메뉴 건너뛰기




Volumn 39, Issue 1, 2000, Pages 36-44

Analysis of enzyme kinetics in individual living cells utilizing fluorescence intensity and polarization measurements

Author keywords

Cellscan; Fluorescence polarization; Intracellular enzyme kinetics

Indexed keywords

CELL ENZYME; FLUORESCEIN DIACETATE;

EID: 0033986636     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(20000101)39:1<36::AID-CYTO6>3.0.CO;2-6     Document Type: Article
Times cited : (21)

References (29)
  • 1
    • 0024021765 scopus 로고
    • Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases
    • Dive C, Cox H, Watson JV, Workman P. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol Cell Probes 1988;2(2):131-145.
    • (1988) Mol Cell Probes , vol.2 , Issue.2 , pp. 131-145
    • Dive, C.1    Cox, H.2    Watson, J.V.3    Workman, P.4
  • 2
    • 0023605884 scopus 로고
    • Improved methodology for intracellular enzyme reaction and inhibition kinetics by flow cytometry
    • Dive C, Workman P, Watson JV. Improved methodology for intracellular enzyme reaction and inhibition kinetics by flow cytometry. Cytometry 1987;8:552-561.
    • (1987) Cytometry , vol.8 , pp. 552-561
    • Dive, C.1    Workman, P.2    Watson, J.V.3
  • 3
    • 0023716224 scopus 로고
    • Inhibition of intracellular esterases by antitumour chloroethylnitrosoureas. Measurement by flow cytometry and correlation with molecular carbamoylation activity
    • Dive C, Workman P, Watson JV. Inhibition of intracellular esterases by antitumour chloroethylnitrosoureas. Measurement by flow cytometry and correlation with molecular carbamoylation activity. Biochem Pharmacol 1988;37:3987-3993.
    • (1988) Biochem Pharmacol , vol.37 , pp. 3987-3993
    • Dive, C.1    Workman, P.2    Watson, J.V.3
  • 4
    • 0027224112 scopus 로고
    • Can flow cytoenzymology be applied to measure membrane-bound enzyme kinetics? assessment by analysis of gamma-glutamyl transpeptidase activity
    • Dive C, Workman P, Watson JV. Can flow cytoenzymology be applied to measure membrane-bound enzyme kinetics? Assessment by analysis of gamma-glutamyl transpeptidase activity. Biochem Pharmacol 1993;46:643-650.
    • (1993) Biochem Pharmacol , vol.46 , pp. 643-650
    • Dive, C.1    Workman, P.2    Watson, J.V.3
  • 5
    • 0024332109 scopus 로고
    • Inhibition of cellular esterases by the antitumour imidazotetrazines mitozolomide and temozolomide: Demonstration by flow cytometry and conventional spectrofluorimetry
    • Dive C, Workman P, Watson JV. Inhibition of cellular esterases by the antitumour imidazotetrazines mitozolomide and temozolomide: demonstration by flow cytometry and conventional spectrofluorimetry. Cancer Chemother Pharmacol 1989;25(3):149-155.
    • (1989) Cancer Chemother Pharmacol , vol.25 , Issue.3 , pp. 149-155
    • Dive, C.1    Workman, P.2    Watson, J.V.3
  • 6
    • 0029861202 scopus 로고    scopus 로고
    • Coaxial flow mixer for real-time monitoring of cellular responses in flow injection cytometry
    • Blankenstein G, Scampavia LD, Ruzicka J, Christian GD. Coaxial flow mixer for real-time monitoring of cellular responses in flow injection cytometry. Cytometry 1996;25:200-204.
    • (1996) Cytometry , vol.25 , pp. 200-204
    • Blankenstein, G.1    Scampavia, L.D.2    Ruzicka, J.3    Christian, G.D.4
  • 7
    • 0025821141 scopus 로고
    • Time window analysis and sorting
    • Dunne JF. Time window analysis and sorting. Cytometry 1991;12: 597-601.
    • (1991) Cytometry , vol.12 , pp. 597-601
    • Dunne, J.F.1
  • 8
    • 0027934373 scopus 로고
    • Fast kinetic measurements and on line dilution by flow injection
    • Lindberg W, Scampavia LD, Ruzica J, Christian GD. Fast kinetic measurements and on line dilution by flow injection. Cytometry 1994;16:324-330.
    • (1994) Cytometry , vol.16 , pp. 324-330
    • Lindberg, W.1    Scampavia, L.D.2    Ruzica, J.3    Christian, G.D.4
  • 9
    • 0025733149 scopus 로고
    • Flow cytometric monitoring of glutathione content and anthracyclinc retention in tumor cells
