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For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
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Thorson, J.S.1
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Ahlert, J.3
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Onwueme, K.C.5
Ruppen, M.6
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For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
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0030069884
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For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
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For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
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For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
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Nicolaou, K.C.1
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For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
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Nicolaou, K.C.1
Dai, W.-M.2
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0342315827
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When evaluated in p388 and B16 animal models (see ref 3a)
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When evaluated in p388 and B16 animal models (see ref 3a).
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9
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0023820894
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(b) Zein, N.; Sinha, A. M.; McGaharen, W. J.; Ellestad, G. A. Science 1988, 240, 1198-1201.
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(a) Ellestad, G. A.; Ding, W.-D.; Zein, N.; Townsend, C. A. Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995; pp 137-160.
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0033151526
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(a) Sievers, E. L.; Appelbaum, F. R.; Spielberger, R. T.; Forman, S. J.; Flowers, D.; Smith, F. O.; Shannon-Dorcy, K.; Berger, M. S.; Bernstein, I. D. Blood 1999, 93, 3678-3684.
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Bernstein, I.D.9
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19
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0343185253
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note
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-1 ampicillin in E. coli are as follow: cosmids 3a, 4a, 4b, 10a, 13a, and 16a, 0.5; pJT1214 and pJT1232, 5.0; pRE7, 20.0; induced pRE7, 50.0; and pJT1224, pAP6, pRE1, and control plasmids pUC18, pBluescript, and pMAL-C2, <0.01. Given that the cosmid clones which carry calC are known to carry both polyketide synthase and deoxy sugar genes, this work also verifies that we have cloned the 1 biosynthetic gene cluster (Whitwam, R. E.; Ahlert, J.; Shepard, E.; Thorson, J. S., unpublished results).
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20
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0342750640
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note
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8
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21
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0342315817
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note
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9
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22
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0342750639
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note
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-1). The desired mbp-CalC was eluted with buffer A containing 10 mM maltose, concentrated, and resolved on S-300 (50 mM Tris·Cl, pH 7.5, 200 mM NaCl).
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23
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0023899855
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The precise mbp-CalC concentration was determined by quantitative amino acid hydrolysis by the Rockefeller University Protein/DNA Technology Center. Trace metal content of an aliquot of the hydrolysate was subsequently determined by ICP-MS on four distinct mbp-CalC preparations with buffer alone and/or maltose-binding protein alone, analyzed in parallel as controls. Results were independently confirmed by methodology for spectrophotometric Fe determination (Fish, W. W. Methods Enzymol. 1988, 158, 357-364).
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Fish, W.W.1
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24
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0343620774
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note
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Based upon the monomeric molecular weight of 63 576 calculated from the known nucleotide sequence of the mbp-calC gene fusion, which is consistent with the subunit molecular weight determined by SDS-PAGE.
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26
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0342315816
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note
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3-fold excess of 1. Preincubation of mbp-CalC and DTT, protein removal via forced dialysis (Microcon-10), and the subsequent use of the DTT solution as reductant did not noticeably affect the amount of DNA cleavage.
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27
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0342315815
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note
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3+) prior to the activity assay; see ref 15.
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30
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0028933323
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