An osmotic-remedial, temperature-sensitive mutation in the allosteric activity site of ribonucleotide reductase in Neurospora crassa

Molecular and General Genetics

Volumn 262, Issue 6, 2000, Pages 1022-1035

  Smith, M L ^a   Hubbard, S P ^a   Jacobson, D J ^b   Micali, O C ^a   Glass, N L ^c,d  

^a CARLETON UNIVERSITY   (Canada)

^b STANFORD UNIVERSITY   (United States)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Hydroxyurea; N. crassa; Osmotic remedial temperature sensitive mutation; Repeat induced point mutation (RIP); Ribonucleotide reductase

Indexed keywords

DNA; DNA FRAGMENT; RIBONUCLEOTIDE REDUCTASE;

ALLELE; ALLOSTERISM; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBUNIT; GENE DUPLICATION; GENE LOCUS; GENE MAPPING; GENETIC TRANSFORMATION; HETEROKARYON; NEUROSPORA CRASSA; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OSMOSIS; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN FOLDING; SEQUENCE HOMOLOGY; TEMPERATURE SENSITIVE MUTANT;

NEUROSPORA CRASSA;

EID: 0033980650     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/PL00008645     Document Type: Article

References (39)

1. Akins RA, Lambowitz AM (1985) Genetic methods for cloning Neurospora crassa nuclear genes by complementation of mutants. Mol Cell Biol 5:2272-2278
2. Bairoch A, Apweiler R (1997) The Swiss-Prot protein sequence data bank and its supplement TrEMBL. Nucleic Acids Res 25:31-36
3. Bruchez JJP, Eberle J, Russo VEA (1993) Regulatory sequences in the transcription of Neurospora crassa genes: CAAT box, TATA box, introns, poly(A) tail formation sequences. Fungal Genet Newsl 40:89-96
4. Davis RH, de Serres FJ (1970) Genetic and microbiological techniques of Neurospora crassa. Methods Enzymol 17A:79-143
5. Eckberg M, Sahlin M, Eriksson M, Sjöberg B-M (1996) Two conserved tyrosine residues in protein R1 participate in an intermolecular electron transfer in ribonucleotide reductase. J Biol Chem 271:20655-20659
6. Edelmann SE, Staben C (1994) A statistical analysis of sequence features within genes from Neurospora crassa. Exp Mycol 18:70-81
7. Eklund H, Erikson M, Uhlin U, Nordlund P, Logan D (1997) Ribonucleotide reductase - structural studies of a radical enzyme. J Biol Chem 378:821-825
8. Elledge SJ, Davis RW (1990) Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase. Genes Dev 4:740-751
9. Eriksson M, Uhlin U, Ramaswamy S, Ekberg M, Regnström K, Sjöberg BM, Eklund H (1997) Binding of allosteric effectors to ribonucleotide reductase protein R1: reduction of active-site cysteines promotes substrate binding. Structure 5:1077-1092
10. Fernandez Sarabia M-J, McInerny C, Harris P, Gordon C, Fantes P (1993) The cell cycle genes cdc22 + and suc22 + of the fission yeast Schizosaccharomyces pombe encode the large and small subunits of ribonucleotide reductase. Mol Gen Genet 238:241-251
11. Frohman MA (1990) RACE: Rapid amplification of cDNA ends. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, Toronto, pp 28-38
12. Hawthorne DC, Friis J (1964) Osmotic-remedial mutants. A new classification for nutritional mutants in yeast. Genetics 50:829-839
13. Hershfield MS, Mitchell BS (1995) Immunodeficiency diseases caused by adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency. In: Scriver CR, Beaudet, AL, Sly WS, Valle D (eds) The Metabolic and molecular bases of inherited disease, vol 11. McGraw-Hill, New York, pp 1725-1768
14. Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTAL V: improved software for multiple sequence alignment. CABIOS 8:189-191
15. Jacobson DJ, Ohrnberger J, Atkins RA (1995) The Wilson-Garnjobst heterokaryon incompatibility tester strains of Neurospora crassa contain modifiers which influence growth rate of heterokaryons and distort segregation ratios. Fungal Genet Newsl 42:34-40
16. Jordan A, Reichard P (1998) Ribonucleotide reductases. Annu Rev Biochem 67:71-98
17. Metzenberg RL (1968) Repair of multiple defects of a regulatory mutant of Neurospora by high osmotic pressure and by reversion. Arch Biochem Biophys 125:532-541
18. Mylyk OM (1975) Heterokaryon incompatibility genes in Neurospora crassa detected using duplication-producing chromosome rearrangements. Genetics 80:107-124
19. Nelson MA, et al (1997) Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa. Fungal Genet Biol 21:348-363
20. Nordlund P, Eklund H (1993) Structure and function of the Escherichia coli ribonucleotide reductase protein R2. J Mol Biol 232:123-164
21. Oakley CE, Weil CF, Kretz PL, Oakley BR (1987) Cloning the ribo B locus of Aspergillus nidulans. Gene 53:293-298
22. R marker for fungal library construction and screening. Gene 150:159-162
23. Ormö M, Sjöberg B-M (1996) The Cys292 → Ala substitution in protein R1 of class I ribonucleotide reductase from Escherichia coli has a global effect on nucleotide binding at the specificity-determining allosteric site. Eur J Biochem 241:363-367
24. Perkins DD, Barry EG (1977) The cytogenetics of Neurospora. Adv Genet 19:133-285
25. Perkins DD, Radford A, Newmeyer D, Björkman M (1982) Chromosomal loci of Neurospora crassa. Microbiol Rev 46:426-570
26. Perkins DD, Margolin BS, Selker EU, Haedo SD (1997) Occurrence of repeat-induced point mutation in long segmental duplications of Neurospora. Genetics 147:125-136
27. Reichard P (1993) From RNA to DNA, why so many ribonucleotide reductases? Science 260:1773-1777
28. Royer JC, Yamashiro CT (1992) Generation of transformable spheroplasts from mycelia, macroconidia, microconidia and germinating ascopores of Neurospora crassa. Fungal Genet Newsl 39:76-79
29. Sambrook JE, Fritsch F, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
30. Sanger F, Nicklen S, Coulson B (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
31. + regulatory gene. Proc Natl Acad Sci USA 78:5086-5090
32. Selker EU (1990) Premeiotic instability of repeated sequences in Neurospora crassa. Annu Review Genet 24:579-613
33. Smith ML, Yang CJ, Metzenberg RL, Glass NL (1996) Molecular analysis of escape from het-6 incompatibility in Neurospora crassa partial diploids. Genetics 144:523-531
34. Staben C, Jensen B, Singer M, Pollock J, Schechtman M, Kinsey J, Selker E (1989) Use of a bacterial Hygromycin B resistance gene as a dominant selectable marker in Neurospora crassa transformation. Fungal Genet Newsl 36:79-81
35. Uhlin U, Eklund H (1994) Structure of ribonucleotide reductase protein R1. Nature 370:533-539
36. Vogel HJ (1964) Distribution of lysine pathways among fungi: evolutionary implications. Am Nat 98:435-446
37. Vollmer SJ, Yanofsky C (1986) Efficient cloning of genes of Neurospora crassa. Proc Natl Acad Sci USA 83:4869-4873
38. Westergaard M, Mitchell HK (1947) Neurospora V. A synthetic medium favoring sexual reproduction. Am J Bot 34:573-577
39. Wright JA, Chan AK, Choy BK, Hurta RAR, McClarty GA, Tagger AY (1990) Regulation and drug resistance mechanisms of mammalian ribonucleotide reductase, and the significance to DNA synthesis. Biochem Cell Biol 68:1364-1371