In vitro generation of human cytomegalovirus pp65 antigenemia, viremia, and leukoDNAemia

Journal of Clinical Investigation

Volumn 101, Issue 12, 1998, Pages 2686-2692

  Revello, Maria Grazia ^a,e   Percivalle, Elena ^a   Arbustini, Eloisa ^b   Pardi, Ruggero ^c   Sozzani, Silvano ^d   Gerna, Giuseppe ^a  

^a Servizio di Virologia   (Italy)

^b Istituto di Anatomia Patologica   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

^d MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

^e FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

Author keywords

Cocultivation; Endothelial cells; Fibroblasts; Immunocompromised patients; Polymorphonuclear leukocytes

Indexed keywords

VIRUS DNA;

ARTICLE; COCULTURE; CYTOMEGALOVIRUS INFECTION; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; IMMUNE DEFICIENCY; NEUTROPHIL; PRIORITY JOURNAL; VIREMIA; VIRUS REPLICATION; VIRUS STRAIN;

EID: 0032526099     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI1633     Document Type: Article

References (26)

1. Van der Bij, W., R. Torensma, W. Van Son, J. Anema, J. Schirm, A.M. Tegzess, and T.H. The. 1998. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leukocytes. J. Med. Virol. 25:174-188.
2. Revello, M.G., E. Percivalle, A. Di Matteo, F. Morini, and G. Gerna. 1992. Nuclear expression of the lower matrix protein of human cytomegalovirus in peripheral blood leukocytes of immunocompromised viraemic patients. J. Gen. Virol. 73:437-442.
3. Gerna, G., M.G. Revello, E. Percivalle, M. Zavattoni, M. Parea, and M. Battaglia. 1990. Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J. Clin. Microbiol. 28:2681-2688.
4. Turtinen, L.W., R. Saltzman, M.C. Jordan, and A.T. Hausse. 1987. Interaction of human cytomegalovirus with leukocytes: in vivo analysis by in situ hybridization. Microb. Pathog. 3:287-297.
5. Gerna, G., D. Zipeto, E. Percivalle, M. Parea, M.G. Revello, R. Maccario, G. Peri, and G. Milanesi. 1992. Human cytomegalovirus infection of the major leukocyte subpopulations and evidence for initial viral replication in polymorphonuclear leukocytes from viremic patients. J. Infect. Dis. 166:1236-1244.
6. Percivalle, E., M.G. Revello, L. Vago, F. Morini, and G. Gerna. 1993. Circulating endothelial giant cells permissive for human cytomegalovirus (HCMV) are detected in disseminated HCMV infections with organ involvement. J. Clin. Invest. 92:663-670.
7. Grefte, A., M. van der Giessen, W. van Son, and T.H. The. 1993. Circulating cytomegalovirus (CMV)-infected endothelial cells in patients with an active CMV infection. J. Infect. Dis. 167:270-277.
8. Vaccaro, P.S., L.B. Joseph, L. Titterington, and R.E. Stephens. 1987. Methods for the initiation and maintenance of human endothelial cell culture. Vasc. Surg. 21:391-400.
9. Chen, T.R. 1977. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp. Cell Res. 104:255-262.
10. Gerna, G., M.G. Revello, M. Palla, E. Percivalle, and M. Torsellini. 1992. Microneutralization as a reference method for selection of the cut-off of an enzyme-linked immunosorbent assay for detection of IgG antibody to human cytomegalovirus. Microbiologica (Pavia). 15:177-182.
11. Gerna, G., M.G. Revello, E. Percivalle, and F. Morini. 1992. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J. Clin. Microbiol. 30:1232-1237.
12. Springer, T.A. 1994. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 76:301-314.
13. Gerna, G., F. Baldanti, A. Sarasini, M. Furione, E. Percivalle, M.G. Revello, D. Zipeto, and D. Zella. 1994. Effect of foscarnet induction treatment on quantitation of human cytomegalovirus (HCMV) DNA in peripheral blood polymorphonuclear leukocytes and aqueous humor of AIDS patients with HCMV retinitis. The Italian Foscarnet Study Group. Antimicrob. Agents Chemother. 38:38-44.
14. Span, A.H.M., C.P.A. Van Boven, and C.A. Bruggeman. 1989. The effect of cytomegalovirus infection on the adherence of polymorphonuclear leukocytes to endothelial cells. Eur. J. Clin. Invest. 19:542-548.
15. Span, A.H.M., M.C.E. Van Dam-Mieras, W. Mullers, J. Endert, A.D. Muller, and C.A. Bruggeman. 1991. The effect of virus infection on the adherence of leukocytes or platelets to endothelial cells. Eur. J. Clin. Invest. 21:331-338.
16. Sedmak, D.D., D.A. Knight, N.C. Vonk, and J.W. Waldman. 1994. Divergent patterns of ELAM-1. ICAM-1. and VCAM-1 expression on cytomegalovirus-infected endothelial cells. Tranplantation. 12:1379-1385.
17. Grundy, J.E., and K.L. Downes. 1993. Up-regulation of LFA-3 and ICAM-1 on the surface of fibroblasts infected with cytomegalovirus. Immunology. 78:405-412.
18. Waldman, W.J., D.A. Knight, E.H. Huang, and D.D. Sedmak. 1995. Bidirectional transmission of infectious cytomegalovirus between monocytes and vascular endothelial cells: an in vitro model. J. Infect. Dis. 171:263-272.
19. Elek, S.D., and H. Stern. 1474. Development of a vaccine against mental retardation caused by cytomegalovirus infection in utero. Lancet. 1:1-5.
20. Plotkin, S.A., J. Farquhar, and E. Hornberger. 1976. Clinical trials of immunization with the Towne 125 strain of cytomegalovirus. J. Infect. Dis. 134: 470-475.
21. Waldman, W.J., W.H. Roberts, D.H. Davis, M.V. Williams, D.D. Sedmak, and R.E. Stephens. 1991. Preservation of natural endothelial cytopathogenicity of cytomegalovirus by propagation in endothelial cells. Arch. Virol. 117: 143-164.
22. Radsak, K., R. Fuhrmann, R.P. Franke, D. Schneider, A. Kollert, K.H. Brucher, and D. Drenckhahan. 1989. Induction by sodium butyrate of cytomegalovirus replication in human endothelial cells. Arch. Virol. 107:151-158.
23. Friedman, H.M., E.J. Makarak, R.R. MacGregor, J. Wolfe, and N.A. Kefalides. 1981. Virus infection of endothelial cells. J. Infect. Dis. 143:266-273.
24. Brown, J.M., H. Kaneshima, and E.S. Mocarski. 1995. Dramatic interstrains differences in the replication of human cytomegalovirus in SCID-humice. J. Infect. Dis. 171:1599-1603.
25. Cha, T.-H., E. Tom, G.W. Kemble, G.M. Duke, E.S. Mocarski, and R.S. Spaete. 1996. Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains. J. Virol. 70:78-83.
26. Gerna, G., M. Furione, F. Baldanti, and A. Sarasini. 1994. Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J. Clin. Microbiol. 32:2709-2717.