Human cytomegalovirus replicates abortively in polymorphonuclear leukocytes after transfer from infected endothelial cells via transient microfusion events

Journal of Virology

Volumn 74, Issue 12, 2000, Pages 5629-5638

  Gerna, Giuseppe ^a,e   Percivalle, Elena ^a   Baldanti, Fausto ^a,b   Sozzani, Silvano ^c   Lanzarini, Paolo ^d   Genini, Emilia ^a   Lilleri, Daniele ^a   Revello, Maria Grazia ^a  

^a Servizio di Neuroradiologia   (Italy)

^b Laboratori Sperimentali di Ricerca   (Italy)

^c MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

^d Ist di Clin delle Malat Infettive   (Italy)

^e FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CULTURE; CYTOMEGALOVIRUS INFECTION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; IMMUNOHISTOCHEMISTRY; NEUTROPHIL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; VIRUS PATHOGENESIS; VIRUS REPLICATION;

CELL FUSION; CELL NUCLEUS; CELLS, CULTURED; COCULTURE TECHNIQUES; CYCLOHEXIMIDE; CYTOMEGALOVIRUS; DACTINOMYCIN; DNA, VIRAL; ENDOCYTOSIS; ENDOTHELIUM, VASCULAR; FIBROBLASTS; FOSCARNET; HUMANS; KINETICS; LUNG; NEUTROPHILS; PROTEIN BIOSYNTHESIS; RNA, VIRAL; TRANSCRIPTION, GENETIC; UMBILICAL VEINS; VASCULAR CELL ADHESION MOLECULE-1; VIRAL LOAD; VIRAL PROTEINS; VIRUS REPLICATION;

EID: 0034041719     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.12.5629-5638.2000     Document Type: Article

References (37)

