PEST sequences in cAMP-dependent protein kinase subunits of the aquatic fungus Blastocladiella emersonii are necessary for in vitro degradation by endogenous proteases

Molecular Microbiology

Volumn 36, Issue 4, 2000, Pages 926-939

  Borges, Ana Claudia Cantisani ^a   Gomes, Suely Lopes ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; MUTANT PROTEIN; PROTEIN SUBUNIT; PROTEINASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BLASTOCLADIELLA; CONTROLLED STUDY; ENZYME KINETICS; ENZYME STABILITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; REGULATORY MECHANISM; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BLASTOCLADIELLA; CATALYTIC DOMAIN; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENDOPEPTIDASES; GENE EXPRESSION; HOLOENZYMES; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC;

BLASTOCLADIELLA EMERSONII; ESCHERICHIA COLI; FUNGI;

EID: 0034031548     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01912.x     Document Type: Article

References (43)

1. Behrens, M.M., and Maia, J.C.C. (1986) Differentiation specific activation of cyclic AMP-dependent protein kinase in Blastocladiella emersonii. Biochem Intern 12: 503-512.
2. Bencina, M., Panneman, H., Ruijter, G.J., Legisa, M., and Visser, J. (1997) Characterization and overexpression of the Aspergillus niger gene encoding the cAMP-dependent protein kinase catalytic subunit. Microbiology 143: 1211-1220.
3. Bradford, M.M. (1976) A rapid and sensitive method for the quantation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
4. Brochetto-Braga, M.R., Gomes, S.L, and Maia, J.C.C. (1982) Studies on the adenosine 3′,5′-monophosphate-dependent protein kinase of Blastocladiella emersonii. Arch Biochem Biophys 217: 295-304.
5. 2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle. Proc Natl Acad Sci USA 79: 6128-6131.
6. Etchebehere, L.C., Van Bemmelen, M.X.P., Anjard, C., Traincard, F., Assemat, K., Reymond, C., et al. (1997) The catalytic subunit of Dictyostelium cAMP-dependent protein kinase. Role of the N-terminal domain and of the C-terminal residues in catalytic activity and stability. Eur J Biochem 248: 820-826.
7. Eto, A., Akita, Y., Saido, T.C., Susuki, K., and Kawashima, S. (1995) The role of the calpain-calpastatin system in thyrotropin-releasing hormone-induced selective down-regulation of a protein kinase C isozyme, nPKC epsilon, in rat pituitary GH4C1 cells. J Biol Chem 270: 25115-25120.
8. Glotzer, M., Murray, A.W., and Kirschner, M.W. (1991) Cyclin is degraded by the ubiquitin pathway. Nature 349: 132-138.
9. Gomes, S.L., Juliani, M.H., Maia, J.C.C., and Rangel-Aldao, R. (1983) Autophosphorylation and rapid dephosphorylation of the cAMP-dependent protein kinase from Blastocladiella emersonii zoospores. J Biol Chem 258: 6972-6978.
10. Gottesman, S. (1999) Regulation by proteolysis: developmental switches. Curr Opin Microbiol 2: 142-147.
11. Hemmings, B.A. (1986) cAMP mediated proteolysis of the catalytic subunit of cAMP-dependent protein kinase. FEBS Microbiol Lett 196: 126-130.
12. Juliani, M.H., Brochetto, M., and Maia, J.C.C. (1979) Changes in cyclic AMP binding and protein kinase activities during growth and differentiation of Blastociadiella emersonii. Cell Differ 8: 421-430.
13. King, R.W., Deshaies, R.J., Peters, J.M., and Kirschner, M.W. (1996) How proteolysis drives the cell cycle. Science 274: 1652-1659.
14. Kunkel, T.A. (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci USA 82: 488-492.
15. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 226: 680-685.
16. Lovett, J.S. (1975) Growth and differentiation of the water mold Blastocladiella emersonii. Cytodifferentiation and the role of ribonucleic acid and protein synthesis. Bacteriol Rev 34: 345-404.
17. Mann, S.K., Yonemoto, W.M., Taylor, S.S., and Firtel, R.A. (1992) DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase. Proc Natl Acad Sci USA 89: 10701-10705.
