One-tube triple staining method for flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation

Cytometry

Volumn 25, Issue 4, 1996, Pages 358-366

  Corver, Willem E ^a   Bonsing, Bert A ^a   Abeln, Edwin C A ^a   Vlak Theil, Petra M ^a   Cornelisse, Cees J ^a   Fleuren, Gert Jan ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Flow cytometry; Keratin; Multiparameter; S phase; Tumor heterogeneity; Tumor marker; Vimentin

Indexed keywords

DNA; KERATIN; TUMOR MARKER; VIMENTIN;

ARTICLE; BREAST CARCINOMA; CANCER CELL; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; OVARY CARCINOMA; PLOIDY; PRIORITY JOURNAL; SOLID TUMOR; TECHNIQUE;

BIOLOGICAL MARKERS; CELL SURVIVAL; COLORING AGENTS; DNA, NEOPLASM; FEMALE; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; GENETIC HETEROGENEITY; HUMANS; INTERMEDIATE FILAMENTS; LASERS; NEOPLASMS; OVARIAN NEOPLASMS; PHENOTYPE; PHYCOERYTHRIN; PLOIDIES; PROLIFERATING CELL NUCLEAR ANTIGEN; PROPIDIUM; STAINING AND LABELING;

EID: 0029905028     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19961201)25:4<358::AID-CYTO7>3.0.CO;2-9     Document Type: Article

References (47)

