hruby, Dennis E ^a,b  

^a SIGA Technologies Incorporated   (United States)

^b OREGON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SECRETION; CELL SURFACE; DRUG DELIVERY SYSTEM; ENZYME SYNTHESIS; GENE DELETION; GENE EXPRESSION SYSTEM; HOMOLOGOUS RECOMBINATION; NUCLEASE A; NUCLEOTIDE SEQUENCE; PH; RECOMBINANT ENZYME; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS GORDONII;

EID: 0034048239     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1223     Document Type: Article

References (42)

1. Evens R. P., Witcher M. Biotechnology: An introduction to recombinant DNA technology and product availability. Ther. Drug Monit. 15:1993;514-520.
2. Johnson I. S. Human insulin from recombinant DNA technology. Science. 219:1983;632-637.
3. Ada G. Overview of vaccines. Mol. Biotechnol. 8:1997;123-134.
4. Geisow M. J. Both bane and blessing - Inclusion bodies. Trends Biotechnol. 9:1991;368-369.
5. Rosenfeld S. A. Use of Pichia pastoris for expression of recombinant proteins. Methods Enzymol. 306:1999;154-169.
6. Myscofski D. M., Hruby D. E. SPEX, a system for the expression of recombinant proteins from gram-positive bacterial vectors. Protein Express. Purif. 14:1998;409-4517.
7. Pakula R., Fluder Z., Hulanicka E., Walczak W. Studies on transformation of streptococci. Bull. Acad. Pol. Sci. Ser. Sci. Biol. 6:1958;319-323.
8. Lindberg A. A. The history of live bacterial vaccines. Dev. Biol. Stand. 84:1995;211-219.
9. Navarre W. W., Schneewind O. Surface proteins of Gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Micro. Mol. Biol. Rev. 63:1999;174-229.
10. Pozzi G., Contorni M., Oggioni M. R., Manganelli R., Tommasino M., Cavalieri F., Fischetti V. A. Delivery and expression of a heterologous antigen on the surface of streptococci. Infect. Immun. 60:1992;1902-1907.
11. Pozzi G., Oggioni M. R., Maganelli R., Fischetti V. A. Expression of M6 protein gene of Streptococcus pyogenes in Streptococcus gordonii after chromosomal integration and transcriptional fusion. Res. Microbiol. 143:1992;449-457.
12. Pozzi G., Oggioni M. R., Manganelli R., Medaglini D., Fischetti V. A., Fenoglio D., Valle M. T., Kunkl A., Manca F. Human T-helper cell recognition of an immunodominant epitope of HIV-1 gp120 expressed on the surface of Streptococcus gordonii. Vaccine. 12:1994;1071-1077.
13. Oggioni M. R., Medaglini D., Romano L., Peruzzi F., Maggi T., Lozzi L., Bracci L., Zazzi M., Manca F., Valensin P. E., Pozzi G. Antigenicity and immunogenicity of the V3 domain of HIV type 1 glycoprotein 120 expressed on the surface of Streptococcus gordonii. AIDS Res. Hum. Retroviruses. 15:1999;451-459.
14. Di Fabio S., Medaglini D., Rush C. M., Corrias F., Panzini G. L., Pace M., Verani P., Pozzi G., Titti F. Vaginal immunization of Cynomolgus monkeys with Streptococcus gordonii expressing HIV-1 and HPV 16 antigens. Vaccine. 16:1998;485-492.
15. Lee S. F., March R. J., Halperin S. A., Faulkner G., Gao L. Surface expression of a protective recombinant pertussis toxin S1 subunit fragment in Streptococcus gordonii. Infect. Immun. 67:1999;1511-1516.
16. Motin V. L., Jones K. F., de la Maza L. M., Fischetti V. A., Peterson E. M. Display of the variable domain 4 (VD4) of Chlamydia trachomatis MOMP on the surface of Streptococcus gordonii. Stephens R. S., Byrne G. I., Christensen G., Clarke I. N., Grayston J. T., Rank R. G., Ridgway G. L., Sakaguchi M., Schachter J., Stamm W. E. Chlamydia Infections: Proceedings of the 9th International Symposium on Human Chlamydial Infections - International Chlamydia Symposium. 1998;547-550.
17. Sharma A., Nagata H., Hamada N., Sojar H. T., Hruby D. E., Kuramitsu H. K., Genco R. J. Expression of functional Porphyromonas gingivalis fimbrillin polypeptide domains on the surface of Streptococcus gordonii. Appl. Environ. Microbiol. 62:1996;3933-3938.
18. Sharma A., Sojar H. T., Hruby D. E., Kuramitsu H. K., Genco R. J. Secretion of Porphyromonas gingivalis fimbrillin polypeptides by recombinant Streptococcus gordonii. Biochem. Biophys. Res. Commun. 238:1997;313-316.
19. Sharma A., Honma K., Sojar H. T., Hruby D. E., Kuramitsu H. K., Genco R. J. Expression of saliva-binding epitopes of the Porphyromonas gingivalis FimA protein on the surface of Streptococcus gordonii. Biochem. Biophys. Res. Commun. 258:1999;222-226.
