Decrease of intracellular fluorescein fluorescence polarization (IFFP) in human peripheral blood lymphocytes undergoing stimulation with phytohaemagglutinin (PHA), concanavalin A (ConA), pokeweed mitogen (PWM) and anti-CD3 antibody

Biology of the Cell

Volumn 86, Issue 2-3, 1996, Pages 145-150

  Eisenthal, Avi ^a   Marder, Oleg ^a   Dotan, Dalia ^a   Baron, Shoshana ^a   Lifschitz Mercer, Beatriz ^a   Chaitchik, Samario ^a   Tirosh, Reuven ^b   Weinreb, Arye ^b   Deutsch, Motti ^b  

^a TEL AVIV UNIVERSITY   (Israel)

^b BAR ILAN UNIVERSITY   (Israel)

Author keywords

Activation; Anti CD3; Cytoplasma; Lymphocytes; Mitogen

Indexed keywords

CD3 ANTIGEN; CONCANAVALIN A; FLUORESCEIN; LYMPHOCYTE ANTIBODY; PHYTOHEMAGGLUTININ; POKEWEED MITOGEN; TRITIUM;

ARTICLE; B LYMPHOCYTE; CELL KINETICS; CONTROLLED STUDY; DNA SYNTHESIS; FLUORESCENCE POLARIZATION; HUMAN; HUMAN CELL; LYMPHOCYTE ACTIVATION; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL;

PHYTOLACCACEAE; TRITIUM;

EID: 0029856470     PISSN: 02484900     EISSN: None     Source Type: Journal    
DOI: 10.1016/0248-4900(96)84778-2     Document Type: Article

References (33)

