Evidence that anaerobic oxidation of toluene in the denitrifying bacterium Thauera aromatica is initiated by formation of benzylsuccinate from toluene and fumarate

European Journal of Biochemistry

Volumn 238, Issue 3, 1996, Pages 661-668

  Biegert, Thomas ^b   Fuchs, Georg ^b   Heider, Johann ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b NONE

Author keywords

anaerobic; benzylsuccinate; benzylsuccinate synthase; degradation; toluene

Indexed keywords

FUMARIC ACID; SUCCINIC ACID DERIVATIVE; TOLUENE;

ANAEROBIC METABOLISM; ARTICLE; BACTERIAL METABOLISM; BIODEGRADATION; CONTROLLED STUDY; DENITRIFICATION; NONHUMAN; POLLUTANT; PRIORITY JOURNAL; REACTION ANALYSIS;

BACTERIA (MICROORGANISMS); THAUERA AROMATICA;

EID: 0030036363     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0661w.x     Document Type: Article

References (30)

1. 2 by a new type of sulfate-reducing bacteria, Arch. Microbiol. 156, 5-14.
2. Altenschmidt, U. & Fuchs, G. (1991) Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methylhydroxylation and benzoyl-CoA as central aromatic intermediate, Arch. Microbiol. 156, 152-158.
3. Altenschmidt, U. & Fuchs, G. (1992) Anaerobic toluene oxidation to benzyl alcohol and benzaldehyde in a denitrifying Pseudomonas strain, J. Bacteriol. 174, 4860-4862.
4. Anders, A., Kaetzke, A., Kämpfer, P., Ludwig, W. & Fuchs, G. (1995) Taxonomic position of aromatic degrading denitrifying pseudomonad strains K172 and KB740. Description of a new member of the genus Thauera, Thauera aromatica sp. nov. and of the genus Azoarcus, Azoarcus evansii sp. nov., members of the β subclass of proteobacteria, Int. J. Syst. Bacteriol. 45, 327-333.
5. Beller, H. R., Grbic-Galic, D. & Reinhard, M. (1992) Microbial degradation of toluene under sulfate-reducing conditions and the influence of iron on the process, Appl. Env. Microbiol. 58, 786-793.
6. Biegert, T. & Fuchs, G. (1995) Anaerobic oxidation of toluene (analogues) to benzoate (analogues) by whole cells and by cell extracts of a denitrifying Thauera sp., Arch. Microbiol. 163, 407-417.
7. Biegert, T., Altenschmidt, U., Eckerskorn, C. & Fuchs, G. (1995) Purification and properties of benzyl alcohol dehydrogenase from a denitrifying Thauera sp., Arch. Microbiol. 163, 418-423.
8. Boll, M. & Fuchs, G. (1995) Benzoyl-CoA reductase (dearomatizing). a key enzyme of anaerobic aromatic metabolism: ATP dependence of the reaction, purification and some properties of the enzyme from Thauera aromatica strain K172, Eur. J. Biochem. 234, 921-934.
9. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
10. Dagley, S. (1984) Microbial degradation of aromatic compounds, Dev. Int. Microbiol. 25, 53-65.
11. Dolfing, J., Zeyer, J., Binder-Eicher, P. & Schwarzenbach, R. P. (1990) Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen, Arch. Microbiol. 155, 256-262.
12. Evans, P. J., Mang, D. T., Kim, K. S. & Youn,. L. Y. (1991) Anaerobic degradation of toluene by a denitrifying bacterium, Appl. Env. Microbiol. 57, 1139-1145.
13. Evans, P. J., Ling, W., Goldschmidt, B., Ritter, E. R. & Young, L. Y. (1992) Metabolites formed during anaerobic transformations of toluene and o-xylene and their proposed relationship to the initial steps of toluene mineralization, Appl. Env. Microbiol. 58, 496-501.
14. Fries, M. R., Zhou, J., Chee-Sanford, J. & Tiedje, J. M. (1994) Isolation, characterization and distribution of denitrifying toluene-degraders from a variety of habitats, Appl. Evn. Microbiol. 60, 2801-2810.
15. Gibson, D. T. & Subramanian, V. (1984) Microbial degradation of organic compounds pp. 181-252, Dekker, New York.
16. Koch, J. & Fuchs, G. (1992) Enzymatic reduction of benzoyl-CoA to alicyclic compounds, a key reaction in anaerobic aromatic metabolism, Eur. J. Biochem. 205, 195-202.
17. Lochmeyer, C. & Fuchs, G. (1990) NADP -specific 2-oxoglutarate dehydrogenase in denitrifying Pseudomonas species, Arch. Microbiol. 153, 226-229.
18. Lovley, D. R. & Lonergan, D. J. (1990) Anaerobic oxidation of toluene, phenol and p-cresol by the dissimilatory iron-reducing organism GS-15, Appl. Env. Microbiol. 56, 1858-1864.
19. Metzler, D. E. (1977) Biochemistry, Academic Press, New York.
20. Pine, S. H. (1987) Organic chemistry, McGraw-Hill, New York.
21. Rabus, R., Nordhaus, R., Ludwig, W. & Widdel, F. (1993) Complete oxidation of toluene under strictly anaerobic conditions by a new sulfate-reducing bacterium, Appl. Env. Microbiol. 59, 1444-1451.
22. Rabus, R. & Widdel, F. (1995a) Anaerobic degradation of ethylbenzene and other aromatic hydrocarbons by new denitrifying bacteria, Arch. Microbiol. 163, 96-103.
23. Rabus, R. & Widdle, F. (1995b) Conversion studies with substrate analogues of toluene in a sulfate-reducing bacterium, strain To12, Arch. Microbiol. 164, 448-451.
24. Schocher, R. J., Seyfried, B., Vazquez, F. & Zeyer, J. (1991) Anaerobic degradation of toluene by pure cultures of denitrifying bacteria, Arch. Microbiol. 157, 7-12.
25. Seyfried, B., Glod, G., Schocher, R., Tschech, A. & Zeyer, J. (1994) Initial reactions in the anaerobic oxidation of toluene and m-xylene by denitrifying bacteria, Appl. Env. Microbiol. 157, 7-12.
26. Tschech, A. & Fuchs, G. (1987) Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads, Arch. Microbiol. 148, 213-217.
27. Vogel, T. M. & Grbic-Galic, D. (1986) Incorporation of oxygen from water into toluene and benzene during anaerobic fermentative transformation, Appl. Env. Microbiol. 52, 200-202.
28. Wilson, B. H., Smith, G. B. & Rees, J. H. (1986) Biotransformations of selected alkylbenzenes and halogenated aliphatic hydrocarbons in methanogenic aquifer material: a microcosm study, Environ. Sci. Technol. 20, 997-1002.
29. Zeyer, J., Kuhn, E. P. & Schwarzenbach, R. P. (1986) Rapid microbial mineralization of toluene and 1,3-dimethylbenzene in the absence of molecular oxygen, Appl. Env. Microbiol. 52, 944-947.
30. Zollner, H. (1993) Handbook of enzyme inhibitors, VCH, Weinheim.