1. Abeln ECA, Corver WE, Kuipers-Dljkshoorn NJ, Fleuren GJ, Cornelisse CJ Molecular genetic analysis of flow-sorted ovarian tumour cells: Improved detection of loss of heterozygosity. Br J Cancer 70:255-262, 1994.
2. Beerman H, Smit VT, Kluin PM, Bonsing BA, Hermans J, Cornelisse CJ: Flow cytometric analysis of DNA stemline heterogeneity in primary and metastatic breast cancer. Cytometry 12:147-154, 1991.
3. Begg AC, Hofland I: Cell kinetic analysis of mixed populations using three-color fluorescence flow cytometry. Cytometry 12:445-454, 1991
4. Bijman JT, Wagener DJT, Wessels JMC, Van den Broek P, Ramaekers FCS: Cell size, DNA, and cytokeratin analysis of human head and neck tumors by flow cytometry. Cytometry 7:76-81, 1986.
5. Bonsing BA, Beerman H, Kuipers-Dijkshoorn N, Fleuren GJ, Cornelisse CJ: High levels of DNA index heterogeneity in advanced breast carcinomas. Cancer 71:382-391, 1993.
6. Carr NJ, Monihan JM, Nzeako UC, Murakata LA, Sobin LH: Expression of proliferating cell nuclear antigen in hyperplastic polyps, adenomas, and inflammatory cloacogenic polyps of the large intestine. J Clin Pathol 48:46-52, 1995.
7. Coney LR, Tomassetti A, Carayannopoulos L, Frasca V, Kamen BA, Colnaghi MI, Zurawski VR Jr: Cloning of a tumor-associated antigen: MOv18 and MOv19 antibodies recognize a folate-binding protein. Cancer Res 51:6125-6132, 1991.
8. Coney LR, Mezzanzanica D, Sanborn D, Casalini P, Colnaghi MI, Zurawski VR, Jr: Chimeric murine-human antibodies directed against folate-binding receptor are efficient mediators of ovarian carcinoma cell killing. Cancer Res 54:2448-2455, 1994.
9. Corver WE, Cornelisse CJ, Fleuren GJ: Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin, and propidium iodide on a standard FACScan. Cytometry 15:117-128, 1994.
10. Corver WE, Cornelisse CJ, Hermans J, Fleuren GJ: Limited loss of nine tumor-associated surface antigenic determinants after tryptic cell dissociation. Cytometry 19:267-272, 1995.
11. Croonen AM, van der Valk P, Herman CJ, Lindeman J: Cytology, immunopathology, and flow cytometry in the diagnosis of pleural and peritoneal effusions. Lab Invest 58:725-732, 1988.
12. Engelholm SA, Spang Thomsen M, Brunner N, Nohr I, Vindelov LL: Disaggregation of human solid tumours by combined mechanical and enzymatic methods. Br J Cancer 51:93-98, 1985.
13. Ensley JF, Maciorowski Z, Pietraszkiewicz H, Klemic G, KuKuruga M, Sapareto S, Corbett T, Crissman J: Solid tumor preparation for flow cytometry using a standard murine model. Cytometry 8:479-487, 1987.
14. Ensley JF, Maciorowski Z, Pietraszkiewicz H, Hassan M, Kish J, Al Sarraf M, Jacobs J, Weaver A, Atkinson D: Solid tumor preparation for clinical application of flow cytometry. Cytometry 8:488-493, 1987.
15. Ensley JF, Maciorowski Z, Hassan M, Pietraszkiewicz H, Sakr W, Heilbrun LK: Variations in DNA aneuploid cell content during tumor dissociation in human colon and head and neck cancers analyzed by flow cytometry. Cytometry 14:550-558, 1993.
16. Fleuren GJ, Coerkamp EG, Nap M, Van den Broek LJCM, Warnaar SO: Immunohistological characterization of a monoclonal antibody (OV632) against epithelial ovarian carcinomas. Virchows Arch A 410:481-486, 1987.
17. George E, Manivel JC, Dehner LP, Wick MR: Malignant mixed Mullerian tumors: An immunohistochemical study of 47 cases, with histogenetic considerations and clinical correlation. Hum Pathol 22: 215-223, 1991.
18. Giordano M, Danova M, Pellicciari C, Wilson GD, Mazzini G, Conti AM, Franchini G, Riccardi A, Romanini MG: Proliferating cell nuclear antigen (PCNA)/cyclin expression during the cell cycle in normal and leukemic cells. Leuk Res 15:965-974, 1991.
19. Hall PA, Levison DA, Woods AL, Yu CC-W, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R: Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: An index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285-294, 1990.
20. Harrison RF, Reynolds GM, Rowlands DC: Immunohistochemical evidence for the expression of proliferating cell nuclear antigen (PCNA) by nonproliferating hepatocytes adjacent to metastatic tumours and in inflammatory conditions. J Pathol 171:115-122, 1993.
21. Kartner N, Riordan JR, Ling V: Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines. Science 221: 1285-1288, 1983.
22. Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S phase probes in flow cytometric cell cycle analysis. Cancer Res 51:4570-4574, 1991.
23. Landberg G, Roos G: Flow cytometric analysis of proliferation associated nuclear antigens using washless staining of unfixed cells. Cytometry 13:230-240, 1992.
24. Li BDL, Harlow SP, Budnick RM, Sheedy DL, Stewart CC: Detection of HER-2/neu oncogene amplification in flow cytometry-sorted breast ductal cells by competitive polymerase chain reaction. Cancer 73:2771-2778, 1994.
