The Fusobacterium nucleatum porin FomA possesses the general topology of the non-specific porins

Microbiology

Volumn 146, Issue 6, 2000, Pages 1437-1445

  Puntervoll, Pål ^a   Kleivdal, Hans ^a   Dahl, Karl Ole ^a   Bitter, Wilbert ^b   Tommassen, Jan ^b   Jensen, Harald B ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Epitope insertion mutagenesis; Fusobacterium nucleatum; Outer membrane proteins; Topology

Indexed keywords

BACTERIAL PROTEIN; PORIN;

ARTICLE; FUSOBACTERIUM NUCLEATUM; GENE INSERTION; IMMUNOFLUORESCENCE MICROSCOPY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; SEMLIKI FOREST ALPHAVIRUS;

ALPHAVIRUS; BACTERIA (MICROORGANISMS); FUSOBACTERIUM NUCLEATUM; NEGIBACTERIA; RABBIT FIBROMA VIRUS; SEMLIKI FOREST VIRUS;

EID: 0034043955     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-6-1437     Document Type: Article

References (39)

1. Agterberg, M., Adriaanse, H. & Tommassen, J. (1987). Use of outer membrane protein PhoE as a carrier for the transport of a foreign antigenic determinant to the cell surface of Escherichia coli K-12. Gene 59, 145-150.
2. Bakken, V., Aaro, S., Hofstad, T. & Vasstrand, E. N. (1989a). Outer membrane proteins as major antigens of Fusobacterium nucleatum. FEMS Microbiol Immunol 1, 473-483.
3. Bakken, V., Aaro, S. & Jensen, H. B. (1989b). Purification and partial characterization of a major outer-membrane protein of Fusobacterium nucleatum. J Gen Microbiol 135, 3253-3262.
4. Bolstad, A. I., Tommassen, J. & Jensen, H. B. (1994). Sequence variability of the 40-kDa outer membrane proteins of Fusobacterium nucleatum strains and a model for the topology of the proteins. Mol Gen Genet 244, 104-110.
5. Bolstad, A. I., Hogh, B. T. & Jensen, H. B. (1995). Molecular characterization of a 40-kDa outer membrane protein, FomA, of Fusobacterium periodonticum and comparison with Fusobacterium nucleatum. Oral Microbiol Immunol 10, 257-264.
6. Bolstad, A. I., Jensen, H. B. & Bakken, V. (1996). Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev 9, 55-71.
7. Charbit, A., Boulain, J. C., Ryter, A. & Hofnung, M. (1986). Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope; expression at the cell surface. EMBO J 5, 3029-3037.
8. Cowan, S. W. (1993). Bacterial porins: lessons from three high-resolution structures. Curr Opin Struct Biol 3, 501-507.
9. Cowan, S. W., Schirmer, T., Rummel, G., Steiert, M., Ghosh, R., Pauptlt, R. A., Jansonius, J. N. & Rosenbusch, J. P. (1992). Crystal structures explain functional properties of two E. coli porins. Nature 358, 727-733.
10. Dekker, N., Merck, K., Tommassen, J. & Verheij, H. M. (1995). In vitro folding of Escherichia coli outer-membrane phospholipase A. Eur J Biochem 232, 214-219.
11. Diederichs, K., Freigang, J., Umhau, S., Zeth, K. & Breed, J. (1998). Prediction by a neural network of outer membrane β-strand protein topology. Protein Sci 7, 2413-2420.
12. Fernandez, I. M., Harmsen, M., Benaissa-Trouw, B. J., Stuij, I., Puyk, W., Meloen, R. H., Snippe, H. & Kraaijeveld, C. A. (1998). Epitope polarity and adjuvants influence the fine specificity of the humoral response against Scmliki Forest virus specific peptide vaccines. Vaccine 16, 1531-1536.
13. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580.
14. Heller, K. B. (1978). Apparent molecular weights of a heat-modifiable protein from the outer membrane of Escherichia coli in gels with different acrylamide concentrations. J Bacteriol 134, 1181-1183.
15. Ho, S. N., Hunt H. D., Horton, R. M., Pullen, J. K. & Pease, L. R. (1989). Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59.
16. Jensen, H. B., Skeidsvoll, J., Fjellbirkeland, A., Hogh, B., Puntervoll, P., Kleivdal, H. & Tommassen, J. (1996). Cloning of the fomA gene, encoding the major outer membrane porin of Fusobacterium nucleatum ATCC10953. Microb Pathog 21, 331-342.
17. Kaufman, J. & DiRienzo, J. M. (1989). Isolation of a corncob (coaggregation) receptor polypeptide from Fusobacterium nucleatum. Infect Immun 57, 331-337.
