TGF-β receptor expression and Smad2 localization are cell density dependent in fibroblasts

Investigative Ophthalmology and Visual Science

Volumn 41, Issue 1, 2000, Pages 89-95

  Petridou, Sevastiani ^a   Maltseva, Olga ^a   Spanakis, Spiro ^a   Masur, Sandra Kazahn ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA ACTIN; ISOPROTEIN; MESSENGER RNA; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA RECEPTOR;

ANIMAL TISSUE; ARTICLE; CELL CONTACT; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CORNEA CELL; FIBROBLAST; MYOFIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RABBIT;

ACTINS; ANIMALS; BLOTTING, NORTHERN; BLOTTING, WESTERN; CELL COUNT; CELL DIFFERENTIATION; CELL NUCLEUS; CELLS, CULTURED; CORNEA; CYTOPLASM; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FIBROBLASTS; RABBITS; RADIOLIGAND ASSAY; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; RNA, MESSENGER; SMAD2 PROTEIN; TRANS-ACTIVATORS; TRANSFORMING GROWTH FACTOR BETA;

EID: 0033986277     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Weimar V. The transformation of corneal stromal cells to fibroblasts in corneal wound healing. Am J Ophthalmol. 1957;44:173-182.
2. Nakayasu K. Stromal changes following removal of epithelium in rat cornea. Jpn J Ophthalmol. 1988;32:113-125.
3. Jester JV, Petroll WM, Barry PA, Cavanagh HD. Expression of α-smooth muscle (α-SM) actin during corneal stromal wound healing. Invest Ophthalmol Vis Sci. 1995;36:809-819.
4. Skalli O, Gabbiani G. The biology of the myofibroblast relationship to wound contraction and fibrocontractive diseases. In: Clark RAF, Henson PM, eds. The Molecular and Cellular Biology of Wound Repair. New York: Plenum; 1988;373-401.
5. Darby I, Skalli O, Gabbiani G. α-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing. Lab Invest. 1990;63:21-29.
6. Sappino AP, Schürch W, Gabbiani G. Biology of disease: differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulations. Lab Invest. 1990; 63:144-161.
7. Ishizaki M, Wakamatsu K. Matsunami T, et al. Dynamics of the expression of cytoskeleton components and adherens molecules by fibroblastic cells in alkali-burned and lacerated corneas. Exp Eye Res. 1994;59:537-549.
8. Grinnell F. Fibroblasts, myofibroblasts, and wound contraction. J Cell Biol. 1994;124:401-404.
9. Petridou S, Masur SK. Immunodetection of connexins and cadherins in corneal fibroblasts and myofibroblasts. Invest Ophthalmol Vis Sci. 1996;37:1740-1748.
10. Masur SK, Connors RL Jr, Cheung JKH, Antohi S. Matrix adhesion characteristics of cultured corneal myofibroblasts. Invest Ophthalmol Vis Sci. 1999;40:904-910.
11. Friedman SL. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. N Engl J Med. 1993;328:1828-1835.
12. Desmoulière A, Geinoz A, Gabbiani F, Gabbiani G. Transforming growth factor-β1 induces α-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol. 1993;122:103-111.
13. Massagué J, Attisano 1, Wrana JL. The TGFβ family and its composite receptors. Trends Cell Biol. 1994;4:172-178.
14. Kretzschmar M, Massagué J. SMADs: mediators and regulators of TGFβ signaling Curr Opin Genet Dev. 1998;8:103-111.
15. Masur SK, Dewal HS, Dinh TT, Erenburg I, Petridou S. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci USA. 1996;93:4219-4223.
16. Rizzino A, Kazakoff P, Ruff E, Kuszynski C, Nebelsick J. Regulatory effects of cell density on the binding of transforming growth factor beta, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor. Cancer Res. 1988;48:4266-4271.
17. Veomett G, Kuszynski C, Kazakoff P, Rizzino A. Cell density regulates the number of cell surface receptors for fibroblast growth factor. Bichem Biophys Res Commun. 1989;159:694-700.
18. Masur SK, Cheung JKH, Antohi S. Identification of integrins in cultured corneal tibroblasts and in isolated keratocytes. Invest Ophthalmol Vis Sci. 1993;34:2690-2698.
19. Kuter I, Johnson-Wint B, Beaupre N, Gross J. Collagenase secretion accompanying changes in cell shape occurs only in the presence of a biologically active cytokine. J Cell Sci. 1989;92:473-485.
20. Freshney RI. Culture of Animal Cells. A Manual of Basic Technique. New York: Alan R. Liss; 1987
21. Kamada S, Kakunaga T. The nucleotide sequence of a human smooth muscle alpha-actin (aortic type) cDNA. Nucleic Acids Res. 1989;17:1767.
22. deLeew W, Slagboom P, Vojg J. Quantitative comparison of mRNA levels in mammalian tissues: 28S ribosomal RNA level as an accurate internal control. Nucleic Acids Res. 1989;17:10137-10138.
23. Wakefield LM. An assay for type-beta transforming growth factor receptor. Methods Enzymol. 1987;146:167-173.
24. Choi ME, Ballermann BJ. Inhibition of capillary morphogenesis and associated apoptosis by dominant negative mutant transforming growth factor-beta receptors. J Biol Chem. 1995;270:21144-21150.
25. Souchelnytskyi S, Tamaki K, Engstrom U, Wernstedt C, ten Dijke P, Heldin CH. Phosphorylation of Ser465 and Ser467 in the C terminus of Smad2 mediates interaction with Smad4 and is required for transforming growth factor-beta signaling. J Biol Chem. 1997;272: 28107-28115.
26. Taubman MB, Smith CW, Izumo S, et al. The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. J Cell Biol. 1989;108: 1799-1806.
27. Maltseva O, Petridou S, Masur SK. Cytokine regulation of corneal fibroblast and myofibroblast phenotypes [ARVO Abstracts]. Invest Ophthalmol Vis Sci. 1998;39(4):S775. Abstract nr 3583.
28. Grainger DJ, Wakefield L, Bethell HW, Farndale RW, Metcalfe JC. Release and activation of platelet latent TGFβ in blood clots during dissolution with plasmin. Nat Med. 1995;1:932-937.
29. Wilson SE, He YG, Weng J, et al. Epithelial injury induces keratocyte apoptosis: hypothesized role for the interleukin-1 system in the modulation of corneal tissue organization and wound healing. Exp Eye Res. 1996;62:325-337.
30. Massagué J. TGfβ signaling: receptors, transducers and Mad proteins. Cell. 1996;85:947-950.