    • Nair S, Singh SV, Krishan A. Flow cytometric monitoring of glutathione content and anthracyclinc retention in tumor cells. Cytometry 1991;12(4):336-342.
    • (1991) Cytometry , vol.12 , Issue.4 , pp. 336-342
    • Nair, S.1    Singh, S.V.2    Krishan, A.3
  • 10
    • 0022852609 scopus 로고
    • Quantitative analysis of cellular glutathione by flow cytometry utilizing monochlorobimane: Some applications to radiation and drug resistance in vitro and in vivo
    • Rice GC, Bump EA, Shrieve DC, Lee W, Kovacs M. Quantitative analysis of cellular glutathione by flow cytometry utilizing monochlorobimane: some applications to radiation and drug resistance in vitro and in vivo. Cancer Res 1986;46:6105-6110.
    • (1986) Cancer Res , vol.46 , pp. 6105-6110
    • Rice, G.C.1    Bump, E.A.2    Shrieve, D.C.3    Lee, W.4    Kovacs, M.5
  • 11
    • 0023752554 scopus 로고
    • Heterogeneity of cellular glutathione among cells derived from a murine fibrosarcoma or a human renal cell carcinoma detected by flow cytometric analysis
    • Shrieve DC, Bump EA, Rice GC. Heterogeneity of cellular glutathione among cells derived from a murine fibrosarcoma or a human renal cell carcinoma detected by flow cytometric analysis. J Biol Chem 1988;263(28):14107-14114.
    • (1988) J Biol Chem , vol.263 , Issue.28 , pp. 14107-14114
    • Shrieve, D.C.1    Bump, E.A.2    Rice, G.C.3
  • 12
    • 0028238305 scopus 로고
    • Apparatus of high precision repetitive sequential optical measurement of living cells
    • Deutsch M, Weinreb A. Apparatus of high precision repetitive sequential optical measurement of living cells. Cytometry 1994;16: 214-226.
    • (1994) Cytometry , vol.16 , pp. 214-226
    • Deutsch, M.1    Weinreb, A.2
  • 13
    • 0343531042 scopus 로고    scopus 로고
    • Fluorescence polarization as an early measure of T lymphocyte stimulation
    • Kearse KP, editor. Totowa, NJ: Humana Press; in press
    • Deutsch M, Zurgil N, Kaufman M, Berke G. Fluorescence Polarization as an Early Measure of T Lymphocyte Stimulation. In: Kearse KP, editor. T cell protocols: Development and activation, Totowa, NJ: Humana Press; 1999, in press.
    • (1999) T Cell Protocols: Development and Activation
    • Deutsch, M.1    Zurgil, N.2    Kaufman, M.3    Berke, G.4
  • 14
    • 0029856470 scopus 로고    scopus 로고
    • Decrease of intracellular fluorescein fluorescence polarization in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA) concavaline A (Con A), pokeweed mitogen (PWM) and anti CD3
    • Eisenthal A, Marder O, Dotan D, Baron S, Lifschitz-Mercer B, Chaitchik S, Tirosh R, Weinreb A, Deutsch M. Decrease of intracellular fluorescein fluorescence polarization in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA) concavaline A (Con A), pokeweed mitogen (PWM) and anti CD3. Biol Cell 1996;86:145-150.
    • (1996) Biol Cell , vol.86 , pp. 145-150
    • Eisenthal, A.1    Marder, O.2    Dotan, D.3    Baron, S.4    Lifschitz-Mercer, B.5    Chaitchik, S.6    Tirosh, R.7    Weinreb, A.8    Deutsch, M.9
  • 15
    • 0030927032 scopus 로고    scopus 로고
    • Influenza a virus affects the response of human peripheral blood mononuclear cells to phytohaemagglutinin A by altering the cytoskeleton
    • Eisenthal A, Marder O, Lifschitz-Mercer B, Skornick Y, Fixler D, Avtaiyon R, Tirosh R, Deutsch M. Influenza A virus affects the response of human peripheral blood mononuclear cells to phytohaemagglutinin A by altering the cytoskeleton. Pathobiology 1997;65:69-74.
    • (1997) Pathobiology , vol.65 , pp. 69-74
    • Eisenthal, A.1    Marder, O.2    Lifschitz-Mercer, B.3    Skornick, Y.4    Fixler, D.5    Avtaiyon, R.6    Tirosh, R.7    Deutsch, M.8
  • 16
    • 0031033817 scopus 로고    scopus 로고
    • Fluorescence depolarization as an early measure of lymphocyte stimulation
    • Kaplan MR, Trubniykov E, Berke G. Fluorescence depolarization as an early measure of lymphocyte stimulation. J Immun Meth 1997;201: 15-24.