18. Kleivdal, H., Benz, R. & Jensen, H. B. (1995). The Fusobacterium nucleatum major outer-membrane protein (FomA) forms trimeric, water-filled channels in lipid bilayer membranes. Eur J Biochem 233, 310-316.
19. Kleivdal, H., Benz, R., Tommassen, J. & Jensen, H. B. (1999). Identification of positively charged residues of FomA porin of Fusobacterium nucleatum which are important for pore function. Eur J Biochem 260, 818-824.
20. Kolenbrander, P. E. & London, J. (1993). Adhere today, here tomorrow: oral bacterial adherence. J Bacteriol 175, 3247-3252.
21. Levinthal, C., Signer, E. R. & Fetherolf, K. (1962). Reactivation and hybridization of reduced alkaline phosphatase. Proc Natl Acad Sci USA 48, 1230-1237.
22. Lugtenberg, B., Meijers, J., Peters, R., van der Hoek, P. & van Alphen, L. (1975). Electrophoretic resolution of the 'major outer membrane protein' of Escherichia coli K12 into four bands. FEBS Lett 58, 254-258.
23. Merck, K. B., de Cock, H., Verheij, H. M. & Tommassen, J. (1997). Topology of the outer membrane phospholipase A of Salmonella typhimurium. J Bacteriol 179, 3443-3450.
24. Moeck, G. S., Bazzaz, B. S., Gras, M. F., Ravi, T. S., Ratcliffe, M. J. & Coulton, J. W. (1994). Genetic insertion and exposure of a reporter epitope in the ferrichrome-iron receptor of Escherichia coli K-12. J Bacteriol 176, 4250-4259.
25. Montal, M. (1996). Protein folds in channel structure. Curr Opin Struct Biol 6, 499-510.
26. Nakae, T. (1976). Identification of the outer membrane protein of E. coli that produces transmembrane channels in reconstituted vesicle membranes. Biochem Biophys Res Commun 71, 877-884.
27. Newton, S. M., Klebba, P. E., Michel, V., Hofnung, M. & Charbit, A. (1996). Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model. J Bacteriol 178, 3447-3456.
28. Ohmori, H. (1994). A new method for strand discrimination in sequence-directed mutagenesis. Nucleic Acids Res 22, 884-885.
29. Snijders, A., Benaissa Trouw, B. J., Oosterlaken, T. A. & 7 other authors (1991). Identification of linear epitopes on Semliki Forest virus E2 membrane protein and their effectiveness as a synthetic peptide vaccine. J Gen Virol 72, 557-565.
30. Struyvé, M., Visser, J., Adriaanse, H., Benz, R. & Tommassen, J. (1993). Topology of PhoE porin: the 'eyelet' region. Mol Microbiol 7, 131-140.
31. Sukhan, A. & Hancock, R. E. (1995). Insertion mutagenesis of the Pseudomonas aeruginosa phosphate-specific porin OprP. J Bacteriol 177, 4914-4920.
32. Taylor, I. M., Harrison, J. L., Timmis, K. N. & O'Connor, C. D. (1990). The TraT lipoprotein as a vehicle for the transport of foreign antigenic determinants to the cell surface of Escherichia coli K12: structure-function relationships in the TraT protein. Mol Microbiol 4, 1259-1268.
33. Tommassen, J. & Lugtenberg, B. (1984). Amino terminus of outer membrane PhoE protein: localization by use of a bla-phoE hybrid gene. J Bacteriol 157, 327-329.
34. Tommassen, J., van Tol, H. & Lugtenberg, B. (1983). The ultimate localization of an outer membrane protein of Escherichia coli K-12 is not determined by the signal sequence. EMBO J 2, 1275-1279.
35. Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76, 4350-4354.
36. Van Gelder, P., Saint, N., Phale, P., Eppens, E. F., Prilipov, A., van Boxtel, R., Rosenbusch, J. P. & Tommassen, J. (1997). Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: role of charged residues. J Mol Biol 269, 468-472.
37. Weiss, M. S., Abele, U., Weckesser, J., Welte, W., Schiltz, E. & Schulz, G. E. (1991). Molecular architecture and electrostatic-properties of a bacterial porin. Science 254, 1627-1630.
38. Welte, W., Nestel, U., Wacker, T. & Diederichs, K. (1995). Structure and function of the porin channel. Kidney Int 48, 930-940.
39. Zimmermann, W. & Rosselet, A. (1977). Function of the outer membrane of Escherichia coli as a permeability barrier to betalactam antibiotics. Antimicrob Agents Chemother 12, 368-372.