Structure-activity relationship of truncated and substituted analogues of the intracellular delivery vector Penetratin

Journal of Peptide Research

Volumn 55, Issue 2, 2000, Pages 163-172

  Fischer, Peter M ^a   Zhelev, N Z ^a   Wang, S ^a   Melville, J E ^a   Fahraeus R ^a   Lane, D P ^a  

^a CYCLACEL LTD   (United Kingdom)

Author keywords

Antennapedia homeoprotein; Cell internalization; Delivery vector; Membrane translocation; Penetratin

Indexed keywords

ALANINE; HOMEODOMAIN PROTEIN; PENETRATIN; PENETRATIN DERIVATIVE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DROSOPHILA; HUMAN; HUMAN CELL; INTERNALIZATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; STRUCTURE ACTIVITY RELATION;

ANTENNAPEDIA HOMEODOMAIN PROTEIN; BIOLOGICAL TRANSPORT; CELL COMPARTMENTATION; CELL NUCLEUS; CELLS, CULTURED; CYTOSOL; DRUG CARRIERS; HOMEODOMAIN PROTEINS; HUMANS; MOLECULAR CONFORMATION; NUCLEAR PROTEINS; OLIGOPEPTIDES; PEPTIDE FRAGMENTS; PEPTIDES, CYCLIC; STRUCTURE-ACTIVITY RELATIONSHIP; TRANSCRIPTION FACTORS;

EID: 0033973662     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3011.2000.00163.x     Document Type: Article

References (15)

1. 1. Derossi, D., Joliot, A.H., Chassaing, G. & Prochiantz, A. (1994) The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Biol. Chem. 269, 10444-10450.
2. 2. Vivès, E., Brodin, P. & Lebleu, B. (1997) A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J. Biol. Chem. 272, 16010-16017.
3. 3. Pooga, M., Hällbrink, M., Zorko, M. & Langel, Ü. (1998) Cell penetration by transportan. FASEB J. 12, 67-77.
4. 4. Gehring, W.J., Müller, M., Affolter, M., Percival-Smith, A., Billeter, M., Qian, Y.Q., Otting, G. & Wüthrich, K. (1990) The structure of the homeodomain and its functional implications. Trends Genet. 6, 323-329.
5. 5. Le Roux, I., Joliot, A.H., Bloch-Gallego, E., Prochiantz, A. & Volovitch, M. (1993) Neurotrophic activity of the Antennapedia homeodomain depends on its specific DNA-binding properties. Proc. Natl Acad. Sci. USA 90, 9120-9124.
6. 6. Kato, D., Miyazawa, K., Ruas, M., Starborg, M., Wada, I., Oka, T., Sakai, T., Peters, G. & Hara, E. (1998) Features of replicative senescence induced by direct addition of Antennapedia-p16INK4A fusion protein human diploid fibroblasts. FEBS Lett. 427, 203-208.
7. 7. Pooga, M., Soomets, U., Hällbrink, M., Valkna, A., Saar, K., Rezaei, K., Kahl, U., Hao, J.-X., Xu, X.-J., Wiesenfeld-Hallin, Z., Hokfelt, T., Bartfai, T. & Langel Ü. (1998) Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo. Nature Biotechnol. 16, 857-861.
8. 8. Derossi, D., Chassaing, G. & Prochiantz, A. (1998) Trojan peptides: the Penetratin system for intracellular delivery. Trends Cell Biol. 8, 84-87.
9. 9. Derossi, D., Calvet, S., Trembleau, A., Brunissen, A., Chassaing, G. & Prochiantz, A. (1996) Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent. J. Biol. Chem. 271, 18188-18193.
10. 10. King, D.S., Fields, C.G. & Fields, G.B. (1990) A cleavage method which minimizes side reactions following Fmoc solid phase peptide synthesis. Int. J. Peptide Protein Res. 36, 255-266.
11. 11. Beavis, R.C., Chaudhary, T. & Chait, B.T. (1992) α-Cyano-4-hydroxycinnamic acid as a matrix for matrix-assisted laser desorption mass spectrometry. Org. Mass Spectrometry 27, 156-158.
12. 12. Valerio, R.M., Bray, A.M., Campbell, R.A., Dipasquale, A., Margellis, C., Rodda, S.J., Geysen, H.M. & Maeji, N.J. (1993) Multipin peptide synthesis at the micromole scale using 2-hydroxyethyl methacrylate grafted polyethylene supports. Int. J. Peptide Protein Res. 42, 1-9.
13. 4I for the reduction of methionine sulfoxide in peptides containing cysteine and cystine. Tetrahedron 51, 5701-5710.
14. 14. Brugidou, I., Legrand, C., Mery, J. & Rabie, A. (1995) The retro-inverso form of a homeobox-derived short peptide is rapidly internalised by cultured neurones: a new basis for an efficient intracellular delivery system. Biochem. Biophys. Res. Commun. 214, 685-693.
15. 15. Berlose, J.P., Convert, O., Derossi, D., Brunissen, A. & Chassaing, G. (1996) Conformational and associative behaviours of the third helix of Antennapedia homeodomain in membrane-mimetic environments. Eur. J. Biochem. 242, 372-386.