Analysis of two major anti-M2 antibodies (anti-PDC-E2/BCOADC-E2) in primary biliary cirrhosis: Relationship to titers of immunofluorescent anti-mitochondrial antibody

Hepatology Research

Volumn 18, Issue 1, 2000, Pages 1-9

  Miyakawa, Hiroshi ^a   Kitazawa, Eriko ^a   Fujikawa, Hirotoshi ^a   Kikuchi, Kentaro ^a   Abe, Kazuhiro ^a   Kawaguchi, Naomi ^a   Kako, Makoto ^a  

^a TEIKYO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Anti BCOADC E2; Anti PDC E2; Autoimmune cholangitis; Immunoblotting

Indexed keywords

ANTIGEN; HYBRID PROTEIN; MITOCHONDRION ANTIBODY; PYRUVATE DEHYDROGENASE COMPLEX; RECOMBINANT PROTEIN;

ADULT; AGED; ANTIBODY DETECTION; ANTIBODY TITER; ARTICLE; AUTOIMMUNE DISEASE; CATTLE; CHOLANGITIS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HEART MITOCHONDRION; HUMAN; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; MAJOR CLINICAL STUDY; MALE; NONHUMAN; OPTICAL DENSITY; PRIMARY BILIARY CIRRHOSIS; PRIORITY JOURNAL; SEROLOGY;

EID: 0034027257     PISSN: 13866346     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1386-6346(99)00079-0     Document Type: Article

References (26)

1. Mackay I.R. Primary biliary cirrhosis showing a high titer of autoantibody. New Engl. J. Med. 258:1958;185-188.
2. Walker J.G., Doniach D., Roitt I.M., Sherlock S. Serological tests in diagnosis of primary biliary cirrhosis. Lancet. 1:1965;827-831.
3. Invernizzi P., Crosignani A., Battezzati P.M. et al. Comparison of the clinical features and clinical course of antimitochondrial antibody-positive and -negative primary biliary cirrhosis. Hepatology. 25:1997;1090-1095.
4. Kitami N., Komada T., Ishii H. et al. Immunological study of anti-M2 in antimitochondrial antibody-negative primary biliary cirrhosis. Intern. Med. 34:1995;496-501.
5. Lacerda M.A., Ludwig J., Dickson E.R., Jorgensen R.A., Lindor M.D. Antimitochondrial antibody-negative primary biliary cirrhosis. Am. J. Gastroenterol. 90:1990;247-249.
6. Brünner G., Klinge O. A cholangitis with antinuclear antibodies (immuno-cholangitis) resembling chronic non-suppurative destructive cholangitis. Dtsch. Med. Wochenschr. 112:1987;1454-1458.
7. Ben-Ari Z., Dhillon A.P., Sherlock S. Autoimmune cholangiopathy: part of the spectrum of autoimmune chronic active hepatitis. Hepatology. 18:1993;10-15.
8. Gershwin M.E., Mackay I.R., Sturgess A., Coppel R.L. Identification and specificity of a cDNA encoding the 70 KD mitochondrial antigen recognized in primary biliary cirrhosis. J. Immunol. 138:1987;3525-3531.
9. Surh C.D., Coppel R., Gershwin M.E. Structural requirement for autoreactivity on human pyruvate dehydrogenase-E2, the major autoantigen of primary biliary cirrhosis. Implication for a conformational epitope. J. Immunol. 144:1990;3367-3374.
10. Surh C.D., Roche T.E., Danner D.J. et al. Antimitochondrial antibodies in primary biliary cirrhosis recognize cross-reactive epitope(s) on protein X and dihydrolipoamide acetyltransferase of pyruvate dehydrogenese complex. Hepatology. 10:1989;127-133.
11. Lau K.S., Griffin T.A., Hu C.W.C. et al. Conservation of primary structure in the lipoyl-bearing and dihydrolipoyl dehydrogenase binding domains of mammalian branched-chain alpha-keto acid dehydrogenase complex: molecular cloning of human and bovine transacylase (E2) cDNAs. Biochemistry. 27:1988;1972-1981.
12. Surh C.D., Danner D.J., Ahtab A. et al. Reactivity of primary biliary cirrhosis sera with a human fetal liver cDNA clone of branched-chain α-keto acid dehydrogenase dihydrolipoamide acyltransferase, 52 kD mitochondrial autoantigen. Hepatology. 9:1989;63-68.
13. Moteki S., Leung P.S.C., Dickson E.R. et al. Epitope mapping and reactivity of autoantibodies to the E2 component of 2-oxoglutarate dehydrogenase complex in primary biliary cirrhosis using recombinant 2-oxoglutarate dehydrogenase complex. Hepatology. 23:1996;436-444.
14. Fregeau D.R., Roche T.E., Davis P.A., Coppel R., Gershwin M.E. Inhibition of pyruvate dehydrogenase complex activity by autoantibodies specific for E1α, a non-lipoic acid containing mitochondrial enzyme. J. Immunol. 144:1990;1671-1676.
15. Van de Water J., Cooper A., Surh C.D. et al. Detection of autoantibodies to recombinant mitochondrial proteins in patients with primary biliary cirrhosis. New Engl. J. Med. 320:1989;1377-1380.
16. Coppel R.L., McNeilage J., Surh C.D. et al. Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase. Proc. Natl. Acad. Sci. USA. 85:1988;7317-7321.
17. Wiesner R.H., LaRusso N.F., Ludwig J., Dickson E.R. Comparison of the clinicopathologic features in primary sclerosing cholangitis and primary biliary cirrhosis. Gastroenterology. 88:1985;108-114.
18. Scheuer P.J. Primary biliary cirrhosis. Proc. R. Soc. Med. 60:1967;1257-1260.
19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
20. Miyakawa H., Kawaguchi N., Abe K. et al. Serial changes of serum anti-M2 proteins in patients with primary biliary cirrhosis: a follow-up study by immunoblotting. Hepatol. Res. 13:1999;143-152.
21. Miyakawa H., Kawaguchi N., Kikuchi K. et al. Combination assays for IgG class and IgM class anti-pyruvate dehydrogenase complex (PDC)-E2 by ELISA using recombinant autoantigen to diagnose primary biliary cirrhosis. Hepatol. Res. 12:1998;140-150.
22. Berg P.A., Klein R. Heterogeneity of antimitochondrial antibodies. Semin. Liver Dis. 9:1989;103-116.
23. Miyakawa H., Abe K., Kitazawa E. et al. Detection of anti-branched chain 2-oxo acid dehydrogenase complex (BCOADC)-E2 antibody in primary biliary cirrhosis by ELISA using recombinant fusion protein. Autoimmunity. 30:1999;11-20.
24. Kawaguchi N., Miyakawa H., Abe K. et al. Western blot analysis of anti-M2 antibodies in anti-mitochondrial antibody-negative primary biliary cirrhosis. Jpn. J. Clin. Pathol. 45:1997;1079-1084. (in Japanese).
25. Nakanuma Y., Tsuneyama K., Gershwin M.E., Yasoshima M. Pathology and immunopathology of primary biliary cirrhosis with emphasis on bile duct lesions: recent progress. Semin. Liver Dis. 15:1995;313-328.
26. Neuberger J., Thomson R. PBC and AMA - what is the connection? Hepatology. 29:1999;271-276.