PCR-LIS-SSCP (low ionic strength single-stranded conformation polymorphism) - A simple method for high-resolution allele typing of HLA-DRB1, -DQB1, and -DPB1

Genome Research

Volumn 6, Issue 1, 1996, Pages 51-57

  Maruya, Etsuko ^a   Saji, Hiroh ^a   Yokoyama, Sigeki ^a  

^a JAPANESE RED CROSS SOCIETY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; HLA DP ANTIGEN; HLA DQ ANTIGEN; HLA DR ANTIGEN; SINGLE STRANDED DNA;

ALLELE; ARTICLE; CONFORMATIONAL TRANSITION; DNA DENATURATION; DNA DETERMINATION; DNA POLYMORPHISM; ELECTROPHORETIC MOBILITY; GENE AMPLIFICATION; HLA TYPING; HUMAN; HUMAN CELL; IONIC STRENGTH; MAJOR CLINICAL STUDY; NORMAL HUMAN; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM;

ALLELES; DNA, SINGLE-STRANDED; HISTOCOMPATIBILITY TESTING; HLA-DP ANTIGENS; HLA-DQ ANTIGENS; HLA-DR ANTIGENS; HUMANS; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL;

EID: 0029871081     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.6.1.51     Document Type: Article

References (10)

1. Babbit, B.P., P.M. Allen, G. Matsueda, E. Haber, and E.R. Unanue. 1985. Binding of immunogenic peptides to la histocompatibility molecules. Nature 317: 359-361.
2. Bach, F.H. 1985. Class II genes and products: HLA-D region. Immunol. Today 6: 89-94.
3. Bach, F. and D.H. Sachs. 1987. Transplanted immunology. N. Engl. J. Med. 317: 489-492.
4. Bannai, M., K. Tokunaga, L. Lin, S. Kuwata, T. Mazda, I. Amaki, K. Fujikawa, and T. Juji. 1994. Discrimination of human HLA-DRB1 alleles by PCR-SSCP (single-strand conformation polymorphism) method. Bur. J. Immunogenet. 21: 1-9.
5. Blasczyk, R., U. Hahn, J. Wehling, D. Huhn, and A. Salama. 1995. Complete subtyping of the HLA-A locus by sequence-specific amplification followed by direct sequencing or single-strand conformation polymorphism analysis. Tissue Antigens 46: 86-95.
6. Bodmer, J.G., S.G.E. Marsh, E.D. Albert, W.F. Bodmer, R.E. Bontrop, D. Charron, B. Dupont, H.A. Erlich, B. Mach, W.R. Mayr, P. Parham, T. Sasazuki, G.M.T. Schreuder, J.L. Strominger, A. Svejgaard, and P.I. Terasaki. 1995. Nomenclature for factors of the HLA system, 1995. Hum. Immunol. 43: 149-164.
7. Buus, S., A. Sette, S. Colon, C. Miles, and H.M. Grey. 1987. The relation between major histocompatibility complex (MHC) restriction and the capacity of la to bind immunogenic peptides. Science 235: 1353-1358.
8. Guillet, J.G., M.Z. Lai, and T.J. Briner. 1987. Immunological self/nonself discrimination. Science 235: 865-870.
9. Hoshino, S., A. Kimura, Y. Fukuda, K. Dohi, and T. Sasazuki. 1992. PCR-SSCP analysis of polymorphism in DPA1 and DPB1 genes: Simple and rapid method for histocompatibility test. Hum. Immunol. 33: 98-108.
10. Kawai, S., S. Maekawajiri, K. Tokunaga, T. Juji, and A. Yamane. 1994. A simple method of HLA-DRB typing using enzymatically amplified DNA and immobilized probes on microtiter plates. Hum. Immunol. 41: 121-126.