Isolation and characterization of polyadenylation complexes assembled in vitro

RNA

Volumn 6, Issue 5, 2000, Pages 768-777

  Veraldi, Kristen L ^a   Edwalds Gilbert, Gretchen ^a,c   Macdonald, Clinton C ^b   Wallace, A Michelle ^b   Milcarek, Christine ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^c CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

Plasma cells; Pre mRNA 3' end processing

Indexed keywords

MESSENGER RNA PRECURSOR; POLYADENYLATED RNA;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CONTROLLED STUDY; CROSS LINKING; IMMUNOPRECIPITATION; MOUSE; MYELOMA CELL; NONHUMAN; POINT MUTATION; POLYADENYLATION; PRIORITY JOURNAL; RNA CLEAVAGE; RNA PROCESSING; RNA PURIFICATION; RNA SEQUENCE; SILVER STAINING; WESTERN BLOTTING;

ANIMALS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; KINETICS; MICE; MULTIPLE MYELOMA; MUTATION; POLY A; RNA PRECURSORS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, NEOPLASM; TUMOR CELLS, CULTURED;

ANIMALIA; MAMMALIA;

EID: 0034032602     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S135583820099246X     Document Type: Article

References (42)

1. Bennett M, Pinol-Roma S, Staknis D, Dreyfuss G, Reed R. 1992. Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro. Mol Cell Biol 12:3165-3175.
2. Beyer K, Dandekar T, Keller W. 1997. RNA ligands selected by cleavage stimulation factor contain distinct sequence motifs that function as downstream elements in 3′-end processing of pre-mRNA. J Biol Chem 272:26769-26779.
3. Bienroth S, Wahle E, Suter-Crazzolara C, Keller W. 1991. Purification of the cleavage and polyadenylation factor involved in the 3′-processing of messenger RNA precursors. J Biol Chem 266:19768-19776.
4. Chen F, MacDonald C, Wilusz J. 1995. Cleavage site determinants in the mammalian polyadenylation signal. Nucleic Acids Res 23:2614-2620.
5. Christofori G, Keller W. 1989. Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro. Mol Cell Biol 9:193-203.
6. Dantonel J-C, Murthy K, Manley J, Tora L. 1997. Transcription factor TFIID recruits factor CPSF for formation of 3′ end of mRNA. Nature 389:399-402.
7. Dignam J, Lebovitz R, Roeder R. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11:1475-1489.
8. Edwalds-Gilbert G, Milcarek C. 1995. Regulation of poly(A) site use during mouse B-cell development involves a change in the binding of a general polyadenylation factor in a B-cell stage-specific manner. Mol Cell Biol 15:6420-6429.
9. Edwalds-Gilbert G, Veraldi K, Milcarek C. 1997. Alternative poly(A) site selection in complex transcription units: Means to an end? Nucleic Acids Res 25:2547-2561.
10. Gilmartin G, Nevins J. 1991. Molecular analysis of two poly(A) site-processing factors that determine the recognition and efficiency of cleavage of the pre-mRNA. Mol Cell Biol 11:2432-2438.
11. Grabowski PJ, Sharp PA. 1986. Affinity chromatography of splicing complexes: U2, U5, and U4+U6 small ribonucleoprotein particles in the spliceosome. Science 233:1294-1299.
12. Gunderson S, Polycarpou-Schwartz M, Mattaj I. 1998. U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase. Mol Cell 1:255-264.
13. Gunderson S, Vagner S, Polycarpou-Schwartz M, Mattaj I. 1997. Involvement of the carboxyl terminus of vertebrate poly(A) polymerase in U1A autoregulation and the coupling of splicing and polyadenylation. Genes & Dev 11:761-773.
14. Jenny A, Hauri H, Keller W. 1994. Characterization and polyadenylation specificity factor and cloning of its 100-kilodalton subunit. Mol Cell Biol 14:8183-8190.
15. Keller W. 1995. No end yet to messenger RNA 3′ processing. Cell 81:829-832.
16. Keller W, Bienroth S, Lang KM, Christofori G. 1991. Cleavage and polyadenylation factor CPF specifically interacts with the pre-mRNA 3′ processing signal AAUAAA. EMBO J 10:4241-4249.
17. Lutz C, Murthy K, Schek N, O'Conner J, Manley J, Alwine J. 1996. Interaction between the U1 snRNP-A protein and the 160-kD subunit of cleavage-polyadenylation specificity factor increases polyadenylation efficiency in vitro. Genes & Dev 10:325-337.
