Glutamine synthetase in experimental meningitis: Increased ratio of the subunits 3 and 2 may indicate enhanced activity

Clinica Chimica Acta

Volumn 292, Issue 1-2, 2000, Pages 1-12

  Smirnov, Alexander V ^a   Tumani, Hayrettin ^a   Henne, Sergej ^a   Barchfeld, Sandra ^a   Olgemöller, Ulrike ^a   Wiltfang, Jens ^a   Lange, Peter ^a   Mäder, Michael ^a   Nau, Roland ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Apoptosis, Two dimensional electrophoresis; Glutamine synthetase (EC 6.3.1.2); Meningitis; Streptococcus pneumoniae

Indexed keywords

GLUTAMATE AMMONIA LIGASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BACTERIAL MENINGITIS; BRAIN PROTECTION; CONTROLLED STUDY; DENTATE GYRUS; ENZYME ACTIVITY; ENZYME SUBUNIT; FRONTAL CORTEX; HIPPOCAMPUS; NEOCORTEX; NONHUMAN; PRIORITY JOURNAL; RABBIT; STREPTOCOCCUS PNEUMONIAE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; APOPTOSIS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GLUTAMATE-AMMONIA LIGASE; HIPPOCAMPUS; ISOELECTRIC POINT; MENINGITIS, PNEUMOCOCCAL; MOLECULAR WEIGHT; NEOCORTEX; PROTEIN STRUCTURE, QUATERNARY; RABBITS; TISSUE DISTRIBUTION;

ANIMALIA; BACTERIA (MICROORGANISMS); HIPPOCAMPUS HIPPOCAMPUS; ORYCTOLAGUS CUNICULUS; STREPTOCOCCUS PNEUMONIAE;

EID: 0033974419     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(99)00180-1     Document Type: Article

References (21)

1. Meister A. Glutamine synthetase from mammalian tissues. Method Enzymol. 113:1985;185-199.
2. Norenberg M.D. Distribution of glutamine synthetase in the rat central nervous system. J Histochem Cytochem. 27:1979;756-762.
3. Onley J.M., Ho O.L., Rhee V. Cytotoxic effects of acidic and sulphur-containing amino acids on the infant mouse central nervous system. Exp Brain Res. 14:1971;61-76.
4. Kure S.T., Tominaga T., Yoshimoto K., Tada K., Narisawa K. Glutamate triggers internucleosomal DNA cleavage in neuronal cells. Biochem Biophys Res Comm. 179:1991;39-45.
5. Zysk G., Brück W., Gerber J., Prange H.W., Nau R. Anti-inflammatory treatment influences neuronal apoptotic cell death in the dentate gyrus in experimental pneumococcal meningitis. J Neuropathol Exp Neurol. 55:1996;722-728.
6. Stringaris A.K., Brück W., Tumani H., Schmidt H., Nau R. Increased glutamine synthetase immunoreactivity in experimental pneumococcal meningitis. Acta Neuropathol. 93:1997;215-218.
7. Tumani H., Smirnov A., Barchfeld S., Olgemöller U., Meier K., Lange P., et al. Impaired glutamine synthetase activity increases neuronal apoptosis in dentate gyrus during rabbit meningitis. Poster, 9th European Congress of Clinical Microbiology and Infectious Diseases, Berlin, 21-24.3.1999. Clin Microbiol Infect. 5:(Suppl.3):1999;336.
8. Wiltfang J., Smirnov A., Schnierstein B., Kelemen G., Matties U., Klafki H.W. Improved electrophoretic separation and immunoblotting of beta-amyloid (Aβ) peptides 1-40, 1-42, and 1-43. Electrophoresis. 18:1997;527-532.
9. Wiltfang J., Arold N., Neuhoff V. A new multiphasic buffer system for sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins and peptides with molecular masses 100 000-1000, and their detection with picomolar sensitivity. Electrophoresis. 12:1991;352-366.
10. Heukeshoven J., Dernick R. Improved silver staining procedure for fast staining in PhastSystem Development Unit I. Staining of sodium dodecyl sulfate gels. Electrophoresis. 9:1988;28-32.
11. Neuhoff V., Stamm R., Pardowitz I., Arold N., Ehrhard W., Taube D. Essential problems in quantification of proteins following colloidal staining with coomassie brilliant blue dyes in polyacrylamide gels, and their solution. Electrophoresis. 11:1990;101-117.
12. Tumani H., Qui Shen G., Peter J.B. Purification and immunocharacterisation of human brain glutamine synthetase and its detection in cerebrospinal fluid and serum by a sandwich enzyme immunoassay. J Immunol Method. 188:1995;155-163.
13. Yamamoto H., Konno H., Yamamoto T., Ito K., Mizugaki M., Iwasaki Y. Glutamine synthetase of human brain: purification and characterisation. J Neurochem. 49:1987;603-609.
14. Bokscha I.S., Tereshkina E.B., Burbaeva G.S. Purification and some properties of glutamine synthetase from human brain. Biochemistry (Moscow). 60:1995;1299-1304.
15. Petito C.K., Chung M.C., Verhovsky L.M., Cooper J.L. Brain glutamine synthetase increases following cerebral ischemia in the rat. Brain Res. 569:1992;275-280.
16. Tombaugh G.C., Sapolsky R.M. Hippocampal glutamine synthetase: insensitivity to glucocorticoids and stress. Am J Physiol. 58:1992;E894-E897.
17. Nakamura K., Stadman E.R. Oxidative inactivation of glutamine synthetase subunits. Proc Natl Acad Sci USA. 81:1984;2011-2015.
18. Schor N.F. Inactivation of mammalian brain glutamine synthetase by oxygen radicals. Brain Res. 456:1988;17-21.
19. Butterfield D.A., Hensley K., Cole P., Subramaniam R., Aksenov M., Aksenova M., et al. Oxidatively induced structural alteration of GS assessed by analysis of spin-labeled incorporation kinetics: relevance to Alzheimers disease. J Neurochem. 68:1997;2451-2457.
20. Levine R.L. Oxidative modification of glutamine synthetase. I. Inactivation is due to loss of one histidine residue. J Biol Chem. 258:1983;11823-11827.
21. Folbergrova J., Kiyota Y., Pahlmark K., Memezawa H., Smith M.-L., Siesjo B.K. Does ischemia with reperfusion lead to oxidative damage to proteins in the brain? J Cerebr Blood Flow Metabol. 13:1993;145-152.