1. Bitsch, A., H. Kirchner, R. Dupke, and G. Bein. 1993. Cytomegalovirus transcripts in peripheral blood leukocytes of actively infected transplant patients detected by reverse-transcription-polymerase chain reaction. J. Infect. Dis. 167:740-743.
2. Blok, M. J., V. J. Goossens, S. J. V. Vanherle, B. Top, N. Tacken, J. M. Middeldorp, M. H. L. Christiaans, J. P. van Hooff, and C. A. Bruggeman. 1998. Diagnostic value of monitoring human cytomegalovirus late pp67 mRNA expression in renal-allograpt recipients by nucleic acid-sequence based amplification. J. Clin. Microbiol. 36:1341-1346.
3. Boivin, G., J. Handfield, E. Toma, R. Lalonde, and M. G. Bergeron. 1999. Expression of the late cytomegalovirus (CMV) pp150 transcript in leukocytes of AIDS patients is associated with a high viral DNA load in leukocytes and presence of CMV DNA in plasma. J. Infect. Dis. 179:1101-1107.
4. Brown, J. M., H. Kaneshima, and E. S. Mocarski. 1995. Dramatic interstrain differences in the replication of human cytomegalovirus in SCID-hu mice. J. Infect. Dis. 171:1599-1603.
5. Butini, L., A. R. De Fougerolles, M. Vaccarezza, C. Graziosi, D. I. Cohen, M. Montroni, T. A. Springer, G. Pantaleo, and A. S. Fauci. 1994. Intracellular adhesion molecules (ICAM)-1, ICAM-2, and ICAM-3 function as counter-receptor for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation. Eur. J. Immunol. 24:2191-2195.
6. Cha, T.-H., E. Tom, G. W. Kemble, G. M. Duke, E. S. Mocarski, and R. S. Spaete. 1996. Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J. Virol. 70:78-83.
7. Cybulsky, M. I., J. W. U. Fries, A. J. Williams, P. Sultan, R. Eddy, M. Byers, T. Shows, M. A. Gimbrone, Jr., and T. Collins. 1991. Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene. Proc. Natl. Acad. Sci. USA 88:7859-7863.
8. Dianzani, F., O. Scheglovitova, M. Gentile, V. Scanio, C. Barresi, B. Ficociello, F. Bianchi, D. Fiumara, and M. R. Capobianchi. 1996. Interferon gamma stimulates cell-mediated transmission of HIV tipe 1 from abortively infected endothelial cells. AIDS Res. Hum. Retrovir. 12:621-627.
9. Gallina, A., E. Percivalle, L. Simoncini, M. G. Revello, G. Gerna, and G. Milanesi. 1996. Human cytomegalovirus pp65 lower matrix phosphoprotein harbours two transplantable nuclear localization signals. J. Gen. Virol. 77: 1151-1157.
10. Gerna, G., D. Zipeto, E. Percivalle, M. Parea, M. G. Revello, R. Maccario, G. Peri, and G. Milanesi. 1992. Human cytomegalovirus infection of the major leukocyte subpopulations and evidence for initial viral replication in polymorphonuclear leukocytes from viremic patients. J. Infect. Dis. 166:1236-1244.
11. Gerna, G., E. Percivalle, M. Torsellini, and M. G. Revello. 1998. Standardization of the human cytomegalovirus antigenemia assay by means of in vitro generated pp65-positive peripheral blood polymorphonuclear leukocytes. J. Clin. Microbiol. 36:3585-3589.
12. Gerna, G., F. Baldanti, J. M. Middeldorp, M. Furione, M. Zavattoni, D. Lilleri, and M. G. Revello. 1999. Clinical significance of expression of human cytomegalovirus pp67 late transcript in heart, lung and bone marrow transplant recipients as determined by nucleic acid sequence-based amplification. J. Clin. Microbiol. 37:902-911.
13. Gerna, G., M. Furione, F. Baldanti, and A. Sarasini. 1994. Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J. Clin. Microbiol. 32:2709-2717.
14. Gerna, G., M. G. Revello, E. Percivalle, and F. Morini. 1992. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J. Clin. Microbiol. 30:1232-1237.
15. Gerna, G., M. G. Revello, E. Percivalle, M. Zavattoni, M. Parea, and M. Battaglia. 1990. Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J. Clin. Microbiol. 28:2681-2688.
16. Gozlan, J., J. M. Salord, C. Chouaïd, C. Duvivier, O. Picard, M. C. Mejohas, and J. C. Petit. 1993. Human cytomegalovirus (HCMV) late mRNA detection in peripheral blood of AIDS patients: diagnostic value for HCMV disease compared with those of viral culture and HCMV DNA detection. J. Clin. Microbiol. 31:1943-1945.
17. Gozlan, J., J. P. Laporte, S. Lesage, M. Labopin, A. Najman, N. C. Gorin, and J. C. Petit. 1996. Monitoring of cytomegalovirus infection and disease in bone marrow recipients by reverse trascription-PCR and blood and urine culture. J. Clin. Microbiol. 34:2085-2088.
18. Grefte, A., M. C. Harmsen, M. van der Giessen, S. Knollema, W. J. van Son, and T. H. The. 1994. Presence of human cytomegalovirus (HCMV) immediate-early mRNA hut not pp UL83 (lower matrix protein pp65) mRNA in polymorphonuclear and mononuclear leukocytes during active HCMV infection. J. Gen. Virol. 75:1989-1998.
