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Volumn 122, Issue 7, 2000, Pages 1556-1557

The gene calC encodes for a non-heme iron metalloprotein responsible for calicheamicin self-resistance in Micromonospora [9]

Author keywords

[No Author keywords available]

Indexed keywords

CALICHEAMICIN; DOXORUBICIN; METALLOPROTEIN; NONHEME IRON PROTEIN;

EID: 0034000656     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja993612w     Document Type: Letter
Times cited : (25)

References (41)
  • 1
    • 0004221328 scopus 로고    scopus 로고
    • in press
    • For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
    • (2000) Curr. Pharm. Des.
    • Thorson, J.S.1    Sievers, E.L.2    Ahlert, J.3    Shepard, E.4    Onwueme, K.C.5    Ruppen, M.6
  • 2
    • 0032852820 scopus 로고    scopus 로고
    • For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
    • (1999) Bioorg. Chem. , vol.27 , pp. 172-188
    • Thorson, J.S.1    Shen, B.2    Whitwam, R.E.3    Liu, W.4    Li, Y.5    Ahlert, J.6
  • 3
    • 0030069884 scopus 로고    scopus 로고
    • For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
    • (1996) J. Org. Chem. , vol.61 , pp. 16-44
    • Danishefsky, S.J.1    Shair, M.D.2
  • 4
    • 0003815528 scopus 로고
    • Marcel Dekker: New York
    • For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
    • (1995) Enediyne Antibiotics as Antitumor Agents
  • 5
    • 0027319406 scopus 로고
    • For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
    • (1993) Proc. Natl. Acad. Sci. U.S.A. , vol.90 , pp. 5881-5888
    • Nicolaou, K.C.1    Smith, A.L.2    Yue, E.W.3
  • 6
    • 0026045597 scopus 로고
    • For recent reviews see: (a) Thorson, J. S.; Sievers, E. L.; Ahlert, J.; Shepard, E.; Onwueme, K. C.; Ruppen, M. Curr. Pharm. Des. 2000, in press. (b) Thorson, J. S.; Shen, B.; Whitwam, R. E.; Liu, W.; Li, Y.; Ahlert, J. Bioorg. Chem. 1999, 27, 172-188. (c) Danishefsky, S. J.; Shair, M. D. J. Org. Chem. 1996, 61, 16-44. (d) Enediyne Antibiotics as Antitumor Agents; Marcel Dekker: New York, 1995. (e) Nicolaou, K. C.; Smith, A. L.; Yue, E. W. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5881-5888. (f) Nicolaou, K. C.; Dai, W.-M. Angew. Chem., Int. Ed. Engl. 1991, 30, 1387-1416.
    • (1991) Angew. Chem., Int. Ed. Engl. , vol.30 , pp. 1387-1416
    • Nicolaou, K.C.1    Dai, W.-M.2
  • 7
    • 0342315827 scopus 로고    scopus 로고
    • When evaluated in p388 and B16 animal models (see ref 3a)
    • When evaluated in p388 and B16 animal models (see ref 3a).
  • 19
    • 0343185253 scopus 로고    scopus 로고
    • note
    • -1 ampicillin in E. coli are as follow: cosmids 3a, 4a, 4b, 10a, 13a, and 16a, 0.5; pJT1214 and pJT1232, 5.0; pRE7, 20.0; induced pRE7, 50.0; and pJT1224, pAP6, pRE1, and control plasmids pUC18, pBluescript, and pMAL-C2, <0.01. Given that the cosmid clones which carry calC are known to carry both polyketide synthase and deoxy sugar genes, this work also verifies that we have cloned the 1 biosynthetic gene cluster (Whitwam, R. E.; Ahlert, J.; Shepard, E.; Thorson, J. S., unpublished results).
  • 20
    • 0342750640 scopus 로고    scopus 로고
    • note
    • 8
  • 21
    • 0342315817 scopus 로고    scopus 로고
    • note
    • 9
  • 22
    • 0342750639 scopus 로고    scopus 로고
    • note
    • -1). The desired mbp-CalC was eluted with buffer A containing 10 mM maltose, concentrated, and resolved on S-300 (50 mM Tris·Cl, pH 7.5, 200 mM NaCl).
  • 23
    • 0023899855 scopus 로고
    • The precise mbp-CalC concentration was determined by quantitative amino acid hydrolysis by the Rockefeller University Protein/DNA Technology Center. Trace metal content of an aliquot of the hydrolysate was subsequently determined by ICP-MS on four distinct mbp-CalC preparations with buffer alone and/or maltose-binding protein alone, analyzed in parallel as controls. Results were independently confirmed by methodology for spectrophotometric Fe determination (Fish, W. W. Methods Enzymol. 1988, 158, 357-364).
    • (1988) Methods Enzymol. , vol.158 , pp. 357-364
    • Fish, W.W.1
  • 24
    • 0343620774 scopus 로고    scopus 로고
    • note
    • Based upon the monomeric molecular weight of 63 576 calculated from the known nucleotide sequence of the mbp-calC gene fusion, which is consistent with the subunit molecular weight determined by SDS-PAGE.
  • 26
    • 0342315816 scopus 로고    scopus 로고
    • note
    • 3-fold excess of 1. Preincubation of mbp-CalC and DTT, protein removal via forced dialysis (Microcon-10), and the subsequent use of the DTT solution as reductant did not noticeably affect the amount of DNA cleavage.
  • 27
    • 0342315815 scopus 로고    scopus 로고
    • note
    • 3+) prior to the activity assay; see ref 15.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.