25. Litvinov SV, Velders MP, Bakker HAM, Fleuren GJ, Warnaar SO: Ep-CAM: A human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol 125:437-446, 1994.
26. Looijenga LHJ, Oosterhuis JW, Ramaekers FCS, De Jong B, Dam A, Beck JLM, Sleijfer DT, Schraffordt Koops H: Dual parameter now cytometry for deoxyribonucleic acid and intermediate filament proteins of residual mature teratoma. Lab Invest 64:113-117, 1991.
27. Mantovani LT, Miotti S, Ménard S, Canevari S, Raspagliesi F, Bottini C, Bottero F, Colnaghi MI: Folate-binding protein distribution in normal tissues and biological fluids from ovarian carcinoma patients as detected by the monoclonal antibodies MOv18 and MOv19. Eur J Cancer 30A:363-369, 1994.
28. Oud PS, Henderik JBJ, Beck HLM, Veldhuizen JAM, Vooijs GP, Herman CJ, Ramaekers FCS: Flow cytometric analysis and sorting of human endometrial cells after immunocytochemical labeling for cytokeratin using a monoclonal antibody. Cytometry 6:159-164, 1985.
29. Pak KY, Nedelman MA, Fogler WE, Tam SH, Wilson E, Van Haarlem LJM, Colognola R, Warnaar SO, Daddona PE: Evaluation of the 323/A3 monoclonal antibody and the use of technetium-99m-labeled 323/A3 Fab' for the detection of pan adenocarcinoma. Nucl Med Biol 18:483-497, 1991.
30. Park CH, Kimler BF: Tumor cell-selective flow cytometric analysis for DNA content and cytokeratin expression of clinical tumor specimens by "cross-gating." Anticancer Res 14:29-36, 1994.
31. Poels LG, Peters D, Van Megen Y, Vooijs GP, Verheyen RNM, Willemen A, Van Niekerk CC, Jap PHK, Mungyer G: Monoclonal antibody against human ovarian tumor-associated antigens. J Natl Cancer Inst 76:781-791, 1986.
32. Ramaekers FCS, Beck HL, Feitz WF, Oud PS, Debruyne FM, Vooijs GP, Herman CJ: Application of antibodies to intermediate filament proteins as tissue-specific probes in the flow cytometric analysis of complex tumors. Anal Quant Cytol Histol 8:271-280, 1986.
33. Sasaki DT, Dumas SE, Engleman EG: Discrimination of viable and nonviable cells using propidium iodide in two color immunofluorescence. Cytometry 8:413-420, 1987.
34. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgl JV: Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13:204-208, 1992.
35. Schutte B, Tinnemans MMFJ, Pijpers GFP, Lenders MHJH, Ramaekers FCS: Three parameter flow cytometric analysis for simultaneous detection of cytokeratin, proliferation associated antigens, and DNA content. Cytometry 21:177-186, 1995.
36. Slocum HK, Pavelic ZP, Rustum YM, Creaven PJ, Karakousis C, Takita H, Greco WR: Characterization of cells obtained by mechanical and enzymatic means from human melanoma, sarcoma, and lung tumors. Cancer Res 41:1428-1434, 1981.
37. Stonesifer KJ, Xiang J, Wilkinson EJ, Benson NA, Braylan RC: Flow cytometric analysis and cytopathology of body cavity fluids. Acta Cytol 31:125-130, 1987.
38. Van Dam PA, Watson JV, Lowe DG, Chard T, Shepherd JH: Comparative evaluation of fresh, fixed, and cryopreserved solid tumor cells for reliable flow cytometry of DNA and tumor associated antigen. Cytometry 13:722-729, 1992.
39. Van der Linden JC, Herman CJ, Boenders JGC, Van de Sandt MM, Lindeman J: Flow cytometric DNA content of fresh tumor specimens using keratin-antibody as second stain for two-parameter analysis. Cytometry 13:163-168, 1992.
40. Van Niekerk CC, Boerman OC, Ramaekers FCS, Poels LG: Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas. Am J Pathol 138:455-463, 1991.
41. Vindelov LL, Christensen IJ, Nissen NI: A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 3:323-327, 1983.
42. Visscher DW, Sochacki P, Ottosen S, Wykes S, Crissman JD: Assessment and significance of diploid-range epithelial populations in DNA aneuploid breast carcinomas using multi-parameter flow cytometry. Anal Cell Pathol 8:267-277, 1995.
43. Visscher DW, Zarbo RJ, Jacobsen G, Kambouris A, Talpos G, Sakr W, Crissman JD: Multiparameter deoxyribonucleic acid and cell cycle analysis of breast carcinomas by flow cytometry. Lab Invest 62:370-378, 1990.
44. Wingren S, Stål O, Carstensen J, Sun X-F, Nordenskjöld B: S-phase determination of immunoselected cytokeratin-containing breast cancer cells improves the prediction of recurrence. Breast Cancer Res Treat 29:179-187, 1994.
45. Wingren S, Stål O, Nordenskjöld B: Flow cytometric analysis of S-phase fraction in breast carcinomas using gating on cells containing cytokeratin. Br J Cancer 69:546-549, 1994.
46. Zarbo RJ, Visscher D, Crissman JD: Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen. Anal Quant Cytol Histol 11:391-402, 1989.
47. Zarbo RJ, Brown RD, Linden MD, Torres FX, Nakhleh RE, Schultz DS: Rapid (one-shot) staining method for two-color multiparametric DNA flow cytometric analysis of carcinomas using staining for cytokeratin and leukocyte common antigen. Am J Clin Pathol 101: 638-642, 1994.