19. Grundy, J. E., K. M. Lawson, L. P. MacCormac, J. M. Fletcher, and K. L. Yong. 1998. Cytomegalovirus-infected endothelial cells recruit neutrophils by the secretion of C-X-C chemokines and transmit virus by direct neutrophil-endothelial cell contact and during neutrophil transendothelial migration. J. Infect. Dis. 177:1465-1474.
20. Hession, K., R. Tizard, C. Vassallo, S. B. Schiffer, D. Goff, P. Moy, G. Chi-Rosso, S. Luhowski, R. Lobb, and L. Osborn. 1991. Cloning of an alternate form of vascular cell adhesion molecule-1 (VCAM1). J. Biol. Chem. 266: 6682-6685.
21. Jaroszeski, M. J., R. Gilbert, and R. Heller. 1994. Detection and quantitation of cell-cell electrofusion products by flow cytometry. Anal. Biochem. 216: 271-275.
22. Lam, K. M. C., N. Oldenburg, M. A. Khan, V. Gaylore, G. W. Mikhail, P. D. Stroual, J. M. Middeldorp, N. Banner, and M. Yacoub. 1998. Significance of reverse transcription polymerase chain reaction in the detection of human cytomegalovirus gene transcripts in thoracic organ transplant recipients. J. Heart Lung Transplant 17:555-565.
23. Meyer, T., U. Reischl, H. Wolf, C. Schuller, and R. Arndt. 1994. Identification of active cytomegalovirus infection by analysis of immediate-early, early and late transcripts in peripheral blood cells of immunodeficient patients. Mol. Cell. Probes 8:261-271.
24. Meyer-König, U., A. Serr, D. van Laer, G. Kirste, C. Wolff, O. Haller, D. Neuman-Haefelin, and F. T. Hufert 1995. Human cytomegalovirus immediate-early and late transcripts in peripheral blood leukocytes: diagnostic value in renal transplant recipients. J. Infect. Dis. 171:705-709.
25. Nelson, P. N., B. K. Kawal, Y. S. Boriskin, K. E. Mathers, R. L. Powles, H. M. Steel, Y. S. Tryhorn, P. D. Butcther, and J. C. Booth. 1996. A polymerase chain reaction to detect a spliced late transcript of human cytomegalovirus in the blood of bone marrow transplant recipients. J. Virol. Methods 56:139-148.
26. Randhawa, P. S., R. Manez, B. Frye, and G. D. Ehrlich. 1994. Circulating immediate-early mRNA in patients with cytomegalovirus infections after solid organ transplantation. J. Infect. Dis. 170:1264-1267.
27. Revello, M. G., E. Percivalle, E. Arbustini, R. Pardi, S. Sozzani, and G. Gerna. 1998. In vitro generation of human cytomegalovirus pp65 antigenemia, viremia, and leukoDNAemia. J. Clin. Investig. 101:2686-2692.
28. Revello, M. G., E. Percivalle, M. Zavattoni, M. Parea, P. Grossi, and G. Gerna. 1989. Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. J. Med. Virol. 29:88-93.
29. Saltzmann, R. L., M. R. Quirk, and M. C. Jordan. 1988. Disseminated cytomegalovirus infection. Molecular analysis of virus and leukocyte interactions in viremia. J. Clin. Investig. 81:75-81.
30. Schmolke, S., P. Drescher, G. Jahn, and B. Plachter. 1995. Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport. J. Virol. 69:1071-1078.
31. Stanier, P., D. L. Taylor, A. D. Kitchen, N. Wales, Y. Tryhorn, and A. S. Tyms. 1989. Persistence of cytomegalovirus in mononuclear cells in peripheral blood from blood donors. Br. Med. J. 299:897-898.
32. Taylor-Wiedeman, J., G. P. Hayhurst, J. G. P. Sissons, and J. H. Sinclair. 1993. Polymorphonuclear cells are not sites of persistence of human cytomegalovirus in healthy individuals. J. Gen. Virol. 74:265-268.
33. Taylor-Wiedeman, J., J. G. P. Sissons, L. K. Borysiewicz, and J. H. Sinclair. 1991. Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells. J. Gen. Virol. 72:2059-2064.
34. van der Bij, W., J. Schirm, R. Torensma, W. J. van Son, A. M. Tegzess, and T. H. The. 1988. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J. Clin. Microbiol. 26:2531-2535.
35. Velzing, J., P. H. Rothbarth, A. C. M. Kroes, and W. G. V. Quint. 1994. Detection of cytomegalovirus mRNA and DNA encoding the immediateearly gene in peripheral blood leukocytes from immunocompromised patients. J. Med. Virol. 42:164-169.
36. von Laer, D., A. Serr, U. Meyer-König, G. Kirste, F. T. Hufert, and O. Haller. 1995. Human cytomegalovirus immediate early and late transcripts are expressed in all major leukocyte populations in vivo. J. Infect. Dis. 172:365-370.
37. Zaia, J. A., S. J. Forman, M. T. Gallagher, E. Vanderwal-Urbina, and K. G. Blume. 1984. Prolonged human cytomegalovirus viremia following bone marrow transplantation. Transplantation 37:315-317.