pH heterogeneity at intracellular and extracellular plasma membrane sites in HT29-C1 cell monolayers

American Journal of Physiology - Cell Physiology

Volumn 278, Issue 5 47-5, 2000, Pages

  Maouyo, Djikolngar ^a   Chu, Shaoyou ^b   Montrose, Marshall H ^b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Ammonium; Carboxyseminaphthorhodofluor 1; Colon; Epithelium; Laser scanning confocal microscopy; NHE1; NHE2; Polarity; Propionate; Short chain fatty acid

Indexed keywords

AMMONIUM CHLORIDE; CARBOXYSEMINAPHTHORHODOFLUOR 1; NAPHTHALENE DERIVATIVE; PROPIONIC ACID; SHORT CHAIN FATTY ACID; UNCLASSIFIED DRUG;

ACIDIFICATION; ALKALINIZATION; APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; CELL PH; CELL POLARITY; CELL STRAIN HT29; CONTROLLED STUDY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTON SODIUM EXCHANGE;

EID: 0034036287     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.2000.278.5.c973     Document Type: Article

References (11)

1. Abrahamse SL, Pool-Zobel BL, and Rechkemmer G. Potential of short-chain fatty acids to modulate the induction of DNA damage and changes in the intracellular calcium concentration by oxidative stress in isolated rat distal colon cells. Carcinogenesis 20: 629-634, 1999.
2. Allen A, Hutton D, McQueen S, and Garner A. Dimensions of gastroduodenal surface pH gradients exceed those of adherent mucus gel layers. Gastroenterology 85: 463-465, 1983.
3. Binder HJ and Mehta P. Short-chain fatty acids stimulate active sodium and chloride absorption in vitro in the rat distal colon. Gastroenterology 96: 989-996, 1989.
4. Bugaut M. Occurrence, absorption, and metabolism of short-chain fatty acids in the digestive tract of mammals. Comp Biochem Physiol B Biochem Mol Biol 86: 439-472, 1987.
5. Chatton JY and Spring KR. Acidic pH of the lateral intercellular spaces of MDCK cells cultured on permeable supports. J Membr Biol 140: 89-99, 1994.
6. Cherbut C, Aube AC, Blottiere HM, Pacaud P, Scarpignato C, and Galmiche JP. In vitro contractile effects of short-chain fatty acids in the rat terminal ileum. Gut 38: 53-58, 1996.
7. Chu S, Brownell WE, and Montrose MH. Quantitative confocal imaging along the crypt-to-surface axis of colonic crypts. Am J Physiol Cell Physiol 269: C1557-C1564, 1995.
8. Chu S and Montrose MH. Extracellular pH regulation in microdomains of colonic crypts: effects of short-chain fatty acids. Proc Natl Acad Sci USA 92: 3303-3307, 1995.
9. +-independent short-chain fatty acid transporter contributes to intracellular pH regulation in murine colonocytes. J Gen Physiol 105: 589-615, 1995.
10. Chu S and Montrose MH. Non-ionic diffusion and carrier-mediated transport drive extracellular pH regulation of mouse colonic crypts. J Gen Physiol 494: 783-793, 1996.
11. Chu S, Tanaka S, Kaunitz JD, and Montrose MH. Dynamic regulation of gastric surface pH by luminal pH. J Clin Invest 103: 605-612, 1999.