    • (1997) J Immun Meth , vol.201 , pp. 15-24
    • Kaplan, M.R.1    Trubniykov, E.2    Berke, G.3
  • 17
    • 0017715694 scopus 로고
    • Measurement of cytoplasmic fluorescence depolarization of single cells in a flow system
    • Price GB, McCutcheon MJ, Taylor WB, Miller RG. Measurement of cytoplasmic fluorescence depolarization of single cells in a flow system. J Histochem Cytochem 1977;25:597-600.
    • (1977) J Histochem Cytochem , vol.25 , pp. 597-600
    • Price, G.B.1    McCutcheon, M.J.2    Taylor, W.B.3    Miller, R.G.4
  • 18
    • 0033546730 scopus 로고    scopus 로고
    • The trace and sub-grouping of lymphocyte activation by dynamic fluorescence intensity and polarization measurements
    • Sunray M, Kaufman M, Zurgil N, Deutsch M. The trace and sub-grouping of lymphocyte activation by dynamic fluorescence intensity and polarization measurements. Biochem Biophys Res Comm 1999; 261:712-719.
    • (1999) Biochem Biophys Res Comm , vol.261 , pp. 712-719
    • Sunray, M.1    Kaufman, M.2    Zurgil, N.3    Deutsch, M.4
  • 22
    • 0028307974 scopus 로고
    • Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations
    • Elstein KH, Zucker RM. Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations. Exp Cell Res 1994;211:322-331.
    • (1994) Exp Cell Res , vol.211 , pp. 322-331
    • Elstein, K.H.1    Zucker, R.M.2
  • 24
    • 17544372541 scopus 로고    scopus 로고
    • Anti-metatype antibody stabilization of Fv 4-4-20 variable domain dynamics
    • Carrero J, Mallender WD, Voss EW Jr. Anti-metatype antibody stabilization of Fv 4-4-20 variable domain dynamics. J Biol Chem 1996; 271:11247-11252.
    • (1996) J Biol Chem , vol.271 , pp. 11247-11252
    • Carrero, J.1    Mallender, W.D.2    Voss, E.W.3
  • 25
    • 0028324083 scopus 로고
    • Segmental dynamics of the cytoplasmic domain of erythrocyte hand 3 determined by time-resolved fluorescence anisotropy: Sensitivity to pH and ligand binding
    • Thevenin BJ, Periasamy N, Shohet SB, Verkman AS. Segmental dynamics of the cytoplasmic domain of erythrocyte hand 3 determined by time-resolved fluorescence anisotropy: sensitivity to pH and ligand binding. Proc Natl Acad Sci USA 1994;91:1741-1745.
    • (1994) Proc Natl Acad Sci USA , vol.91 , pp. 1741-1745
    • Thevenin, B.J.1    Periasamy, N.2    Shohet, S.B.3    Verkman, A.S.4
  • 26
    • 1842365540 scopus 로고    scopus 로고
    • Analysis of the intracellular processing of proteins: Application of fluorescence polarization and a novel fluorescent probe
    • Weaver DJ Jr, Durack G, Voss EV Jr. Analysis of the intracellular processing of proteins: Application of fluorescence polarization and a novel fluorescent probe. Cytometry 1997;28:25-35.
    • (1997) Cytometry , vol.28 , pp. 25-35
    • Weaver, D.J.1    Durack, G.2    Voss, E.V.3
  • 27
    • 0007654690 scopus 로고    scopus 로고
    • 2′,7′-Bis-(carboxyethyl)-5-(6′)-carboxyfluorescein (BCECF) as a probe for intracellular fluorescence polarization measurements
    • Gellman Zhornitsky E, Deutsch M, Tirosh R, Yitshak Y, Weinreb A, Shapiro H. 2′,7′-Bis-(carboxyethyl)-5-(6′)-carboxyfluorescein (BCECF) as a probe for intracellular fluorescence polarization measurements. J Biomed Opt 1997;2:186-194.
    • (1997) J Biomed Opt , vol.2 , pp. 186-194
    • Gellman Zhornitsky, E.1    Deutsch, M.2    Tirosh, R.3    Yitshak, Y.4    Weinreb, A.5    Shapiro, H.6
  • 28
    • 0019424339 scopus 로고
    • Intracellular binding of fluorescein in lymphocytes
    • Meisingset KK, Steen HB. Intracellular binding of fluorescein in lymphocytes. Cytometry 1981;14:272-278.
    • (1981) Cytometry , vol.14 , pp. 272-278
    • Meisingset, K.K.1    Steen, H.B.2
  • 29
    • 0018340314 scopus 로고
    • Polarization of fluorescein fluorescence in single cells
    • Udkoff R, Norman A. Polarization of fluorescein fluorescence in single cells. J Histochem Cytochem 1979;27:49-55.
    • (1979) J Histochem Cytochem , vol.27 , pp. 49-55
    • Udkoff, R.1    Norman, A.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.