18. MacDonald C, Wilusz J, Shenk T. 1994. The 64 kDa subunit of the CstF polyadenylation factor binds to pre-mRNAs downstream of cleavage site and influences cleavage site location. Mol Cell Biol 14:6647-6654.
19. o to S phase transition. Proc Natl Acad Sci USA 95:11095-11100.
20. Matis SA, Martincic K, Milcarek C. 1996. B-lineage regulated polyadenylation occurs on weak poly(A) sites regardless of sequence composition at the cleavage and downstream regions. Nucleic Acids Res 24:4684-4692.
21. Matthews HR. 1968. The application of Cerenkov counting to column chromatography of 32P labelled substances. J Chromatogr 36: 302-308.
22. McCracken S, Fong N, Yankulov K, Ballantyne S, Pan G, Greenblatt J, Patterson SD, Wickens M, Bentley DL. 1997. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 385:357-361.
23. Milcarek C, Hartman M, Croll S. 1996. Changes in abundance of IgG 2a mRNA in the nucleus and cytoplasm of a murine B-lymphoma before and after fusion to a myeloma cell. Mol Immunol 33:691-701.
24. Moore C, Sharp P. 1985. Accurate cleavage and polyadenylation of exogenous RNA substrate. Cell 41:845-855.
25. Moore CL, Skolnik-David H, Sharp PA. 1988. Sedimentation analysis of polyadenylation specific complexes. Mol Cell Biol 8:226-233.
26. Murthy K, Manley J. 1992. Characterization of the multisubunit cleavage-polyadenylation specificity factor from calf thymus. J Biol Chem 267:14804-14811.
27. Murthy KGK, Manley JL 1995. The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3′-end formation. Genes & Dev 9:2672-2683.
28. Poehling H-M, Neuhoff V. 1981. Visualization of proteins with a silver "stain": A critical analysis. Electrophoresis 2:141-147.
29. Reed R. 1990. Protein composition of mammalian spliceosomes assembled in vitro. Proc Natl Acad Sci USA 87:8031-8035.
30. m involved in the 3′ end processing of messenger RNA precursors. J Biol Chem 271:6107-6113.
31. Rüegsegger U, Blank D, Keller W. 1998. Human pre-mRNA Cleavage Factor Im is related to spliceosomal SR proteins and can be reconstituted in vitro from recombinant subunits. Mol Cell 1:243-253.
32. Stefano J, Adams D. 1988. Assembly of a polyadenylation-specific 25S ribonucleoprotein complex in vitro. Mol Cell Biol 8:2052-2062.
33. Takagaki Y, MacDonald C, Shenk T, Manley J. 1992. The human 64-kDa polyadenylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs. Proc of the Natl Acad Sci USA 89:1403-1407.
34. Takagaki Y, Manley JL. 1997. RNA recognition by the human polyadenylation factor CstF. Mol Cell Biol 17:3907-3914.
35. Takagaki Y, Manley JL 2000. Complex protein interactions within the human polyadenylation machinery identify a novel component. Mol Cell Biol 20:1515-1525.
36. Takagaki Y, Ryner L, Manley J. 1988. Separation and characterization of a poly(A) polymerase and a cleavage/specificity factor required for pre-mRNA polyadenylation. Cell 52:731-742.
37. Wahle E. 1991. A novel polyA-binding protein acts as a specificity factor in the second phase of mRNA polyadenylation. Cell 66:759-768.
38. Wahle E, Keller W. 1992. The biochemistry of 3′-end cleavage and polyadenylation of messenger RNA precursors. Annu Rev Biochem 61:419-440.
39. r protein of the cleavage stimulation factor are expressed in mouse male germ cells. Proc Natl Acad Sci USA 96:6763-6768.
40. Wilusz J, Shenk T. 1990. A uridylate tract mediates efficient hnRNP C protein-RNA crosslinking and functionally substitutes for the downstream element of the polyadenylation signal. Mol Cell Biol 10:6397-6407.
41. Wilusz J, Shenk T, Takagaki Y, Manley J. 1990. A multicomponent complex is required for the AAUAAA-dependent cross-linking of a 64-kilodalton protein to polyadenylation substrates. Mol Cell Biol 10:1244-1248.
42. Zhao J, Hyman L, Moore C. 1999. Formation of mRNA 3′ ends in eukaryotes: Mechanism, regulation, and interrelationships with other steps in mRNA synthesis. Microbiol Mol Biol Rev 63:405-445.