Isolation and characterization of in vivo modified histones and an activity gel assay for identification of histone acetyltransferases

Methods in Cell Biology

Volumn , Issue 62, 2000, Pages 379-394

  Wiley, Emily A ^a   Mizzen, Craig A ^b   Allis, C David ^b  

^a Fred Hutchinson Cancer Research Center   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; HISTONE; HISTONE ACETYLTRANSFERASE; SACCHAROMYCES CEREVISIAE PROTEIN;

ANIMAL; CHEMISTRY; ENZYMOLOGY; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVIEW; TETRAHYMENA THERMOPHILA;

ACETYLTRANSFERASES; ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTONE ACETYLTRANSFERASES; HISTONES; SACCHAROMYCES CEREVISIAE PROTEINS; TETRAHYMENA THERMOPHILA;

EID: 0033986794     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (36)

1. Allis, C. D., and Dennison, D. K. (1982). Identification and purification of young macronuclear anlagen from conjugating cells of Tetrahymena thermophila. Dev. Biol. 93, 519-533.
2. Allis, C. D., Glover, C. V., and Gorovsky, M. A. (1979). Micronuclei of Tetrahymena contain two types of histone H3. Proc. Natl. Acad. Sci. U.S.A. 76, 4857-4861.
3. Allis, C. D., Glover, C. V., Bowen, J. K., and Gorovsky, M. A. (1980). Histone variants specific to the transcriptionally active, amitotically dividing macronucleus of the unicellular eucaryote, Tetrahymena thermophila. Cell (Cambridge, Mass.) 20, 609-617.
4. Allis, C. D., Chicoine, L. G., Richman, R., and Schulman, I. G. (1985). Deposition-related histone acetylation in micronuclei of conjugating Tetraymena. Proc. Natl. Acad. Sci. U.S.A. 82, 8048-8052.
5. Allis, C. D., Richman, R., Gorovsky, M. A., Ziegler, Y. S., Touchstone, B., Bradley, W. A., and Cook, R. G. (1986). hv1 is an evolutionarily conserved H2A variant that is preferentially associated with active genes. J. Biol. Chem. 261, 1941-1948.
6. Braunstein, M., Sobel, R. E., Allis, C. D., Turner, B. M., and Broach, J. R. (1996). Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern. Mol. Cell. Biol. 16, 4349-4356.
7. Brownell, J. E., and Allis, C. D. (1995). An activity gel assay detects a single, catalytically active histone acetyltransferase subunit in Tetrahymena macronuclei. Proc. Natl. Acad. Sci. U.S.A. 92, 6364-6368.
8. Brownell, J. E., and Allis, C. D. (1996). Special HATs for special occasions: Linking histone acetylation to chromatin assembly and gene activation. Curr. Opin. Genet. Dev. 6(2), 176-184.
9. Brownell, J. E., Zhou, J., Ranalli, T. A., Kobayashi, R., Roth, S. Y., and Allis, C. D. (1996). Tetrahymena histone acetyltransferase A: A homolog to yeast GCN5 linking histone acetylation to gene activation. Cell (Cambridge, Mass.) 84, 843-851.
10. Brownell, J. E., Mizzen C. A., and Allis, C. D. (1998). An SDS-PAGE based enzyme activity assay for the detection and identification of histone acetyltransferases. In "Methods in Enzymology" (in press).
11. Bruns, P. J., and Brussard, T. B. (1974). Pair formation in Tetrahymena pyriformis, an inducible developmental system. J. Exp. Zool. 188, 337-344.
12. Chicoine, L. G., Schulman, I. G., Richman, R., Cook, R. G., and Allis, C. D. (1986). Nonrandom utilization of acetylation sites in histones isolated from Tetrahymena. J. Biol. Chem. 261, 1071-1076.
13. Felsenfeld, G. (1996). Chromatin unfolds. Cell (Cambridge, Mass.) 86, 13-19.
14. Fusauchi, Y., and Iwai, K. (1983). Tetrahymena histone H2A. Isolation and two variant sequences. J. Biochem. (Tokyo) 95, 147-154.
15. Glover, C. V. C., Vavra, K., Guttman, S. D., and Gorovsky, M. A. (1981). Heat shock and deciliation induce phosphorylation of histone H1 in T. pyriformis. Cell (Cambridge, Mass.) 23, 73-77.
16. Gorovsky, M. A., Yao, M.-C., Keevert, J. B., and Pleger, G. L. (1975). Isolation of micro- and macronuclei of Tetrahymena pyriformis. Methods Cell Biol. 9, 311-327.
17. Guttman, S. D., Glover, C. V., Allis, C. D., and Gorovsky, M. A. (1980). Heat shock, deciliation and release from anoxia induce the synthesis of the same set of polypeptides in starved T. pyriformis. Cell (Cambridge, Mass.) 22, 299-307.
