Detection of clonal T cell populations by high resolution PCR using fluorescently labelled nucleotides; evaluation using conventional LIS-SSCP

Journal of Clinical Pathology - Molecular Pathology

Volumn 53, Issue 3, 2000, Pages 150-154

  Wickham, C L ^a   Lynas, C ^c   Ellard, S ^b  

^a ROYAL DEVON AND EXETER NHS FOUNDATION TRUST   (United Kingdom)

^b UNIVERSITY OF EXETER   (United Kingdom)

^c DERRIFORD HOSPITAL   (United Kingdom)

Author keywords

DNA sequencer; Low ionic strength single strand conformation polymorphism (SSCP); T cell clonality; T cell receptor gene rearrangement

Indexed keywords

T LYMPHOCYTE RECEPTOR;

ARTICLE; CANCER GENETICS; CLINICAL ARTICLE; COMPUTER PROGRAM; DNA DETERMINATION; GENE AMPLIFICATION; GENE REARRANGEMENT; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; T CELL LEUKEMIA; T CELL LYMPHOMA; T LYMPHOCYTE SUBPOPULATION;

CLONE CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EVALUATION STUDIES; HUMANS; LEUKEMIA, T-CELL; LYMPHOMA, T-CELL; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA; T-LYMPHOCYTE SUBSETS;

EID: 0034045618     PISSN: 13668714     EISSN: None     Source Type: Journal    
DOI: 10.1136/mp.53.3.150     Document Type: Article

References (14)

1. 1 McCarthy KP, Sloane JP, Kabarowski JHS, et al. A simplified method of detection of clonal rearrangements of T cell receptor γ chain gene. Diagn Mol Pathol 1992;1:173 9.
2. 2 Slack DN, McCarthy KP, Wiedemann LM, et al. Evaluation of sensitivity, specificity, and reproducibility of an optimized method for detecting clonal rearrangements of immunoglobulin and T-cell receptor genes in formalinfixed, paraffin-embedded sections. Diagn Mol Pathol 1993; 2:223-32.
3. 3 Ashton-Key M, Diss TC, Du MQ, et al. The value of the polymerase chain reaction in the diagnosis of cutaneous T-cell infiltrates. Am J Surg Pathol 1997;21:743-7.
4. 4 Lynas C, Howe D, Copplestone JA, et al. A rapid and reliable PCR method for detecting clonal T cell populations. J Clin Pathol: Mol Pathol 1995;48:M101-4.
5. 5 Lynas C, Howe D. Additional TCRVB primers and minor method modifications improve detection of clonal T cell populations by RT-PCR. J Clin Pathol 1997;50:53-5.
6. 6 Theodorou I, Delfau-Larue MH, Bigorgne C, et al. Cutaneous T-cell infiltrates: analysis of T-cell receptor γ gene rearrangement by polymerase chain reaction and denaturing gradient gel electrophoresis. Blood 1995;86:305-10.
7. 7 Lynas C, Howe D. Simple and reliable detection of T cell clones by PCR-LIS-SSCP analysis of TCRγ rearrangement. Mol Cell Probes 1998;12:41-8.
8. 8 Kneba M, Bolz I, Linke B, et al. Characterisation of clone-specific rearranged T-cell receptor γ-chain genes in lymphomas and leukemias by polymerase chain reaction and DNA sequencing. Blood 1994;84:574-81.
9. 9 Orita M, Iwnhana H, Kanazawa H, et al. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sci U S A 1989;86:2766-70.
10. 10 Maruya E, Saji H, Yokoyama S. PCR-LIS-SSCP (low ionic strength single-stranded conformation polymorphism) - a simple method for high-resolution allele typing of HLA-DRB1, DQB1 and DPB1. Genome Res 1996;6:51-7.
11. 11 Witzens M, Mohler T, Willhauk M, et al. Detection of clonally rearranged T-cell receptor gamma chain genes from T-cell malignancies and acute inflammatory rheumatic disease using PCR amplification, PAGE, and automated analysis. Ann Hematol 1997;74:123-30.
12. 12 Simon M, Kind P, Kaudewitz P, et al. Automated high-resolution polymerase chain reaction fragment analysis - a method for detecting T-cell receptor gamma-chain gene rearrangements in lymphoproliferative diseases. Am J Pathol 1998;152:29-33.
13. 13 Kneba M, Bolz I, Linke B, et al. Analysis of rearranged T-cell receptor B-chain genes by polymerase chain reaction (PCR), DNA sequencing and automated high resolution PCR fragment analysis. Blood 1995;86:3930-7.
14. 14 Shiach CR, Evans PA, Short MA, et al. Detection and accurate sizing of PCR products by automated scanning: improved detection of immunoglobulin gene rearrangement in ALL. Br J Haematol 1993;85:431-2.