Microfiltration of protein precipitate from clarified yeast cell homogenate for the recovery of a soluble product

Separation Science and Technology

Volumn 35, Issue 5, 2000, Pages 633-649

  Levesley, J A ^a,b   Seggianni, M ^a   Hoare, M ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b CALLAGHAN INNOVATION   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; M PROTEIN;

CROSSFLOW MICROFILTRATION; PROTEIN;

ARTICLE; CELL LYSATE; HOMOGENATE; MICROFILTRATION; PROTEIN ANALYSIS; SOLUBILITY; THEORY; YEAST;

EID: 0034031022     PISSN: 01496395     EISSN: None     Source Type: Journal    
DOI: 10.1081/SS-100100181     Document Type: Article

References (29)

1. C. S. Parnham III and R. H. Davis, "Protein Recovery from Cell Debris Using Rotary and Tangential Crossflow Microfiltration," Biotechnol. Bioeng., 47, 155-164 (1995).
2. A. I. Clarkson, M. Bulmer, and N. J. Titchener-Hooker, "Pilot-Scale Verification of a Computer-Based Simulation for Fractional Protein Precipitation," Bioprocess Eng., 14(2), 69-80 (1996).
3. K. H. Kroner, H. Schütte, H. Hustedt, and M.-R. Kula, "Crossflow Filtration in Down-stream Processing of Enzymes," Process Biochem., 19(2), 67-74 (1984).
4. A. C. Bentham, M. J. Ireton, M. Hoare, and P. Dunnill, "Protein Precipitate Recovery Using Microporous Membranes," Biotechnol. Bioeng., 31, 984-994 (1988).
5. H. O. Bergmeyer, Methods of Enzymatic Analysis, Verlag Chemie, Weinham, 1983.
6. P. Milburn, J. Bonnerjea, M. Hoare, and P. Dunnill, "Selective Flocculation of Nucleic Acids, Lipids, and Colloidal Particles from a Yeast Cell Homogenate by Polyethyleneimine, and Its Scale Up," Enzyme Microbiol. Technol., 12, 527-532 (1990).
7. G. Belfort, R. H. Davis, and A. L. Zydney, "The Behaviour of Suspensions and Macromolecular Solutions in Crossflow Microfiltration," J. Membr. Sci., 96(1-2), 1-58 (1994).
8. S. M. Forman, F. R. DeBernardez, R. S. Feldberg, and R. W. Swartz, "Crossflow Filtration for the Separation of Inclusion Bodies from Soluble Proteins in Recombinant Escherichia coli Cell Lysate," Ibid., 48, 263-279 (1990).
9. M. C. Porter, "Concentration Polarization with Membrane Ultrafiltration," Ind. Eng. Chem., Prod. Res. Dev., 11(3), 234-248 (1972).
10. C. W. van Oers, M. A. G. Vorstman, W. G. H. M. Muijselgar, and P. J. A. M. Kerkhof, "Unsteady State Flux Behaviour in Relation to the Presence of a Gel Layer," J. Membr. Sci. 73(2-3), 231 (1992).
11. P. Aimar and R. W. Field, "Limiting Flux in Membrane Separations. A Model Based on the Viscosity Dependency of Mass Transfer Coefficient," Chem. Eng. Sci., 47(3), 579-586 (1992).
12. A. G. Fane, "Ultrafiltration, Factors Influencing Flux and Rejection," in Progress in Filtration and Separation, Vol. 4 (R. J. Wakeman, Ed.), Elsevier Science, Amsterdam, 1986.
13. C. A. Romero and R. H. Davis, "Global Model of Crossflow Microfiltration Based on Hydrodynamic Diffusion," J. Membr. Sci., 39, 157-185 (1988).
14. G. Green and G. Belfort, "Fouling of Ultrafiltration Membranes, Lateral Migration and the Particle Trajectory Model," Desalination, 35, 129-147 (1980).
15. R. K. Warren, D. G. MacDonald, and G. A. Hill, "Cross-Flow Microfiltration of Saccharomyces cerevisiae," Process Biochem., 26(6), 337-342 (1991).
16. G. Taylor, J. A. Levesley, and M. Hoare, "Pilot-Scale Ultrafiltration of Concentrated Protein Precipitate Suspensions: The Effect of Concentration and Fluid Dynamics, Chem. Eng. Commun., 129, 227-250 (1994).
17. G. P. Agarwal, "Analysis of Proteins Transmission in Vortex Flow Ultrafilter for Mass Transfer Coefficient," J. Membr. Sci., 136, 141-151 (1997).
18. K.-H. Kroner and V. Nissinen, "Dynamic Filtration of Microbial Suspensions Using an Annular Rotating Filter," Ibid., 36, 85-100 (1985).
19. R. Datar, "Studies on the Separation of Intracellular Soluble Enzymes from Bacterial Cell Debris by Tangential Flow Membrane Filtration," Biotechnol. Lett., 7(7), 471-476 (1985).
20. M. S. Le, L. B. Spark, and P. S. Ward, "The Separation of Aryl Acrylamidase by Cross Flow Microfiltration and the Significance of Enzyme Cell Debris Interaction," J. Membr. Sci., 21(3), 219-232 (1984).
21. C. Taddei, P. Aimar, G. Daulïn, and V. Sanchez, "Mass Transfer during Sweet Whey Ultrafiltralion on Mineral Membranes, Lait. 66(4), 371-390 (1986).
22. G. Samuelsson, P. Dejmek, G. Tragardh, and M. Paulsson, "Minimising Whey Protein Retention in Cross-Flow Microfiltration of Skim Milk," Int. Dairy J. 7, 237-242 (1997).
23. M. Bennasar, D. Rouleau, R. Mayer, and B. T. de la Fuen, "Ultrafiltration of Milk on Mineral Membranes - Improved Performance," J. Soc. Diary Technol., 35(2), 43-49, (1982).
24. P. Blanpain, J. Hermia, and M. Lenoel, "Mechanisms Governing Permeate Flow and Protein Rejection in the Microfiltration of Beer with a Cyclopore Membrane," J. Membr. Sci., 84(1-2), 37 (1993).
25. M. S. Le and T. Atkinson, "Cross-Flow Microfiltration for Recovery of Intracellular Products," Process Biochem., 20(1), 26-31 (1985).
26. M. Balakrishnan and G. P. Agarwal, "Study of Protein Transmission through Ultrafiltration Membranes," J. Membr. Sci., 85(2), 111 (1993).
27. K. E. Göklen, M. Thien, S. Ayler, S. Smith, E. Fisher, M. Chartrain, P. Salmon, J. Wilson, A. Andrews, and B. Buckland, "Development of Crossflow Filtration Processes for the Commercial Isolation of a Bacterial Lipase," Bioprocess Eng., 11, 49-56 (1994).
28. W. R. Bowen and N. J. Hall, "Properties of Microfiltration Membranes: Mechanisms of Flux Loss in the Recovery of an Enzyme," Biotechnol. Bioeng., 46(1), 28 (1995).
29. J. A. Levesley and M. Hoare, "The Effect of High Frequency Backflushing on the Microfiltration of Yeast Homogenate Suspensions for the Recovery of Soluble Proteins," J. Membr. Sci., 158, 29-39 (1999).