18. Hendzel, M. J., Wei, Y., Mancini, M. A., Van Hooser, A., Ranalli, T., Brinkley, B. R., Bazett-Jones, D. P., and Allis, C. D. (1997). Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation. Chromosoma 106, 348-360.
19. Hutchcroft, J. E., Anostario, M., Harrison, M. L., and Geahlen, R. L. (1991). Renaturation and assay of protein kinases after electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. In "Methods in Enzymology" (T. Hunter and B. M. Sefton, eds.), Vol. 200, pp. 417-423. Academic Press, San Diego, CA.
20. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
21. Levy-Wilson B., Denker, M. S., and Ito, E. (1983). Isolation, characterization, and postsynthestic modifications of Tetrahymena high mobility group proteins. Biochemistry 22, 1715-1721.
22. Lin, R., Leone, J. W., Cook, R. G., and Allis, C. D. (1989). Antibodies specific to acetylated histones document the existence of deposition- and transcription-related histone acetylation in Tetrahymena. J. Cell Biol. 108, 1577-1588.
23. Lu, M. J., Dadd, C. A., Mizzen, C. A., Perry, C. A., McLauchlan, D. R., Annunziato, A. T., and Allis, C. D. (1994). Generation and characterization of novel antibodies highly selective for phosphorylated linker histone H1 in Tetrahymena and HeLa cells. Chromosoma 103, 111-121.
24. Martindale, D. W., Allis, C. D., and Bruns, P. J. (1982). Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages. Exp. Cell Res. 140, 227-236.
25. Mizzen, C. A., and Allis, C. D. (1998). Linking histone acetylation to transcriptional regulation. Cell. Mol. Life Sci. 54(1), 6-20.
26. II250 subunit of TFIID has histone acetyltransferase activity. Cell (Cambridge, Mass.) 87, 1261-1270.
27. Mizzen, C. A., Dou, Y., Liu, Y., Allis, C. D., and Gorovsky, M. (1999). Identification and mutation of phosphorylation sites in a linker histone. Phosphorylation of macronuclear hi is not essential for viability in Tetrahymena. J. Biol. Chem. 274(21), 14533-14536.
28. Ohba, R., Steger, D. J., Brownell, J. E., Mizzen, C. A., Cook, R. J., Cole, J., Workman, J. L., and Allis, C. D. (1999). A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila. Mol. Cell Biol. 19(3), 2061-2068.
29. Pazin, M. J., and Kadanoga, J. T. (1997). What's up and down with histone deacetylation and transcription? Cell (Cambridge, Mass.) 89, 325-328.
30. Roth, S. Y., Shulman, I. G., Richman, R., Cook, R. G., and Allis, C. D. (1988). Characterization of phosphorylation sites in histone H1 in the amitotic macronucleus of Tetrahymena during different physiological states. J. Cell Biol. 107(6), 2473-2482.
31. Roth, S. Y., Collini, M. P., Draetta, G., Beach, D., and Allis, C. D. (1991). A cdc2-like kinase phosphorylates histone H1 in the amitotic macronucleus of Tetrahymena. EMBO J. 10, 2069-2075.
32. Schulman, I. G., Wang, T., Wu, M., Bowen, J., Cook, R. G., Gorovsky, M. A., and Allis, C. D. (1991). Macronuclei and micronuclei in Tetrahymena thermophila contain high-mobility-group-like chromosomal proteins containing a highly conserved eleven-amino-acid putative DNA-binding sequence. Mol. Cell. Biol. 11, 166-174.
33. Sweet, M. T., and Allis, C. D. (1998). Culture and manipulation of Tetrahymena. In "Cells: Culture and Biochemical Analysis of Cells" (D. L. Spector, R. D. Goldman, and L. A. Leinwand, eds.), Vol. 1. Cold Spring Harbor Laboratory Press.
34. Sweet, M. T., Jones, K., and Allis, C. D. (1996). Phosphorylation of linker histone is associated with transcriptional activation in a normally silent nucleus. J. Cell Biol. 135, 1219-1228.
35. Wang, T., and Allis, C. D. (1993). An abundant high-mobility-group-like protein is targeted to micronuclei in a cell cycle-dependent and developmentally regulated fashion in Tetrahymena thermophila. Mol. Cell. Biol. 13, 163-173.
36. Wiley, E. A., Yao, M.-C., and Allis, C. D. (1999). To be published.