1. 1 Adam LP, Haeberle JR, Hathaway DR (1989) Phosphorylation of caldesmon in arterial smooth muscle. J Biol Chem 264, 7698-7703
2. 2 Ashman RF (1984) Lymphocyte activation. Fundamental Immunology (Paul WE, ed) Raven Press, NY, 267-300
3. 3 Benedict SM, Mills GB, Gelfand EW (1987) IL-2 activates a receptor associated protein kinase. J Immunol 139, 1694-1697
4. 4 Berridge MJ, Irvine RF (1984) Inositol triphosphate, a novel second messenger in cellular signal transduction. Nature (Lond) 312, 315-321
5. 5 Berry N, Nishizuka Y (1990) Protein kinase C and T cell activation. Eur J Biochem 189, 205-214
6. 6 Birx DL, Berger M, Feisher TA (1984) The interference of T cell activation by calcium channel blocking agents. J Immunol 133, 2904-2909
7. 7 Cantrell DA, Smith KA (1984) The interleukin-2 T-cell system: A new cell growth model. Science 224, 1312-1316
8. 2+ in FcR (CD 16)-induced transcription and expression of lymphokine genes. J Exp Med 169, 549-567
9. 9 Cercek L, Cercek B (1977) Application of the phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) in the diagnosis of malignant disorders: A review. Eur J Cancer 13, 903-915
10. 10 Deutsch M, Ron I, Weinreb A, Tirosh R, Chaitchik S (1996) Lymphocyte fluorescence polarization measurements with the Cellscan system: Application to the SCM cancer test. Cytometry 23, 159-165
11. 11 Deutsch M, Weinreb A (1994) Apparatus for high-precision repetitive sequential optical measurement of living cells. Cytometry 16, 214-226
12. 12 Dukovich M, Wano Y, Thuy LB, Katz P, Cullan BR, Kehrl JH, Green WC (1987) A second human interleukin-2 binding protein that may be a component of high-affinity interleukin-2 receptors. Nature 327, 518-522
13. 13 Dupuis G, Martel J, Bastin B, Dion J, Payet MD (1993) Microtubules are not an essential component of phytohemagglutinin-dependent signal transduction in Jurkat T-lymphocytes. Cell Immunol 146, 38-51
14. 14 Eisenthal A, Monselisa T, Singer R, Adler A (1986) Effect of cimetidine on mitogen and MLC responses of PBL from melanoma patients. Cancer Immunol Immunother 21, 141-149
15. 15 Eisental A, Marder O, Lifschitz-Mercer B, Skornick Y, Tirosh R, Weinreb A, Deutsch M, Inhibition of mitogen-induced changes in intracellular fluorescein fluorescence polarization of human peripheral blood lymphocytes by colchicine, vinblastine and cytochalasin B. Cell Struct Funct, in press
16. 16 Gehan EA (1965) Generalized Wilcoxon test for comparing arbitrarily single censored samples. Biometrica 52, 203-208
17. 17 Harnet MM, Klaus GGB (1988) G protein regulation of receptor signalling. Immunol Today 10, 315-320
18. 18 Imboden JB, Shoback DM, Pattison G, Stobo JD (1986) Cholera toxin inhibits the T-cell antigen receptor mediated increases in inositol triphosphate and cytoplasmatic free calcium. Proc Natl Acad Sci USA 83, 5673-5677
19. 19 Kikkawa U, Nishizuka Y (1986) The role of protein kinase C in transmembrane signalling. Annu Rev Cell Biol 2, 149-178
20. 20 Landegren U, Andersson J, Wigzell H (1984) Mechanism of T lymphocyte activation by OKT3 antibodies. A general model for T cell induction. Eur J Immunol 14, 325-331
21. 21 Meuer SC, Hussey RE, Cantrell DA, Hodgdon JC, Schlossman SF, Smith KA, Reinherz EL (1984) Triggering of the T3-T1 antigen-receptor complex results in clonal T-cell proliferation through interleukin 2 dependent autocrine pathway. Proc Natl Acad Sci USA 81, 1509-1513
22. 22 Nishi M, Ishida Y, Honjo T (1988) Expression of functional interleukin-2 receptors in human light chain/TAC transgenic mice. Nature 331, 267-269
23. 23 O'Flynn R, Krensky AM, Beverly PCL, Burakoff SJ, Linen DC (1985) Phytohaemagglutinin activation of T cells through the sheep red blood cell receptor. Nature 313, 318-320
24. 24 Otero A, Bustelo XP, Pichel JG, Freine M, Gomez-Marquez J (1993) Transcript levels of the thymosin beta 4, an actin-sequestering peptide in cell proliferation. Biochim Biophys Acta 1176, 59-63
25. 25 Patya M, Lander HM, Novogrodsky A (1993) Hemin-induced cap formation in lymphocytes: inhibition by protein tyrosine kinase inhibitors. Israel Exp Cell Res 205, 159-164
26. 26 Rabinovitch PS, June CH, Grossmann A, Ledbetter JA (1986) Heterogeneity among T cells in intracellular free calcium responses after mitogen stimulation with PHA or anti-CD3. Simultaneous use of indo-1 and immunofluorescence with flow cytometry. J Immunol 137, 952-961
27. 27 Reed JC, Alpers JC, Nowell PC, Hoover RG (1986) Sequential expression of protooncogenes during lectin-stimulated mitogenesis of normal human lymphocytes. Proc Natl Acad Sci USA 83, 3982-3986
28. 28 Rotman B, Papermaster BW (1966) Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters. Proc Natl Acad Sci USA 55, 134-141
29. 29 Stossel TP (1994) The machinery of cell crawling. When a cell crawls, part of its fluid cytoplasm briefly turns rigid. This transformation depends on the orderly assembly and disassembly of a protein scaffold. Sci Am 260, 40-47
30. 30 Wang HM, Smith KA (1987) The interleukin 2 receptor: Functional consequences of its bimolecular structure. J Exp Med 166, 1055-1069
31. 31 Weiss A, Imboden J, Hardy B, Manger C, Terhost C, Stobo J (1986) The role of the T3/antigen receptor complex in T-cell activation. Annu Rev Immunol 4, 593-619
32. 32 Wright B, Zeidman I, Greig R, Poate G (1985) Inhibition of macrophages by calcium channel blockers and calmodulin antagonists. Cell Immunol 95, 46-53
33. 2+-regulatory protein calmodulin in solution and bound to the membrane skeleton in erythrocyte ghosts: Implications to transmembrane signalling. Biophys J 63, 317-322