18. Marques, M.V., and Gomes, S.L. (1992) Cloning and structural analysis of the gene for the regulatory subunit of cAMP-dependent protein kinase in Blastocladiella emersonii. J Biol Chem 74: 5463-5467.
19. Marques, M.V., Juliani, M.H., Maia, J.C.C., and Gomes, S.L. (1989) Developmental regulation of expression of the regulatory subunit of the cAMP-dependent protein kinase of Blastocladiella emersonii. Eur J Biochem 178: 803-810.
20. Marques, M.V., Borges, A.C.C., de Oliveira, J.C.F., and Gomes, S.L. (1992) Coordinate pretranslational control of cAMP-dependent protein kinase subunit expression during development in Blastocladiella emersonii. Dev Biol 149: 432-439.
21. Matsudaira, P. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 26: 10035-10038.
22. de Oliveira, J.C.F., Borges, A.C.C., Marques, M.V., and Gomes, S.L. (1994) Cloning and characterization of the gene for the catalytic subunit of cAMP-dependent protein kinase in the aquatic fungus Blastocladiella emersonii. Eur J Biochem 219: 555-562.
23. de Oliveira, J.C.F., Marques, M.V., and Gomes, S.L. (1997) Protein factors in Blastocladiella emersonii cells extracts recognize similar sequence elements in the promoters of the genes encoding cAMP-dependent protein kinase subunits. Mol Gen Genet 255: 495-503.
24. Pearson, W.R., and Lipman, D.J. (1988) Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85: 2444-2448.
25. Rechsteiner, M. (1990) PEST sequences are signals for rapid intracellular proteolysis. Semin Cell Biol 1: 433-440.
26. Rechsteiner, M. (1991) Natural substrates of the ubiquitin proteolytic pathway. Cell 66: 615-618.
27. Rechsteiner, M., and Rogers, A.S. (1996) PEST sequences and regulation by proteolysis. Trends Biochem Sci 21: 267-271.
28. Rogers, S., Rodney, E., and Rechsteiner, M. (1986) Aminoacid sequences common to rapidly degraded proteins: the PEST hypothesis. Science 234: 364-368.
29. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 82: 488-492.
30. Schechter, I., and Berger, A. (1967) On the size of the active site in proteases. I. Papain. Biochem Biophys Res Commun 27: 157-162.
31. Sekhar, K.R., and Freeman. M.L. (1998) PEST sequences in proteins involved in cyclic nucleotide signalling pathways. J Recept Signal Transduct Res 18: 113-132.
32. Soll, D.R., and Sonneborn, D.R. (1971a) Zoospore germination in Blastocladiella emersonii. 3. Structural changes in relation to protein and RNA synthesis. J Cell Sci 9: 679-699.
33. Soll, D.R., and Sonneborn, D.R. (1971b) Zoospore germination in Blastocladiella emersonii: cell differentiation without protein synthesis? Proc Natl Acad Sci USA 68: 459-463.
34. Sorimachi, H., Ishiura, S., and Suzuki, K. (1997) Structure and physiological function of calpains. Biochem J 328: 721-732.
35. Stefani, R.M.P., and Gomes, S.L. (1995) A unique intron-containing hsp70 gene induced by heat shock and during sporulation in the aquatic fungus Blastocladiella emersonii. Gene 152: 19-26.
36. Steinberg, R.A., and Agard, D.A. (1981) Turnover of regulatory subunit of cyclic AMP-dependent protein kinase in S49 mouse lymphoma cells. Regulation by catalytic subunit and analogs of cyclic AMP. J Biol Chem 256: 10731-10734.
37. Studier, W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185: 60-89.
38. Toda, T., Cameron, S., Sass, P., Zoller, M., and Wigler, M. (1987) Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell 50: 277-287.
39. Towbin, H., Sthaelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
40. Vale, V.L., Gomes, S.L., Maia, J.C.C., and Mennucci, L. (1976) Transient cyclic AMP accumulation in germinating zoospores of Blastocladiella emersonii. FEBS Microbiol Lett 67: 189-192.
41. Varshavsky, A. (1992) The N-end rule. Cell 69: 725-735.
42. Yonemoto, W.M., McGlone, M.L., Slice, L.W., and Taylor, S.S. (1991) Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase. Methods Enzymol 200: 581-596.
43. Yonemoto, W., McGlone, M.L., and Taylor, S.S. (1993) N-Myristylation of the catalytic subunit of cAMP-dependent protein kinase conveys structural stability. J Biol Chem 268: 2348-2352.