PIG-B: A homemade monophasic cocktail for the extraction of RNA

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 9, Issue 1, 1998, Pages 73-77

  Weber, Kristy ^a   Bolander, Mark E ^a   Sarkar, Gobinda ^a  

^a MAYO CLINIC   (United States)

Author keywords

Reagent; RNA extraction; RT PCR amplification

Indexed keywords

GUANIDINE; ISOPENTYL ALCOHOL; ISOTHIOCYANIC ACID; MERCAPTOETHANOL; PHENOL; REAGENT; RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARTILAGE CELL; HEART; HUMAN; HUMAN TISSUE; LIVER; NONHUMAN; POLYMERASE CHAIN REACTION; RABBIT; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SKELETAL MUSCLE;

EID: 0031867449     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02752699     Document Type: Article

References (6)

1. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
3. Chomczynski, P. (1993) A reagent for the single-step simultaneous isolation of RNA, DNA, and proteins from cell and tissue samples. Biotechniques 15, 532-536.
4. Sarkar, G. and Bolander, M. E. (1994) The "Looped Oligo" method for generating reference molecules for quantitative PCR. Biotechniques 17, 864-866.
5. Sarkar, G. and Sommer, S. S. (1989) Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity. Science 244, 331-334.
6. Kirby, K. S. (1956) A new method for the isolation of nucleic acids from mammalian tissues. Biochem. J. 64, 405-411.