20. Coyle T. C., Franke C. A., Hruby D. E. Development of a radioactive protein A-based assay for analysis of surface protein expression in gram-positive bacteria. Appl. Environ. Micro. 63:1997;2477-2480.
21. Medaglini D., Pozzi G., King T. P., Fischetti V. A. Mucosal and systemic immune responses to a recombinant protein expressed on the surface of the oral commensal bacterium Streptococcus gordonii after oral colonization. Proc. Natl. Acad. Sci. USA. 92:1995;6868-6872.
22. Rescigno M., Citterio S., Thery C., Rittig M., Medaglini D., Pozzi G., Amigorena S., Ricciardi-Castagnoli P. Bacteria-induced neo-biosynthesis, stabilization, and surface expression of functional class I molecules in mouse dendritic cells. Proc. Natl. Acad. Sci. USA. 95:1998;5229-5234.
23. Poquet I., Dusko Ehrlich S., Gruss A. An export-specific reporter designed for gram-positive bacteria: Application to Lactococcus lactis. J. Bacteriol. 180:1998;1904-1912.
24. Lieble W., Sinskey A. J., Schleifer K.-H. Expression, secretion, and processing of staphylococcal nuclease by Corynebacterium glutamicum. J. Bacteriol. 174:1992;1854-1861.
25. Kovaceivic S., Veal L. E., Hsiung H. M., Miller J. R. Secretion of staphylococcal nuclease by Bacillus subtilis. J. Bacteriol. 162:1985;521-528.
26. Green S. M., Gittis A. G., Meeker A. K., Lattman E. E. One-step evolution of a dimer from a monomeric protein. Nature Struct. Biol. 2:1995;746-751.
27. Fischetti V. A., Medaglini D., Oggioni M., Pozzi G. Expression of foreign proteins on Gram-positive commensal bacteria for mucosal vaccine delivery. Curr. Opin. Biotechnol. 4:1993;603-610.
28. Oggioni M. R., Pozzi G. A host-vector system for heterologous gene expression in Streptococcus gordonii. Gene. 169:1996;85-90.
29. Oggioni M. R., Medaglini D., Maggi T., Pozzi G. Engineering the Gram-positive cell surface for construction of bacterial vaccine vectors. Methods. 19:1999;163-173.
30. Lachica R. V. F., Genigeorgis C., Hoeprich P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl. Microbiol. 21:1971;585-587.
31. Van de Rijn I., Kessler R. E. Growth characteristics of group A streptococci in a new chemically defined medium. Infect. Immun. 27:1980;444-448.
32. Cuatrecasas P., Fuchs S., Anfinsen C. B. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus. J. Biol. Chem. 242:1967;1541-1547.
33. Heins J. N., Suriano J. R., Taniuchi H., Anfinsen C. B. Characterization of a nuclease produced by Staphylococcus aureus. J. Biol. Chem. 242:1967;1016-1020.
34. Cuatrecasas P., Fuchs S., Anfinsen C. B. The binding of nucleotides and calcium to the extracellular nuclease of Staphylococcus aureus. J. Biol. Chem. 242:1967;3063-3067.
35. Penzikova G. A., Oreshina M. G., Levitov M. M. Nuclease activity in the cultural fluid filtrate of actinomycetes - Products of antibiotics. Prikl Biokhim. Mikrobiol. 10:1974;222-226.
36. Okazaki R., Okazaki T., Sakabe K. An extracellular nuclease of Bacillus subtilis: Some novel properties as a DNA exonuclease. Biochem. Biophys. Res. Commun. 22:1966;611-619.
37. Kapranova M. N., Balaban N. P., Golubenko I. A., Leshchinskaia I. B. Extracellular nuclease of Bacillus mesentericus. Biokhimiia. 41:1976;639-642.
38. Miller A., Sandine W. E., Elliker P. R. Extracellular nuclease in the genus Lactobacillus. J. Bacteriol. 108:1971;604-606.
39. Seto H., Lopez R., Garrigan O., Tomasz A. Nucleolytic degradation of homologous and heterologous deoxyribonucleic acid molecules at the surface of competent pneumococci. J. Bacteriol. 122:1975;676-685.
40. Ferreira B. T., Benchetrit L. C., De Castro A. C., Batista T. G., Barrucand L. Extracellular deoxyribonucleases of streptococci: A comparison of their occurrence and levels of production among beta-hemolytic strains of various serological groups. Zentralbl. Bakteriol. 277:1992;493-503.
41. Taniuchi H., Afinsen C. B., Sodja A. The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. III. Complete amino acid sequence. J. Biol. Chem. 242:1967;4752-4758.
42. Udou T., Ichik'awa Y. Effect of sodium chloride on the activity and production of staphylococcal exonuclease. J. Gen. Microbiol. 116:1980;69-74.