Biolistic transformation of macro- and micronuclei

Methods in Cell Biology

Volumn , Issue 62, 2000, Pages 501-512

  Bruns, Peter J ^a   Cassidy Hanley, Donna ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; CELL NUCLEUS; GENETIC TRANSFORMATION; GERMLINE MICRONUCLEUS; REVIEW; TETRAHYMENA THERMOPHILA;

ANIMALS; CELL NUCLEUS; MICRONUCLEUS, GERMLINE; TETRAHYMENA THERMOPHILA; TRANSFORMATION, GENETIC;

EID: 0033986963     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (13)

1. Brunk, C. F., and Navas, P. (1988). Transformation of Tetrahymena thermophila by electroporation and parameters effecting cell survival. Exp. Cell Res. 174(2), 525-532.
2. Cassidy-Hanley, D., Bowen, J., Lee, J. H., Cole, E., VerPlank, L. A., Gaertig, J., Gorovsky, M. A., and Bruns, P. J. (1997). Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics 146(1), 135-147.
3. Cassidy-Hanley, D., Sampath, S. C., and Bruns, P. J. (1999). In preparation.
4. Gaertig, J., and Gorovsky, M. A. (1992). Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants. Proc. Natl. Acad. Sci. U.S.A. 89(19), 9196-9200.
5. Gorovsky, M. A. (1980). Genome organization and reorganization in Tetrahymena. Annu. Rev. Genet. 14, 203-239.
6. Gorovsky, M. A., Yao, M. C., Keevert, J. B., and Pleger, G. L. (1975). Isolation of micro- and macronuclei of Tetrahymena pyriformis. Methods Cell Biol 9(0), 311-327.
7. Hai, B., and Gorovsky, M. A. (1997). Germ-line knockout heterokaryons of an essential alpha-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germ-line nuclei in Tetrahymena thermophila. Proc. Natl. Acad. Sci. U.S.A. 94(4), 1310-1315.
8. Heiser, W. (1998). Optimization of biolistic transformation by the helium-driven PDS-1000/He system. BioRad Online Library and Literature, available at: http://www.bio-rad.com/45811.html
9. Kahn, R. W., Andersen, B. H., and Brunk, C. F. (1993). Transformation of Tetrahymena thermophila by microinjection of a foreign gene. Proc. Natl. Acad. Sci. U.S.A. 90(20), 9295-9299.
10. Orias, E., Larson, D., Hu, Y. F., Yu, G. L., Karttunen, J., Lovlie, A., Haller, B. and Blackburn, E. H. (1988). Replacement of the macronuclear ribosomal RNA genes of a mutant Tetrahymena using electroporation. Gene 70(2), 295-301.
11. Sanford, J. C., Devit, M. J., Russell, J. A., Smith, F. D., Harpending, P. R., Roy, M. K., and Johnston, S. A. (1991). An improved helium-driven biolistic device. Technique 3(1), 3-16.
12. Tondravi, M. M., and Yao, M. C. (1986). Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes. Proc. Natl. Acad. Sci. U.S.A. 83(12), 4369-4373.
13. Yao, M. C., and Yao, C. H. (1991). Transformation of Tetrahymena to cycloheximide resistance with a ribosomal protein gene through sequence replacement. Proc. Natl. Acad. Sci. U.S.A. 88(21), 9493-9497.