Anaerobic toluene catabolism of Thauera aromatica: The bbs operon codes for enzymes of β oxidation of the intermediate benzylsuccinate

Journal of Bacteriology

Volumn 182, Issue 2, 2000, Pages 272-277

  Leuthner, Birgitta ^a   Heider, Johann ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

3 HYDROXYACYL COENZYME A DEHYDROGENASE; ACETYL COENZYME A ACYLTRANSFERASE; BACTERIAL DNA; BENZOYL COENZYME A; BENZYLSUCCINATE SYNTHASE; COENZYME A; ENOYL COENZYME A HYDRATASE; MESSENGER RNA; SYNTHETASE; TOLUENE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANAEROBIC BACTERIUM; ARTICLE; CATABOLISM; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GENE MAPPING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; OXIDATION; PRIORITY JOURNAL; PROMOTER REGION; THAUERA AROMATICA; TRANSCRIPTION REGULATION;

ACETYL-COA C-ACYLTRANSFERASE; AMINO ACID SEQUENCE; ANAEROBIOSIS; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; ENOYL-COA HYDRATASE; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; OPERON; OXIDOREDUCTASES; SUCCINATES; THAUERA; TOLUENE;

EID: 0033989161     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.2.272-277.2000     Document Type: Article

References (31)

1. Aiba, H., S. Adhya, and B. deCombrugge. 1981. Evidence for two functional gal promoters in intact Escherichia coli. J. Biol. Chem. 256:11905-11910.
2. Anders, A., A. Kaetzke, P. Kämpfer, W. Ludwig, and G. Fuchs. 1995. Taxonomic position of aromatic degrading denitrifying pseudomonad strains K172 and KB740 and their description as new members of the genera Thauera, T. aromatica sp. nov., and Azoarcus, A. evansii sp. nov., respectively, members of the beta subclass of Proteobacteria. Int. J. Syst. Bacteriol. 45: 327-331
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1987. Current protocols in molecular biology. John Wiley & Sons, New York, N.Y.
4. Beller, H. R., and A. M. Spormann. 1997. Anaerobic activation of toluene and o-xylene by addition to fumarate in denitrifying strain T. J. Bacteriol. 179:670-676.
5. Biegert, T., G. Fuchs, and J. Heider. 1996. Evidence that oxidation of toluene in the denitrifying bacterium Thauera aromatica is initiated by formation of henzylsuccinate from toluene and fumarate. Eur. J. Biochem. 238:661-668.
6. Boorstein, W. R., and E. A. Craig. 1989. Primer extension analysis from RNA. Methods Enzymol. 180:347-369.
7. Breese, K., and G. Fuchs. 1998. 4-Hydroxybenzoyl-CoA reductase (dehydroxylating) from the denitrifying bacterium Thauera aromatica. Prosthetic groups, electron donor, and genes of a member of the molybdenum-flavin-iron-sulfur proteins. Eur. J. Biochem. 251:916-923.
8. Chung, C. T., S. L. Niemela, and R. H. Miller. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86:2172-2175.
9. Giese, B., and J. Meister. 1977. Die Addition von Kohlenwasserstoffen an Olefine: eine neue synthetische Methode. Chem. Ber. 110:2588-2600.
10. Heider, J., M. Boll, K. Breese, S. Breinig, C. Ebenau-Jehle, U. Feil, N. Gad'on, D. Laempe, B. Leuthner, M. Mohamed, S. Schneider, G. Burchhardt, and G. Fuchs. 1998. Differential induction of enzymes involved in anaerobic metabolism of aromatic compounds in the denitrifying bacterium Thauera aromatica. Arch. Microbiol. 170:120-131.
11. Heider, J., and G. Fuchs. 1997. Anaerobic metabolism of aromatic compounds. Eur. J. Biochem. 243:577-596.
12. Heider, J., and G. Fuchs. 1997. Microbial anaerobic aromatic metabolism. Anaerobe 3:1-22.
13. Heider, J., A. M. Spormann, H. R. Beller, and F. Widdel. 1999. Anaerobic bacterial metabolism of hydrocarbons. FEMS Microbiol. Rev. 22:459-473.
14. Hofstein, H. A., Y. Feng, V. E. Anderson, and P. J. Tonge. 1999. Role of glutamate 144 and glutamate 164 in the catalytic mechanism of enoyl-CoA hydratase. Biochemistry 38:9508-9516.
15. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
16. Kim, J.-J. P., M. Wang, and R. Paschke. 1993. Crystal structures of medium-chain acyl-CoA dehydrogenase from pig liver mitrochondria with and without substrate. Proc. Natl. Acad. Sci. USA 90:7523-7527.
17. Kuhn, E. P., P. J. Colberg, J. L. Schnoor, O. Wanner, A. J. B. Zehnder, and R. P. Schwarzenbach. 1985. Microbial transformations of substituted benzenes during infiltration of river water to groundwater: laboratory column studies. Environ. Sci. Technol. 19:961-968.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
19. Lehman, T. C., D. E. Hale, A. Bhala, and C. Thorpe. 1990. An acyl-coenzyme A dehydrogenase assay using ferricenium ion. Anal. Biochem. 186:280-284.
20. Leuthner, B., C. Leutwein, H. Schultz, P. Hörth, W. Haehnel, E. Schiltz, H. Schägger, and J. Heider. 1998. Biochemical and genetic characterisation of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism. Mol. Microbiol. 28:615-628.
21. Leuthner, B., and J. Heider. 1998. A two-component system involved in regulation of anaerobic toluene metabolism in Thauera aromatica. FEMS Microbiol. Lett. 166:35-41.
22. Leutwein, C., and J. Heider. 1999. Anaerobic toluene catabolic pathway in denitrifying Thauera aromatica: activation and β-oxidation of the first intermediate. (R)-(+)-benzylsuccinate. Microbiology 145:3265-3271.
23. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
24. O'Farrell, P. H. 1975. High resolution two-dimensional electrophoresis of protein. J. Biol. Chem. 250:4007-4021.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Seedorf, U., M. Raabe, P. Ellinghaus, F. Kannenberg, M. Fobker, T. Engel, S. Denis, F. Wouters, K. W. A. Wirtz, R. J. A. Wanders, N. Meada, and G. Assmann. 1998. Defective peroxisomal catabolism of branched fatty acyl coenzyme A in mice lacking the sterol carrier protein-x gene function. Genes Dev. 12:1189-1201.
27. Sidhu, H., S. D. Ogden, H.-Y. Lung, B. G. Luttge, A. L. Baetz, and A. B. Peck. 1997. DNA sequencing and expression of the formyl-coenzyme A transferase gene, frc, from Oxalobacter formigenes. J. Bacteriol. 179:3378-3381.
28. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 polymerase/ promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078.
29. Tschech, A., and G. Fuchs. 1987. Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads. Arch. Microbiol. 148: 213-217.
30. Vogel, T. M., and D. Grbic-Galic. 1986. Incorporation of oxygen from water into toluene and benzene during anaerobic fermentative transformation. Appl. Environ. Microbiol. 52:200-202.
31. Wilson, G. H., G. B. Smith, and J. H. Rees. 1986. Biotransformations of selected alkylbenzenes and halogenated aliphatic hydrocarbons in methanogenic aquifer material: a microcosm study. Environ. Sci. Technol. 20:997-1002.