Cloning, expression, and purification of a thermostable nonhomodimeric restriction enzyme, BslI

Journal of Bacteriology

Volumn 182, Issue 4, 2000, Pages 949-955

  Hsieh, Pei Chung ^a   Xiao, Jian Ping ^a   O'Loane, Diana ^a   Xu, Shuang Yong ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STABILITY; ENZYME SUBUNIT; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THERMOSTABILITY;

AMINO ACID SEQUENCE; BACILLUS; CLONING, MOLECULAR; CYTOSINE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; METHYLATION; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; ZINC FINGERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA;

EID: 0033984761     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.4.949-955.2000     Document Type: Article

References (33)

1. Athanasiadis, A., M. Blassi, D. Kotsifaki, P. A. Tucker, K. S. Wilson, and M. Kokkinidis. 1994. Crystal structure of PvuII endonuclease reveals extensive structural homologies to EcoRV. Nat. Struct. Biol. 1:469-475.
2. Bozic, D., S. Grazulis, V. Siksnys, and R. Huber. 1996. Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 Å resolution. J. Mol. Biol. 255:176-186.
3. Cheng, X., K. Balendiran, I. Schildkraut, and J. E. Anderson. 1994. Structure of PuvII endonuclease with cognate DNA. EMBO J. 13:3927-3935.
4. Chong, S., F. B. Mersha, D. G. Comb, M. E. Scott, D. Landry, L. M. Vence, F. B. Perler, J. Benner, R. B. Kucera, and C. A. Hirvonen. 1997. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192:271-281.
5. Dreyer, S. D., L. Zhou, M. A. Machado, W. A. Horton, B. Zabel, A. Winterpacht, and B. Lee. 1998. Cloning, characterization and chromosomal assignment of the human ortholog of murine Zfp-37, a candidate gene for Nager syndrome. Mamm. Genome 9:458-462.
6. Flick, K. E., M. S. Jurica, R. J. Monnat, Jr., and B. L. Stoddard. 1998. DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature 394:96-101.
7. Heitman, J. 1993. On the origins, structures and functions of restriction-modification enzymes. Genet. Eng. (N.Y.) 15:57-108.
8. Holtz, J. K., and M. D. Topal. 1994. Location of putative binding and catalytic sites of NaeI by random mutagenesis. J. Biol. Chem. 269:27286-27290.
9. Janulaitis, A., K. Vaisvila, A. Timinskas, S. Klimasauskas, and V. Butkus. 1992. Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes. Nucleic Acids Res. 20:6051-6056.
10. Joshi, S., and R. Burrows. 1990. ATP synthase complex from bovine heart mitochondria. J. Biol. Chem. 265:14518-14525.
11. Kiss, A., and F. Baldauf. 1983. Molecular cloning and expression in Escherichia coli of two modification methylase genes of Bacillus subtilis. Gene 21:111-119.
12. Kong, H., R. B. Kucera, and W. E. Jack. 1993. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. J. Biol. Chem. 268:1965-1975.
13. Kong, H., and C. L. Smith. 1997. Substrate DNA and cofactor regulate the activities of a multi-functional restriction-modification enzyme, BcgI. Nucleic Acids Res. 25:3687-3692.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
15. Malone, T., R. M. Blumenthal, and X. Cheng. 1995. Structure-guided analysis reveals nine sequence motifs conserved among DNA amino-methyltransferases, and suggests a catalytic mechanism for these enzymes. J. Mol. Biol. 253:618-632.
16. Margolis, P. S., A. Driks, and R. Losick. 1993. Sporulation gene spoIIB from Bacillus subtilis. J. Bacteriol. 175:528-540.
17. Matsudaira, P. 1987. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262: 10035-10038.
18. Morohoshi, F., Y. Ootsuka, K. Arai, H. Ichikawa, S. Mitani, N. Munakata, and M. Ohki. 1998. Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes. Gene 221:191-198.
19. Nelki, D., K. Dudley, P. Cunningham, and M. Akhavan. 1990. Cloning and sequencing of a zinc finger cDNA expressed in mouse testis. Nucleic Acids Res. 18:3655.
20. Newman, M., K. Lunnen, G. Wilson, J. Greci, I. Schildkraut, and S. E. V. Phillips. 1998. Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence. EMBO J. 17:5465-5476.
21. Newman, M., T. Strzelecka, L. F. Dorner, I. Schildkraut, and A. K. Aggarwal. 1995. Structure of BamHI endonuclease bound to DNA: partial folding and unfolding on DNA binding. Science 269:656-663.
22. Newman, M., T. Strzelecka, L. F. Dorner, I. Schildkraut, and A. K. Aggarwal. 1994. Structure of restriction endonuclease BamHI and its relationship to EcoRI. Nature 368:660-664.
23. Pavletich, N. P., and C. O. Pabo. 1991. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 Å. Science 252:809-817.
24. Pountney, D. L., R. P. Tiwari, and J. B. Egan. 1997. Metal and DNA binding properties and mutational analysis of the transcription activating factor of coliphage 186: a prokaryotic C4 zinc-finger protein. Protein Sci. 6:892-902.
25. Roberts, R. J., and D. Macelis. 1998. REBASE-restriction enzymes and methylases. Nucleic Acids Res. 26:338-350.
26. Saleh, M., L. Selleri, P. F. Little, and G. A. Evans. 1992. Isolation and expression of linked zinc finger gene clusters on human chromosome 11q. Genomics 14:970-978.
27. Sethmann, S., P. Ceglowski, J. Willert, R. Iwanicka-Nowicka, T. A. Trautner, and J. Walter. 1999. M.(φ)BssHII, a novel cytosine-C5-DNA-methyltransferase with target-recognizing domains at separated locations of the enzyme. EMBO J. 18:3502-3508.
28. Stankevicius, K., A. Lubjs, A. Timinskas, D. Vailkevicius, and A. Janulaitis. 1998. Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes. Nucleic Acids Res. 26:1084-1091.
29. Szjbalski, W., S. C. Kim, N. Hasan, and A. J. Podhajska. 1991. Class-IIS restriction enzymes - a review. Gene 100:13-26.
30. Wilson, G. 1991. Organization of restriction-modification systems. Nucleic Acids Res. 19:2539-2566.
31. Wilson, G. G., and N. E. Murray. 1991. Restriction and modification systems. Annu. Rev. Genet. 25:585-627.
32. Winkler, F. K., D. W. Banner, D. Tsernoglou, R. S. Brown, S. P. Heathman, R. K. Bryan, P. D. Martin, K. Petratos, and K. S. Wilson. 1993. The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments. EMBO J. 12:1781-1795.
33. Xu, S.-Y., and I. Schildkraut. 1991. Isolation of BamHI variants with reduced cleavage activities. J. Biol. Chem. 206:4425-4429.