Controlled oxidation of calf thymus DNA to produce standard samples for 8-oxo-deoxyguanosine analysis; effects of freeze-drying, storage and hydrolysis conditions

Free Radical Research

Volumn 32, Issue 4, 2000, Pages 327-332

  Wood, Sharon G ^a   Gedik, Catherine M ^a   Collins, Andrew R ^a  

^a ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

8 hydroxy 2' deoxyguanosine; Oxidative DNA damage; Quality control

Indexed keywords

8 HYDROXYDEOXYGUANOSINE; ALKALINE PHOSPHATASE; DEOXYRIBONUCLEASE I; DNA; ISOENZYME; NUCLEASE; PHOSPHODIESTERASE; PHOSPHODIESTERASE I; PHOTOSENSITIZING AGENT;

ARTICLE; DNA DAMAGE; DNA DEGRADATION; DNA DETERMINATION; FREEZE DRYING; HYDROLYSIS; OXIDATION; QUALITY CONTROL; REACTION ANALYSIS; STANDARD; STORAGE;

EID: 0034026742     PISSN: 10715762     EISSN: None     Source Type: Journal    
DOI: 10.1080/10715760000300321     Document Type: Article

References (9)

1. T. Takeuchi, M. Nakajima, Y. Ohta, K. Mure, T. Takeshita and K. Morimoto (1994) Evaluation of 8-hydroxydeoxyguanosine, a typical oxidative DNA damage, in human leukocytes. Carcinogenesis, 15, 1519-1523.
2. T. Hofer and L. Möller (1998) Reduction of oxidation during the preparation of DNA and analysis of 8-hydroxy-2′-deoxyguanosine. Chemical Research in Toxicology, 11, 882-887.
3. E. Kvam and R.M. Tyrrell (1997) Artificial background and induced levels of oxidative base damage in DNA from human cells. Carcinogenesis, 18, 2281-2283.
4. A. Jenner, T.G. England, O.I. Aruoma and B. Halliwell (1998) Measurement of oxidative DNA damage by gas chromatography-mass spectrometry: ethanethiol prevents artifactual generation of oxidized DNA bases. Biochemical Journal, 331, 365-369.
5. H.J. Helbock, K.B. Beckman, M.K. Shigenaga, P.B. Walter, A.A. Woodall, H.C. Yeo and B.N. Ames (1998) DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proceedings of the National Academy of Sciences of the USA, 95, 288-293.
6. C.M. Gedik, S.G. Wood and A.R. Collins (1998) Measuring oxidative damage to DNA; HPLC and the comet assay compared. Free Radical Research, 29, 609-615.
7. M. Pflaum, O. Will, H.-C. Mahler and B. Epe (1998) DNA oxidation products determined with repair endonucleases in mammalian cells: types, basal levels and influence of cell proliferation. Carcinogenesis, 18, 2225-2231.
8. C. Richter, J.-W. Park and B.N. Ames (1988) Normal oxidative damage to mitochondrial and nuclear DNA is extensive. Proceedings of the National Academy of Sciences of the USA, 85, 6465-6467.
9. ESCODD (2000) Comparison of different methods of measuring 8-oxoguamine as a marker of oxidative DNA damage. Free Radical Research, 32, 333-341.