The efficacy of laboratory diagnosis of Helicobacter pylori infections in gastric biopsy specimens is related to bacterial density and vacA, cagA, and iceA genotypes

Journal of Clinical Microbiology

Volumn 38, Issue 1, 2000, Pages 13-17

  Van Doorn, Leen Jan ^a   Henskens, Yvonne ^b   Nouhan, Nathalie ^a   Verschuuren, Anita ^a   Vreede, Rolf ^b   Herbink, Paul ^b   Ponjee, Gabriëlle ^b   Van Krimpen, Kees ^b   Blankenburg, Ruud ^c   Scherpenisse, Joost ^c   Quint, Wim ^a  

^a Delft Diagnostic Laboratory   (Netherlands)

^b Department of Clinical Chemistry ^*  (Netherlands)

^c REINIER DE GRAAF HOSPITAL   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; GASTRITIS; GRAM NEGATIVE INFECTION; HELICOBACTER PYLORI; HUMAN; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STOMACH BIOPSY;

EID: 0033988127     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.1.13-17.2000     Document Type: Article

References (27)

1. Akopyants, N. S., S. W. Clifton, D. Kersulyte, J. E. Crabtree, B. E. Youree, C. A. Reece, N. O. Bukanov, E. S. Drazek, B. A. Roe, and D. E. Berg. 1998. Analyses of the cag pathogenicity island of Helicobacter pylori. Mol. Microbiol. 28:37-54.
2. Atherton, J. C. 1997. The clinical relevance of strain types of Helicobacter pylori. Gut 40:701-703.
3. Atherton, J. C., P. Cao, R. M. J. Peek, M. K. Tummuru, M. J. Blaser, and T. L. Cover. 1995. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. Association of specific vacA types with cytotoxin production and peptic ulceration. J. Biol. Chem. 270:17771-17777.
4. Atherton, J. C., R. M. J. Peek, K. T. Tham, T. L. Cover, and M. J. Blaser. 1997. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 112:92-99.
5. Blaser, M. J. 1995. Intrastrain differences in Helicobacter pylori: a key question in mucosal damage? Ann. Med. 27:559-563.
6. Blaser, M. J. 1997. Ecology of Helicobacter pylori in the human stomach. J. Clin. Investig. 100:759-762.
7. Boom, R., C. J. Sol, M. M. Salimans, C. L. Jansen, P. M. Wertheim-van Dillen, and J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
8. Censini, S., C. Lange, Z. Xiang, J. E. Crabtree, P. Ghiara, M. Borodovsky, R. Rappuoli, and A. Covacci. 1996. cag. a pathogenicity island of Helicobacter pylori, encodes type 1-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA 93:14648-14653.
9. Cover, T. L. 1996. The vacuolating cytotoxin of Helicobacter pylori. Mol. Microbiol. 20:241-246.
10. Cover, T. L., D. E. Berg, and M. J. Blaser. 1997. vacA and the cag pathogenicity island of H. pylori, p. 75-90. In P. B. Ernst, P. Michetti, and P. D. Smith (ed.), The immunobiology of Helicobacter pylori. Lippencott-Raven Publishers, Philadelphia, Pa.
11. Cover, T. L., and M. J. Blaser. 1912. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 267:10570-10575.
12. Cover, T. L., M. K. Tummuru, P. Cao, S. A. Thompson, and M. J. Blaser. 1994. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 269:10566-10573.
13. Labigne, A., and H. de Reuse. 1996. Determinants of Helicobacter pylori pathogenicity. Infect. Agents Dis. 5:191-202.
14. Leunk, R. D., P. T. Johnson, B. S. David, W. G. Kraft, and D. R. Morgan. 1988. Cytotoxic activity in broth culture filtrates of Campylobacter pylori. J. Med. Microbiol. 26:93-99.
15. Mobley, H. L. 1997. Defining Helicobacter pylori as a pathogen: strain heterogeneity and virulence. Am, J. Med. 100:2S-11S.
16. Moshkowitz, M., F. M. Konikoff, Y. Peled, M. Santo, A. Hallak, Y. Bujanover, E. Tiomny, and T. Gilat. 1995. High Helicobacter pylori numbers are associated with low eradication rate after triple therapy. Gut 36:845-847.
17. Peek, R. M. J., S. A. Thompson, J. P. Donahue, K. T. Tham, J. C. Atherton, M. J. Blaser, and G. G. Miller. 1998. Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome. Proc. Am. Assoc. Phys. 110:531-544.
18. Phadnis, S. H., D. Ilver, L. Janzon, S. Normark, and T. U. Westblom. 1994. Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infect. Immun. 62:1557-1565.
19. Schmitt, W., and R. Haas. 1994. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin: structural similarities with the IgA protease type of exported protein. Mol. Microbiol. 12:307-319.
20. Telford, J. L., P. Ghiara, M. Dell'Orco, M. Comanducci, D. Burroni, M. Bugnoli, M. F. Tecce, S. Censini, A. Covacci, and Z. Xiang. 1994. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med. 179:1653-1658.
21. Tummuru, M. K., S. A. Sharma, and M. J. Blaser. 1995. Helicobacter pylori picB, a homologue of the Bordetella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells. Mol. Microbiol. 18:867-876.
22. van Doorn, L. J., C. Figueiredo, F. Mégraud, A. S. Pena, P. Midolo, D. M. Queiroz, F. Carneiro, B. Vandenborght, M. G. F. Pégado, R. Sanna, W. A. de Boer, P. Schneeberger, P. Correa, E. K. Ng, J. C. Atherton, M. J. Blaser, and W. G. V. Quint. 1999. Geographic distribution of vacA allelic types of Helicobacter pylori. Gastroenterology 116:823-830.
23. van Doorn, L. J., C. Figueiredo, R. Rossau, G. Jannes, M. van Asbroeck, J. C. Sousa, F. Carneiro, and W. Quint. 1998. Typing of the Helicobacter pylori vacA gene and detection of the cagA gene by PCR and reverse hybridization. J. Clin. Microbiol. 36:1271-1276.
24. van Doorn, L. J., C. Figueiredo, R. Sanna, A. S. Pena, P. Midolo, E. K. Ng, J. C. Atherton, M. J. Blaser, and W. Quint. 1998. Expanding allelic diversity of Helicobacter pylori vacA. J. Clin. Microbiol. 36:2597-2603.
25. van Doorn, L. J., C. Figueiredo, R. Sanna, A. Plaisier, P. Schneeberger, W. A. de Boer, and W. Quint. 1998. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology 115:58-66.
26. van Doorn, L. J., W. G. V. Quint, P. M. Schneeberger, G. N. J. Tytgat, and W. A. de Boer. 1997. Association between vacA and cagA status of Helicobacter pylori and the efficacy of a 1-day quadruple therapy. Lancet 350:71-72.
27. van Doorn, L. J., P. Schneeberger, N. Nouhan, A. P. Plaiser, W. G. V. Quint, and W. A. de Boer. Gut, in press.